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-
1
Russell PK, Nisalak A, Sukhavachana P, Vivona S, 1967. A plaque reduction test for dengue virus neutralization antibodies. J Immunol 99 :285–290.
-
2
Halstead SB, Rojanasuphot S, Sangkawibha N, 1983. Original antigenic sin in dengue. Am J Trop Med Hyg 32 :154–156.
-
3
Endy TP, Nisalak A, Chunsuttitwat S, Vaughn DW, Green S, Ennis FA, Rothman AL, Libraty DH, 2004. Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort study of DV infection in Thailand. J Infect Dis 189 :990–1000.
-
4
Vorndam V, Beltran M, 2002. Enzyme-linked immunosorbent assay-format microneutralization test for dengue viruses. Am J Trop Med Hyg 66 :208–212.
-
5
Martin NC, Pardo J, Simmons M, Tjaden JA, Widjaja S, Marovich MA, Sun W, Porter KR, Burgess TH, 2006. An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies. J Virol Methods 134 :74–85.
-
6
Tassaneetrithep B, Burgess TH, Granelli-Piperno A, Trumpfheller C, Finke J, Sun W, Eller MA, Pattanapanyasat K, Sarasombath S, Birx DL, Steinman RM, Schlesinger S, Maravich MA, 2003. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med 197 :823–829.
-
7
Sun W, Edelman R, Kanesathasan N, Eckels KH, Putnak JR, King AD, Houng HS, Tang D, Scherer JM, Hoke CH Jr, Innis BL, 2003. Vaccination of human volunteers with monovalent and tetravalent live-attenuated dengue vaccine candidates. Am J Trop Med Hyg 69 :24–31.
-
8
Halstead SB, Marchette NJ, 2003. Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii. Am J Trop Med Hyg 69 :5–11.
-
9
Kalayanarooj S, Vaughn DW, Nimmannitya S, Green S, Suntayakorn S, Kunentrasai N, Viramitrachai W, Ratanachu-eke S, Kiatpolpoj S, Innis BL, Rothman AL, Nisalak A, Ennis FA, 1997. Early clinical and laboratory indicators of acute dengue illness. J Infect Dis 176 :313–321.
-
10
Raengsakulrach B, Nisalak A, Maneekarn N, Yenchitsomanus PT, Limsomwong C, Jairungsri A, Thirawuth V, Green S, Kalayanarooj S, Suntayakorn S, Sittisombut N, Malasit P, Vaughn D, 2002. Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens. J Virol Methods 105 :219–232.
-
11
Singh KR, Paul SD, 1969. Isolation of dengue viruses in Aedes albopictus cell cultures. Bull World Health Organ 40 :982–983.
-
12
Clarke DH, Casals J, 1958. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7 :561–573.
-
13
Kuno G, Gomez I, Gubler D, 1991. An ELISA procedure for the diagnosis of dengue infections. J Virol Methods 33 :101–113.
-
14
Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, Puttisri P, Hoke CH, 1989. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg 40 :418–427.
-
15
Perez JL, de Ona M, Niubo J, Villar H, Melon S, Garcia A, Martin RJ, 1995. Comparison of several fixation methods for cytomegalovirus antigenemia assay. Clin Microbiol 33 :1646–1649.
-
16
Ferguson M, Yoksan S, 2002. Preparation of Anti-Dengue Reference Human Serum. Report of the Steering Committee on Dengue and Other Flavivirus Vaccines. Geneva: World Health Organization.
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Comparative Evaluation of Three Assays for Measurement of Dengue Virus Neutralizing Antibodies
J. Robert Putnak
J. Robert Putnak
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Rafael de la Barrera
Rafael de la Barrera
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Timothy Burgess
Timothy Burgess
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Jorge Pardo
Jorge Pardo
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Francis Dessy
Francis Dessy
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Dirk Gheysen
Dirk Gheysen
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Yves Lobet
Yves Lobet
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Sharone Green
Sharone Green
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Timothy P. Endy
Timothy P. Endy
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Stephen J. Thomas
Stephen J. Thomas
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Kenneth H. Eckels
Kenneth H. Eckels
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Bruce L. Innis
Bruce L. Innis
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Wellington Sun
Wellington Sun
Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland; Naval Medical Research Center, Silver Spring, Maryland; Global Vaccine Development, GlaxoSmithKline Biologicals, Rixensart, Belgium; Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worchester, Massachusetts; Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
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Plaque reduction neutralization tests (PRNTs) are commonly used for measuring levels of dengue virus (DENV) neutralizing antibodies. However, these assays lack a standardized format, generally have a low sample throughput, and are labor-intensive. The objective of the present study was to evaluate two alternative DENV neutralizing antibody assays: an enzyme-linked immunosorbent assay–based microneutralization (MN) assay, and a fluorescent antibody cell sorter–based, DC-SIGN expresser dendritic cell (DC) assay. False-positive rates, serotype specificity, reproducibility, sensitivity, and agreement among the assay methods were assessed using well-characterized but limited numbers of coded test sera. Results showed that all three assays had false-positive rates of less than 10% with titers near the cut-off and generally below the estimated limits of detection. All three methods demonstrated a high degree of specificity and good agreement when used to assay sera and serum mixtures from monovalent vaccinees and sera from patients after primary natural infection, with the only notable exception being moderate-to-high neutralizing antibody titers against DENV 2 measured by PRNT in a mixture containing only DENV 3 and DENV 4 sera. The MN and DC assays demonstrated good reproducibility. All three assays were comparable in their sensitivity, except that the PRNT was less sensitive for measuring DENV 4 antibody, and the MN and DC assays were less sensitive for measuring DENV 2 antibody. However, when used to test sera from persons after tetravalent DENV vaccination or secondary DENV infection, there was poor specificity and poor agreement among the different assays.
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-
1
Russell PK, Nisalak A, Sukhavachana P, Vivona S, 1967. A plaque reduction test for dengue virus neutralization antibodies. J Immunol 99 :285–290.
-
2
Halstead SB, Rojanasuphot S, Sangkawibha N, 1983. Original antigenic sin in dengue. Am J Trop Med Hyg 32 :154–156.
-
3
Endy TP, Nisalak A, Chunsuttitwat S, Vaughn DW, Green S, Ennis FA, Rothman AL, Libraty DH, 2004. Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort study of DV infection in Thailand. J Infect Dis 189 :990–1000.
-
4
Vorndam V, Beltran M, 2002. Enzyme-linked immunosorbent assay-format microneutralization test for dengue viruses. Am J Trop Med Hyg 66 :208–212.
-
5
Martin NC, Pardo J, Simmons M, Tjaden JA, Widjaja S, Marovich MA, Sun W, Porter KR, Burgess TH, 2006. An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies. J Virol Methods 134 :74–85.
-
6
Tassaneetrithep B, Burgess TH, Granelli-Piperno A, Trumpfheller C, Finke J, Sun W, Eller MA, Pattanapanyasat K, Sarasombath S, Birx DL, Steinman RM, Schlesinger S, Maravich MA, 2003. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med 197 :823–829.
-
7
Sun W, Edelman R, Kanesathasan N, Eckels KH, Putnak JR, King AD, Houng HS, Tang D, Scherer JM, Hoke CH Jr, Innis BL, 2003. Vaccination of human volunteers with monovalent and tetravalent live-attenuated dengue vaccine candidates. Am J Trop Med Hyg 69 :24–31.
-
8
Halstead SB, Marchette NJ, 2003. Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii. Am J Trop Med Hyg 69 :5–11.
-
9
Kalayanarooj S, Vaughn DW, Nimmannitya S, Green S, Suntayakorn S, Kunentrasai N, Viramitrachai W, Ratanachu-eke S, Kiatpolpoj S, Innis BL, Rothman AL, Nisalak A, Ennis FA, 1997. Early clinical and laboratory indicators of acute dengue illness. J Infect Dis 176 :313–321.
-
10
Raengsakulrach B, Nisalak A, Maneekarn N, Yenchitsomanus PT, Limsomwong C, Jairungsri A, Thirawuth V, Green S, Kalayanarooj S, Suntayakorn S, Sittisombut N, Malasit P, Vaughn D, 2002. Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens. J Virol Methods 105 :219–232.
-
11
Singh KR, Paul SD, 1969. Isolation of dengue viruses in Aedes albopictus cell cultures. Bull World Health Organ 40 :982–983.
-
12
Clarke DH, Casals J, 1958. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7 :561–573.
-
13
Kuno G, Gomez I, Gubler D, 1991. An ELISA procedure for the diagnosis of dengue infections. J Virol Methods 33 :101–113.
-
14
Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, Puttisri P, Hoke CH, 1989. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg 40 :418–427.
-
15
Perez JL, de Ona M, Niubo J, Villar H, Melon S, Garcia A, Martin RJ, 1995. Comparison of several fixation methods for cytomegalovirus antigenemia assay. Clin Microbiol 33 :1646–1649.
-
16
Ferguson M, Yoksan S, 2002. Preparation of Anti-Dengue Reference Human Serum. Report of the Steering Committee on Dengue and Other Flavivirus Vaccines. Geneva: World Health Organization.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 505 | 467 | 196 |
| Full Text Views | 309 | 7 | 1 |
| PDF Downloads | 132 | 6 | 0 |