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Clostridium difficile in Adult Patients with Nosocomial Diarrhea in a Costa Rican Hospital

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  • 1 Laboratorio de Investigación en Bacteriología Anaerobia, Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, San José, Costa Rica
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Stool samples from 104 adult patients with nosocomial antibiotic–associated diarrhea were analyzed for Clostridium difficile by cultivation, toxin A inmunoenzymatic detection, and toxin B cytotoxic detection. The isolates were additionally screened for the toxin genes by polymerase chain reaction. C. difficile was isolated from 26 samples, and the toxins were directly detected in another 5 samples. Toxin A and B genes were detected in all toxigenic bacterial isolates. The detection rate of 30% indicates that C. difficile is a major etiologic agent of nosocomial diarrhea in Costa Rica.

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