First Isolation of West Nile Virus in the Caribbean

Roberto Barrera Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Elizabeth Hunsperger Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Jorge L. Muñoz-Jordán Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Manuel Amador Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Annette Diaz Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Joshua Smith Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Kovi Bessoff Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Manuela Beltran Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Edgardo Vergne Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Mark Verduin Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Amy Lambert Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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Wellington Sun Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920

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A sentinel chicken program for West Nile virus (WNV) surveillance was initiated in July 2006 in eastern Puerto Rico, yielding the first seroconversions on June 4, 2007. WNV was isolated from sentinel chicken serum and mosquito pools (Culex nigripalpus, Culex bahamensis) for the first time in Tropical America. Preliminary sequence analysis of the prM and E genes revealed a 1-amino acid difference (V159A) between the Puerto Rican 2007 and the NY99. This mutation has been observed in the current dominant clade circulating in the United States. Sentinel chicken surveillance was a useful tool for the detection of West Nile virus in the tropics.

Author Notes

Reprint requests: Roberto Barrera, Dengue Branch, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico 00920, Telephone: +1 787-706-2399, Fax: +1 787-706-2496, E-mail: rbarrera@cdc.gov.
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