White AC Jr, 2005. Cryptosporidiosis (Cryptosporidium hominis, Cryptosporidium parvum, other species). Mandell GL, Ben-nett JE, Dolin R, eds. Principles and Practice of Infectious Diseases. Philadelphia: Elsevier Churchill Livingstone, 3215–3228.
Theodos CM, 1998. Innate and cell-mediated immune responses to Cryptosporidium parvum. Adv Parasitol 40 :87–119.
Gomez Morales MA, La Rosa G, Ludovisi A, Onori AM, Pozio E, 1999. Cytokine profile induced by Cryptosporidium antigen in peripheral blood mononuclear cells from immunocompetent and immunosuppressed persons with cryptosporidiosis. J Infect Dis 179 :967–973.
Gomez Morales MA, Mele R, Ludovisi A, Bruschi F, Tosini F, Rigano R, Pozio E, 2004. Cryptosporidium parvum-specific CD4 Th1 cells from sensitized donors responding to both fractionated and recombinant antigenic proteins. Infect Immun 72 :1306–1310.
White AC, Robinson P, Okhuysen PC, Lewis DE, Shahab I, Lahoti S, DuPont HL, Chappell CL, 2000. Interferon-gamma expression in jejunal biopsies in experimental human cryptosporidiosis correlates with prior sensitization and control of oocyst excretion. J Infect Dis 181 :701–709.
Strong WB, Gut J, Nelson RG, 2000. Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products. Infect Immun 68 :4117–4134.
Cevallos AM, Zhang X, Waldor MK, Jaison S, Zhou X, Tzipori S, Neutra MR, Ward HD, 2000. Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15. Infect Immun 68 :4108–4116.
Priest JW, Kwon JP, Arrowood MJ, Lammie PJ, 2000. Cloning of the immunodominant 17-kDa antigen from Cryptosporidium parvum. Mol Biochem Parasitol 106 :261–271.
Winter G, Gooley AA, Williams KL, Slade MB, 2000. Characterization of a major sporozoite surface glycoprotein of Cryptosporidium parvum. Funct Integr Genomics 1 :207–217.
Leav BA, Mackay MR, Anyanwu A, O’Connor RM, Cevallos AM, Kindra G, Rollins NC, Bennish ML, Nelson RG, Ward HD, 2002. Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South Africa. Infect Immun 70 :3881–3890.
McDonald AC, Mac Kenzie WR, Addiss DG, Gradus MS, Linke G, Zembrowski E, Hurd MR, Arrowood MJ, Lammie PJ, Priest JW, 2001. Cryptosporidium parvum-specific antibody responses among children residing in Milwaukee during the 1993 waterborne outbreak. J Infect Dis 183 :1373–1379.
Frost FJ, Roberts M, Kunde TR, Craun G, Tollestrup K, Harter L, Muller T, 2005. How clean must our drinking water be: the importance of protective immunity. J Infect Dis 191 :809–814.
Moss DM, Chappell CL, Okhuysen PC, DuPont HL, Arrowood MJ, Hightower AW, Lammie PJ, 1998. The antibody response to 27-, 17-, and 15-kDa Cryptosporidium antigens following experimental infection in humans. J Infect Dis 178 :827–833.
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Cryptosporidiosis is an important cause of diarrhea worldwide. In normal hosts, infection is self-limited and associated with seroconversion and partial immunity to reinfection. Immunity is associated with interferon gamma (IFNγ) production. Cryptosporidium surface proteins gp15 and gp40 are among the immunodominant proteins in terms of antibody responses. We asked the question of whether these antigens also stimulate production of IFNγ in patients who have serologic evidence of prior infection. Whole blood from seropositive donors was stimulated with recombinant gp15 and gp 40 from Cryptosporidium hominis and Cryptosporidium parvum or His-tag controls. C. hominis gp15 stimulated increased production of IFNγ. By contrast, there was no significant increase after stimulation with C. parvum gp15 or either gp40 preparation. IFNγ production in response to C. hominis gp15 was noted in both CD4+ and CD8+ cells. This highlights the potential for C. hominis gp15 as a vaccine candidate for human cryptosporidiosis.