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-
1
Esquivel ML, Segura EL, 1994. Calculating the number of patients with Chagas disease in Argentina. Medicina (B Aires) 54 :91–92.
-
2
Gurtler RE, Segura EL, Cohen JE, 2003. Congenital transmission of Trypanosoma cruzi infection in Argentina. Emerg Infect Dis 9 :29–32.
-
3
Blanco SB, Segura EL, Gurtler RE, 1999. Control of congenital transmission of Trypanosoma cruzi in Argentina. Medicina (B Aires) 59 (Suppl 2):138–142.
-
4
Freilij HL, Altcheh JM, Corral RS, 1990. Respuesta al tratamiento en niños con infección chagásica congénita en zona no endémica. Medicina (B Aires) 50 :389.
-
5
Winker P, Telleria J, Bosseno MF, Cardoso MA, Marques P, Yaksic N, Aznar C, Liegeard P, Hotebeyrie M, Noireau F, Morel CM, Breniere SF, 1997. PCR-based diagnosis for Cha-gas disease in Bolivian children living in an active transmission area: comparison with conventional serological and parasitological diagnosis. Parasitology 114 :367–373.
-
6
Diez C, Manattini S, Imaz MS, Zanuttini JC, Marcipar A, 1998. PCR (polymerase chain reaction) in neonatal Chagas disease. An alternative for its early diagnosis? Medicina (B Aires) 58 :436–437.
-
7
Russomando G, Tomassone MM, de Guillen I, Acosta N, Vera N, Almiron M, Candia N, Calcena MF, Figueredo A, 1998. Treatment of congenital Chagas’ disease diagnosed and followed up by the polymerase chain reaction. Am J Trop Med Hyg 59 :487–491.
-
8
Mora MC, Sanchez Negrette O, Marco D, Barrio A, Ciaccio M, Segura MA, Basombrio MA, 2005. Early diagnosis of congenital Trypanosoma cruzi infection using PCR, hemoculture, and capillary concentration, as compared with delayed serology. J Parasitol 91 :1468–1473.
-
9
Conclusions of Round Tables and Synopsis of an International Colloquium, 2003. Congenital infection with Trypanosoma cruzi: from mechanisms of transmission to strategies for diagnosis and control. Rev Soc Bras Med Trop 36 :767–771.
-
10
Freilij H, Muller L, González Cappa SM, 1983. Direct micromethod for diagnosis of acute and congenital Chagas‘ disease. J Clin Microbiol 18 :327–330.
-
11
Britto C, Cardoso MA, Wincker P, Morel CM, 1993. A simple protocol for the physical cleavage of Trypansoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas’ disease. Mem Inst Oswaldo Cruz 88 :171–172.
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12
Wincker P, Brito C, Borges Pereira J, Cardoso MA, Oelemann W, Morel CM, 1994. Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51 :133–139.
-
13
Virreira M, Torrico F, Truyens C, Alonso-Vega C, Solano M, Carlier Y, Svoboda M, 2003. Comparison of polymerase chain reaction methods for reliable and easy detection of congenital Trypanosoma cruzi infection. Am J Trop Med Hyg 68 :574–582.
-
14
Hermann E, Truyens C, Alonso-Vega J, Rodriguez P, Berthe A, Gonzalez-Merino E, Torrico F, Carlier Y, 2002. Human fetuses are able to mount an adultlike CD8 T-cell response. Blood 100 :2153–2158.
-
15
Streiger M, Fabbro D, del Barco M, Beltramino R, Bovero N, 1995. Congenital Chagas disease in the city of Santa Fe. Diagnosis and treatment. Medicina (B Aires) 55 :125–132.
-
16
Schijman AG, Altcheh J, Burgos JM, Biancardi M, Bisio M, Levin MJ, Freilij HJ, 2003. Aetiological treatment of congenital Chagas’ disease diagnosed and monitored by the polymerase chain reaction. J Antimicrob Chemother 52 :441–449.
-
17
Sánchez Negrette O, Mora MC, Basombrío MA, 2005. High prevalence of congenital Trypanosoma cruzi infection and family clustering in Salta, Argentina. Pediatrics 115 :668–672.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 272 | 219 | 15 |
| Full Text Views | 270 | 12 | 0 |
| PDF Downloads | 50 | 9 | 0 |
The Value of Molecular Studies for the Diagnosis of Congenital Chagas Disease in Northeastern Argentina
Cristina N. Diez
Cristina N. Diez
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Hospital Central Reconquista, Santa Fe, Argentina; Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina
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Silvia Manattini
Silvia Manattini
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Hospital Central Reconquista, Santa Fe, Argentina; Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina
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Juan Carlos Zanuttini
Juan Carlos Zanuttini
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Hospital Central Reconquista, Santa Fe, Argentina; Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina
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Oscar Bottasso
Oscar Bottasso
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Hospital Central Reconquista, Santa Fe, Argentina; Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina
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Iván Marcipar
Iván Marcipar
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Hospital Central Reconquista, Santa Fe, Argentina; Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina
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Polymerase chain reaction (PCR) as a diagnostic tool for congenital Chagas disease is scarcely used. To assess the usefulness of PCR, a study in neonates was carried out to compare PCR with standard methods; that is bloodstream parasites by microhematocrit, and/or seroconversion by two conventional procedures. Seventeen neonates, born to mothers with positive Trypanosoma cruzi serology were analyzed by serologic, parasitological and molecular procedures, in blood samples taken at birth, and when aged 2–4 and 9 months. For a better comparison of sensitivity between both parasitological methods (microhematocrit and PCR), the number of samples of neonates studied at birth extended to 121. In children followed-up until 9 months of age, a 100% correlation between PCR and routine diagnosis was found, with PCR showing its highest sensitivity from Month 2 onwards. Comparative analysis between both parasitological methods, on samples taken at birth, showed a higher sensitivity of PCR respect the microhematocrit (P < 0.0008).
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-
1
Esquivel ML, Segura EL, 1994. Calculating the number of patients with Chagas disease in Argentina. Medicina (B Aires) 54 :91–92.
-
2
Gurtler RE, Segura EL, Cohen JE, 2003. Congenital transmission of Trypanosoma cruzi infection in Argentina. Emerg Infect Dis 9 :29–32.
-
3
Blanco SB, Segura EL, Gurtler RE, 1999. Control of congenital transmission of Trypanosoma cruzi in Argentina. Medicina (B Aires) 59 (Suppl 2):138–142.
-
4
Freilij HL, Altcheh JM, Corral RS, 1990. Respuesta al tratamiento en niños con infección chagásica congénita en zona no endémica. Medicina (B Aires) 50 :389.
-
5
Winker P, Telleria J, Bosseno MF, Cardoso MA, Marques P, Yaksic N, Aznar C, Liegeard P, Hotebeyrie M, Noireau F, Morel CM, Breniere SF, 1997. PCR-based diagnosis for Cha-gas disease in Bolivian children living in an active transmission area: comparison with conventional serological and parasitological diagnosis. Parasitology 114 :367–373.
-
6
Diez C, Manattini S, Imaz MS, Zanuttini JC, Marcipar A, 1998. PCR (polymerase chain reaction) in neonatal Chagas disease. An alternative for its early diagnosis? Medicina (B Aires) 58 :436–437.
-
7
Russomando G, Tomassone MM, de Guillen I, Acosta N, Vera N, Almiron M, Candia N, Calcena MF, Figueredo A, 1998. Treatment of congenital Chagas’ disease diagnosed and followed up by the polymerase chain reaction. Am J Trop Med Hyg 59 :487–491.
-
8
Mora MC, Sanchez Negrette O, Marco D, Barrio A, Ciaccio M, Segura MA, Basombrio MA, 2005. Early diagnosis of congenital Trypanosoma cruzi infection using PCR, hemoculture, and capillary concentration, as compared with delayed serology. J Parasitol 91 :1468–1473.
-
9
Conclusions of Round Tables and Synopsis of an International Colloquium, 2003. Congenital infection with Trypanosoma cruzi: from mechanisms of transmission to strategies for diagnosis and control. Rev Soc Bras Med Trop 36 :767–771.
-
10
Freilij H, Muller L, González Cappa SM, 1983. Direct micromethod for diagnosis of acute and congenital Chagas‘ disease. J Clin Microbiol 18 :327–330.
-
11
Britto C, Cardoso MA, Wincker P, Morel CM, 1993. A simple protocol for the physical cleavage of Trypansoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas’ disease. Mem Inst Oswaldo Cruz 88 :171–172.
-
12
Wincker P, Brito C, Borges Pereira J, Cardoso MA, Oelemann W, Morel CM, 1994. Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51 :133–139.
-
13
Virreira M, Torrico F, Truyens C, Alonso-Vega C, Solano M, Carlier Y, Svoboda M, 2003. Comparison of polymerase chain reaction methods for reliable and easy detection of congenital Trypanosoma cruzi infection. Am J Trop Med Hyg 68 :574–582.
-
14
Hermann E, Truyens C, Alonso-Vega J, Rodriguez P, Berthe A, Gonzalez-Merino E, Torrico F, Carlier Y, 2002. Human fetuses are able to mount an adultlike CD8 T-cell response. Blood 100 :2153–2158.
-
15
Streiger M, Fabbro D, del Barco M, Beltramino R, Bovero N, 1995. Congenital Chagas disease in the city of Santa Fe. Diagnosis and treatment. Medicina (B Aires) 55 :125–132.
-
16
Schijman AG, Altcheh J, Burgos JM, Biancardi M, Bisio M, Levin MJ, Freilij HJ, 2003. Aetiological treatment of congenital Chagas’ disease diagnosed and monitored by the polymerase chain reaction. J Antimicrob Chemother 52 :441–449.
-
17
Sánchez Negrette O, Mora MC, Basombrío MA, 2005. High prevalence of congenital Trypanosoma cruzi infection and family clustering in Salta, Argentina. Pediatrics 115 :668–672.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 272 | 219 | 15 |
| Full Text Views | 270 | 12 | 0 |
| PDF Downloads | 50 | 9 | 0 |