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RefWorks
Reference Manager
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-
1
Muller HJ, 1932. Some genetic aspects of sex. Am Nat 66 :118–138.
-
2
Charlesworth B, Charlesworth D, 2000. The degeneration of Y chromosomes. Philos Trans R Soc Lond B Biol Sci 355 :1563– 1572.
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4
Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, 2003. The male-specific region of the human Y chromosome is a mosaic of discrete sequencie classes. Nature 423 :825–837.
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5
Bachtrog D, 2003. Accumulation of spock and worf, two novel non-LTR retrotransposons on the neo Y-chromosome of Drosophila miranda. Mol Biol Evol 20 :173–181.
-
6
Bachtrog D, 2003. Adaptation shapes pattern of genome evolution on sexual and asexual chromosomes in Drosophila. Nat Genet 34 :215–219.
-
7
Steinemann S, Steinemann M, 2000. Y chromosomes: born to be destroyed. Bioessays 27 :1076–1083.
-
8
Carvalho AB, Lazzaro BP, Clack AG, 2000. Y-chromosomal fertility factors kl-2 and kl-3 of Drosophila melanogaster encode dynein havy chain polypeptides. Proc Natl Acad Sci USA 98 :13225–13230.
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9
Carvalho AB, Dobo BA, Vibranovski MD, Clack AG, 2001. Identification of five new genes on the Y-chromosome of Drosophila melanogaster genome: how far can we go? Genetica 117 :227–237.
-
10
Steinemann M, Steinemann S, 1998. Enigma of Y-chromosome degeneration: neo-Y and neo-X chromosomes of Drosophila miranda a model for sex chromosome evolution. Genetica 102/103 :409–420.
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11
Krzywinski J, Nusskern DR, Kern MK, Besansky NJ, 2004. Isolation and Characterization of Y chromosome sequences from the African malaria mosquito Anopheles gambiae. Genetics 166 :1291–1302.
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12
Krzywinski J, Sangare D, Besansky NJ, 2005. Satellite DNA from the Y-chromosome of the malaria vector Anopheles gambiae. Genetics 169 :185–196.
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13
Clements AN, 1992. The Biology of Mosquitoes, Vol 1: Development, Nutrition and Reproduction. London: Chapmann and Hall.
-
14
Chomczynski P, Mackey K, Drews R, Wilfinger W, 1997. DNA-zol: a reagent for the rapid isolation of genomic DNA. Bio-techniques 22 :550–553.
-
15
Tabachnick WJ, 2003. Reflections on the Anopheles gambiae genome sequence, transgenic mosquitoes and the prospect for controlling malaria and other vector borne diseases. J Med Entomol 40 :597–606.
-
16
Catteruccia F, Godfray HCJ, Crisanti A, 2003. Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes. Science 299 :1225–1227.
-
17
Knols BGJ, Njiru BNN, Mathenge EM, Mukabana WR, Beier JC, Killeen GF, 2002. Malariasphere: a greenhouse-enclosed simulation of a natural Anopheles gambiae (Diptera: Culi-cidae) ecosystem in Western Kenya. Malar J 1 :19.
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18
Ferguson FM, John B, Ng’habi KR, Knols BGJ, 2005. Addressing the sex imbalance in knowledge of vector biology. Trends Ecol Evol 20 :202–209.
-
19
Knols BGJ, Njiru BNN, Mukabana RW, Mathenge EM, Killeen GF, 2003. Contained semi-field environments for ecological studies on transgenic African malaria vectors. Scott TW, Takken W, eds. Ecology of Transgenic Mosquitoes. Wageningen: Wageningen University and Research Centre, 99–106.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 540 | 500 | 281 |
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A New Robust Diagnostic Polymerase Chain Reaction for Determining the Mating Status of Female Anopheles gambiae Mosquitoes
Kija R. Ng’habi
Kija R. Ng’habi
Ifakara Health Research and Development Centre, Tanzania; Center for Vectorborne Diseases, University of California, Davis, California; Wageningen University and Research Centre, Wageningen, The Netherlands
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Ashley Horton
Ashley Horton
Ifakara Health Research and Development Centre, Tanzania; Center for Vectorborne Diseases, University of California, Davis, California; Wageningen University and Research Centre, Wageningen, The Netherlands
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Bart G. J. Knols
Bart G. J. Knols
Ifakara Health Research and Development Centre, Tanzania; Center for Vectorborne Diseases, University of California, Davis, California; Wageningen University and Research Centre, Wageningen, The Netherlands
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Gregory C. Lanzaro
Gregory C. Lanzaro
Ifakara Health Research and Development Centre, Tanzania; Center for Vectorborne Diseases, University of California, Davis, California; Wageningen University and Research Centre, Wageningen, The Netherlands
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The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome–specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome–specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Muller HJ, 1932. Some genetic aspects of sex. Am Nat 66 :118–138.
-
2
Charlesworth B, Charlesworth D, 2000. The degeneration of Y chromosomes. Philos Trans R Soc Lond B Biol Sci 355 :1563– 1572.
-
4
Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, 2003. The male-specific region of the human Y chromosome is a mosaic of discrete sequencie classes. Nature 423 :825–837.
-
5
Bachtrog D, 2003. Accumulation of spock and worf, two novel non-LTR retrotransposons on the neo Y-chromosome of Drosophila miranda. Mol Biol Evol 20 :173–181.
-
6
Bachtrog D, 2003. Adaptation shapes pattern of genome evolution on sexual and asexual chromosomes in Drosophila. Nat Genet 34 :215–219.
-
7
Steinemann S, Steinemann M, 2000. Y chromosomes: born to be destroyed. Bioessays 27 :1076–1083.
-
8
Carvalho AB, Lazzaro BP, Clack AG, 2000. Y-chromosomal fertility factors kl-2 and kl-3 of Drosophila melanogaster encode dynein havy chain polypeptides. Proc Natl Acad Sci USA 98 :13225–13230.
-
9
Carvalho AB, Dobo BA, Vibranovski MD, Clack AG, 2001. Identification of five new genes on the Y-chromosome of Drosophila melanogaster genome: how far can we go? Genetica 117 :227–237.
-
10
Steinemann M, Steinemann S, 1998. Enigma of Y-chromosome degeneration: neo-Y and neo-X chromosomes of Drosophila miranda a model for sex chromosome evolution. Genetica 102/103 :409–420.
-
11
Krzywinski J, Nusskern DR, Kern MK, Besansky NJ, 2004. Isolation and Characterization of Y chromosome sequences from the African malaria mosquito Anopheles gambiae. Genetics 166 :1291–1302.
-
12
Krzywinski J, Sangare D, Besansky NJ, 2005. Satellite DNA from the Y-chromosome of the malaria vector Anopheles gambiae. Genetics 169 :185–196.
-
13
Clements AN, 1992. The Biology of Mosquitoes, Vol 1: Development, Nutrition and Reproduction. London: Chapmann and Hall.
-
14
Chomczynski P, Mackey K, Drews R, Wilfinger W, 1997. DNA-zol: a reagent for the rapid isolation of genomic DNA. Bio-techniques 22 :550–553.
-
15
Tabachnick WJ, 2003. Reflections on the Anopheles gambiae genome sequence, transgenic mosquitoes and the prospect for controlling malaria and other vector borne diseases. J Med Entomol 40 :597–606.
-
16
Catteruccia F, Godfray HCJ, Crisanti A, 2003. Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes. Science 299 :1225–1227.
-
17
Knols BGJ, Njiru BNN, Mathenge EM, Mukabana WR, Beier JC, Killeen GF, 2002. Malariasphere: a greenhouse-enclosed simulation of a natural Anopheles gambiae (Diptera: Culi-cidae) ecosystem in Western Kenya. Malar J 1 :19.
-
18
Ferguson FM, John B, Ng’habi KR, Knols BGJ, 2005. Addressing the sex imbalance in knowledge of vector biology. Trends Ecol Evol 20 :202–209.
-
19
Knols BGJ, Njiru BNN, Mukabana RW, Mathenge EM, Killeen GF, 2003. Contained semi-field environments for ecological studies on transgenic African malaria vectors. Scott TW, Takken W, eds. Ecology of Transgenic Mosquitoes. Wageningen: Wageningen University and Research Centre, 99–106.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 540 | 500 | 281 |
| Full Text Views | 231 | 3 | 0 |
| PDF Downloads | 62 | 3 | 0 |