Author:
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- MSP-119 PCR/LDR-FMA determined allele specificity.
- Sensitivity and specificity of MSP-119 allele-specific PCR/LDR-FMA compared with sequencing.
- Pf parasite density by BS, RTQ-PCR, and PCR/LDR-FMA.
- Relative MSP-119 allele contribution to Pf infection can be determined with MSP-119 PCR/LDR-FMA.
- Prevalence and intensity of MSP-119 haplotypes in field isolates.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Blackman MJ, 2000. Proteases involved in erythrocyte invasion by the malaria parasite: function and potential as chemotherapeutic targets. Curr Drug Targets 1 :59–83.
-
2
Holder AA, Blackman MJ, Burghaus PA, Chappel JA, Ling IT, McCallum-Deighton N, Shai S, 1992. A malaria merozoite surface protein (MSP1)-structure, processing and function. Mem Inst Oswaldo Cruz 87 (Suppl 3):37–42.
-
3
Howell SA, Well I, Fleck SL, Kettleborough C, Collins CR, Blackman MJ, 2003. A single malaria merozoite serine protease mediates shedding of multiple surface proteins by juxtamembrane cleavage. J Biol Chem 278 :23890–23898.
-
4
Udhayakumar V, Anyona D, Kariuki S, Shi YP, Bloland PB, Branch OH, Weiss W, Nahlen BL, Kaslow DC, Lal AA, 1995. Identification of T and B cell epitopes recognized by humans in the C-terminal 42-kDa domain of the Plasmodium falciparum merozoite surface protein (MSP)-1. J Immunol 154 :6022–6030.
-
5
Conway DJ, Fanello C, Lloyd JM, Al-Joubori BM, Baloch AH, Somanath SD, Roper C, Oduola AM, Mulder B, Povoa MM, Singh B, Thomas AW, 2000. Origin of Plasmodium falciparum malaria is traced by mitochondrial DNA. Mol Biochem Parasitol 111 :163–171.
-
6
Lee EA, Flanagan KL, Odhiambo K, Reece WH, Potter C, Bailey R, Marsh K, Pinder M, Hill AV, Plebanski M, 2001. Identification of frequently recognized dimorphic T-cell epitopes in plasmodium falciparum merozoite surface protein-1 in West and East Africans: lack of correlation of immune recognition and allelic prevalence. Am J Trop Med Hyg 64 :194–203.
-
7
Kaneko O, Kimura M, Kawamoto F, Ferreira MU, Tanabe K, 1997. Plasmodium falciparum: allelic variation in the merozoite surface protein 1 gene in wild isolates from southern Vietnam. Exp Parasitol 86 :45–57.
-
8
Kang Y, Long CA, 1995. Sequence heterogeneity of the C-terminal, Cys-rich region of the merozoite surface protein-1 (MSP-1) in field samples of Plasmodium falciparum. Mol Biochem Parasitol 73 :103–110.
-
9
Branch OH, Udhayakumar V, Hightower AW, Oloo AJ, Hawley WA, Nahlen BL, Bloland PB, Kaslow DC, Lal AA, 1998. A longitudinal investigation of IgG and IgM antibody responses to the merozoite surface protein-1 19-kiloDalton domain of Plasmodium falciparum in pregnant women and infants: associations with febrile illness, parasitemia, and anemia. Am J Trop Med Hyg 58 :211–219.
-
10
Dodoo D, Theander TG, Kurtzhals JA, Koram K, Riley E, Akanmori BD, Nkrumah FK, Hviid L, 1999. Levels of antibody to conserved parts of Plasmodium falciparum merozoite surface protein 1 in Ghanaian children are not associated with protection from clinical malaria. Infect Immun 67 :2131–2137.
-
11
Egan AF, Morris J, Barnish G, Allen S, Greenwood BM, Kaslow DC, Holder AA, Riley EM, 1996. Clinical immunity to Plasmodium falciparum malaria is associated with serum antibodies to the 19-kDa C-terminal fragment of the merozoite surface antigen, PfMSP-1. J Infect Dis 173 :765–769.
-
12
Riley EM, Allen SJ, Wheeler JG, Blackman MJ, Bennett S, Takacs B, Schonfeld HJ, Holder AA, Greenwood BM, 1992. Naturally acquired cellular and humoral immune responses to the major merozoite surface antigen (PfMSP1) of Plasmodium falciparum are associated with reduced malaria morbidity. Parasite Immunol 14 :321–337.
-
13
Singh S, Miura K, Zhou H, Muratova O, Keegan B, Miles A, Martin LB, Saul AJ, Miller LH, Long CA, 2006. Immunity to recombinant plasmodium falciparum merozoite surface protein 1 (MSP1): protection in Aotus nancymai monkeys strongly correlates with anti-MSP1 antibody titer and in vitro parasite-inhibitory activity. Infect Immun 74 :4573–4580.
-
14
Hunt P, Fawcett R, Carter R, Walliker D, 2005. Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications. Mol Biochem Parasitol 143 :173–182.
-
15
Takala SL, Smith DL, Stine OC, Coulibaly D, Thera MA, Doumbo OK, Plowe CV, 2006. A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa. Malar J 5 :31.
-
16
Bejon P, Andrews L, Hunt-Cooke A, Sanderson F, Gilbert SC, Hill AV, 2006. Thick blood film examination for Plasmodium falciparum malaria has reduced sensitivity and underestimates parasite density. Malar J 5 :104.
-
17
Malhotra I, Dent A, Mungai P, Muchiri E, King CL, 2005. Real-time quantitative PCR for determining the burden of Plasmodium falciparum parasites during pregnancy and infancy. J Clin Microbiol 43 :3630–3635.
-
18
Hermsen CC, Telgt DS, Linders EH, van de Locht LA, Eling WM, Mensink EJ, Sauerwein RW, 2001. Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR. Mol Biochem Parasitol 118 :247–251.
-
19
McNamara DT, Kasehagen LJ, Grimberg BT, Cole-Tobian J, Collins WE, Zimmerman PA, 2006. Diagnosing infection levels of four human malaria parasite species by a polymerase chain reaction/ligase detection reaction fluorescent microsphere-based assay. Am J Trop Med Hyg 74 :413–421.
-
20
Carnevale EP, Kouri D, Dare JT, McNamara DT, Mueller I, Zimmerman PA, 2006. A multiplex ligase detection reaction-fluorescent microsphere assay (LDR-FMA) for simultaneous diagnosis of single nucleotide polymorphisms associated with Plasmodium falciparum drug resistance. J Clin Microbiol 45 :752–761.
-
21
Qari SH, Shi YP, Goldman IF, Nahlen BL, Tibayrenc M, Lal AA, 1998. Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen. Mol Biochem Parasitol 92 :241–252.
-
22
Withers MR, McKinney D, Ogutu BR, Waitumbi JN, Milman JB, Apollo OJ, Allen OG, Tucker K, Soisson LA, Diggs C, Leach A, Wittes J, Dubovsky F, Stewart VA, Remich SA, Cohen J, Ballou WR, Holland CA, Lyon JA, Angov E, Stoute JA, Martin SK, Heppner DG, 2006. Safety and reactogenicity of an MSP-1 malaria vaccine candidate: a randomized phase Ib dose-escalation trial in Kenyan children. PLoS Clin Trials 1 :e32.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 8 | 8 | 2 |
| Full Text Views | 72 | 25 | 0 |
| PDF Downloads | 7 | 0 | 0 |
A Polymerase Chain Reaction/Ligase Detection Reaction–Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
Arlene E. Dent
Arlene E. Dent
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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Christopher T. Yohn
Christopher T. Yohn
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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Peter A. Zimmerman
Peter A. Zimmerman
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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John Vulule
John Vulule
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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James W. Kazura
James W. Kazura
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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Ann M. Moormann
Ann M. Moormann
Center for Global Health and Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio; Kenya Medical Research Institute, Center for Vector Control and Biology Research, Kisumu, Kenya
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The merozoite surface protein-1 (MSP-1) is a blood stage antigen currently being tested as a vaccine against Plasmodium falciparum malaria. Determining the MSP-119 haplotype(s) present during infection is essential for assessments of MSP-1 vaccine efficacy and studies of protective immunity in human populations. The C-terminal fragment (MSP-119) has four predominant haplotypes based on point mutations resulting in non-synonymous amino acid changes: E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type). Current techniques using direct DNA sequencing are laborious and expensive. We present an MSP-119 allele-specific polymerase chain reaction (PCR)/ligase detection reaction–fluorescent microsphere assay (LDR-FMA) that allows simultaneous detection of the four predominant MSP-119 haplotypes with a sensitivity and specificity comparable with other molecular methods and a semi-quantitative determination of haplotype contribution in mixed infections. Application of this method is an inexpensive, accurate, and high-throughput alternative to distinguish the predominant MSP-119 haplotypes in epidemiologic studies.
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- MSP-119 PCR/LDR-FMA determined allele specificity.
- Sensitivity and specificity of MSP-119 allele-specific PCR/LDR-FMA compared with sequencing.
- Pf parasite density by BS, RTQ-PCR, and PCR/LDR-FMA.
- Relative MSP-119 allele contribution to Pf infection can be determined with MSP-119 PCR/LDR-FMA.
- Prevalence and intensity of MSP-119 haplotypes in field isolates.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Blackman MJ, 2000. Proteases involved in erythrocyte invasion by the malaria parasite: function and potential as chemotherapeutic targets. Curr Drug Targets 1 :59–83.
-
2
Holder AA, Blackman MJ, Burghaus PA, Chappel JA, Ling IT, McCallum-Deighton N, Shai S, 1992. A malaria merozoite surface protein (MSP1)-structure, processing and function. Mem Inst Oswaldo Cruz 87 (Suppl 3):37–42.
-
3
Howell SA, Well I, Fleck SL, Kettleborough C, Collins CR, Blackman MJ, 2003. A single malaria merozoite serine protease mediates shedding of multiple surface proteins by juxtamembrane cleavage. J Biol Chem 278 :23890–23898.
-
4
Udhayakumar V, Anyona D, Kariuki S, Shi YP, Bloland PB, Branch OH, Weiss W, Nahlen BL, Kaslow DC, Lal AA, 1995. Identification of T and B cell epitopes recognized by humans in the C-terminal 42-kDa domain of the Plasmodium falciparum merozoite surface protein (MSP)-1. J Immunol 154 :6022–6030.
-
5
Conway DJ, Fanello C, Lloyd JM, Al-Joubori BM, Baloch AH, Somanath SD, Roper C, Oduola AM, Mulder B, Povoa MM, Singh B, Thomas AW, 2000. Origin of Plasmodium falciparum malaria is traced by mitochondrial DNA. Mol Biochem Parasitol 111 :163–171.
-
6
Lee EA, Flanagan KL, Odhiambo K, Reece WH, Potter C, Bailey R, Marsh K, Pinder M, Hill AV, Plebanski M, 2001. Identification of frequently recognized dimorphic T-cell epitopes in plasmodium falciparum merozoite surface protein-1 in West and East Africans: lack of correlation of immune recognition and allelic prevalence. Am J Trop Med Hyg 64 :194–203.
-
7
Kaneko O, Kimura M, Kawamoto F, Ferreira MU, Tanabe K, 1997. Plasmodium falciparum: allelic variation in the merozoite surface protein 1 gene in wild isolates from southern Vietnam. Exp Parasitol 86 :45–57.
-
8
Kang Y, Long CA, 1995. Sequence heterogeneity of the C-terminal, Cys-rich region of the merozoite surface protein-1 (MSP-1) in field samples of Plasmodium falciparum. Mol Biochem Parasitol 73 :103–110.
-
9
Branch OH, Udhayakumar V, Hightower AW, Oloo AJ, Hawley WA, Nahlen BL, Bloland PB, Kaslow DC, Lal AA, 1998. A longitudinal investigation of IgG and IgM antibody responses to the merozoite surface protein-1 19-kiloDalton domain of Plasmodium falciparum in pregnant women and infants: associations with febrile illness, parasitemia, and anemia. Am J Trop Med Hyg 58 :211–219.
-
10
Dodoo D, Theander TG, Kurtzhals JA, Koram K, Riley E, Akanmori BD, Nkrumah FK, Hviid L, 1999. Levels of antibody to conserved parts of Plasmodium falciparum merozoite surface protein 1 in Ghanaian children are not associated with protection from clinical malaria. Infect Immun 67 :2131–2137.
-
11
Egan AF, Morris J, Barnish G, Allen S, Greenwood BM, Kaslow DC, Holder AA, Riley EM, 1996. Clinical immunity to Plasmodium falciparum malaria is associated with serum antibodies to the 19-kDa C-terminal fragment of the merozoite surface antigen, PfMSP-1. J Infect Dis 173 :765–769.
-
12
Riley EM, Allen SJ, Wheeler JG, Blackman MJ, Bennett S, Takacs B, Schonfeld HJ, Holder AA, Greenwood BM, 1992. Naturally acquired cellular and humoral immune responses to the major merozoite surface antigen (PfMSP1) of Plasmodium falciparum are associated with reduced malaria morbidity. Parasite Immunol 14 :321–337.
-
13
Singh S, Miura K, Zhou H, Muratova O, Keegan B, Miles A, Martin LB, Saul AJ, Miller LH, Long CA, 2006. Immunity to recombinant plasmodium falciparum merozoite surface protein 1 (MSP1): protection in Aotus nancymai monkeys strongly correlates with anti-MSP1 antibody titer and in vitro parasite-inhibitory activity. Infect Immun 74 :4573–4580.
-
14
Hunt P, Fawcett R, Carter R, Walliker D, 2005. Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications. Mol Biochem Parasitol 143 :173–182.
-
15
Takala SL, Smith DL, Stine OC, Coulibaly D, Thera MA, Doumbo OK, Plowe CV, 2006. A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa. Malar J 5 :31.
-
16
Bejon P, Andrews L, Hunt-Cooke A, Sanderson F, Gilbert SC, Hill AV, 2006. Thick blood film examination for Plasmodium falciparum malaria has reduced sensitivity and underestimates parasite density. Malar J 5 :104.
-
17
Malhotra I, Dent A, Mungai P, Muchiri E, King CL, 2005. Real-time quantitative PCR for determining the burden of Plasmodium falciparum parasites during pregnancy and infancy. J Clin Microbiol 43 :3630–3635.
-
18
Hermsen CC, Telgt DS, Linders EH, van de Locht LA, Eling WM, Mensink EJ, Sauerwein RW, 2001. Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR. Mol Biochem Parasitol 118 :247–251.
-
19
McNamara DT, Kasehagen LJ, Grimberg BT, Cole-Tobian J, Collins WE, Zimmerman PA, 2006. Diagnosing infection levels of four human malaria parasite species by a polymerase chain reaction/ligase detection reaction fluorescent microsphere-based assay. Am J Trop Med Hyg 74 :413–421.
-
20
Carnevale EP, Kouri D, Dare JT, McNamara DT, Mueller I, Zimmerman PA, 2006. A multiplex ligase detection reaction-fluorescent microsphere assay (LDR-FMA) for simultaneous diagnosis of single nucleotide polymorphisms associated with Plasmodium falciparum drug resistance. J Clin Microbiol 45 :752–761.
-
21
Qari SH, Shi YP, Goldman IF, Nahlen BL, Tibayrenc M, Lal AA, 1998. Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen. Mol Biochem Parasitol 92 :241–252.
-
22
Withers MR, McKinney D, Ogutu BR, Waitumbi JN, Milman JB, Apollo OJ, Allen OG, Tucker K, Soisson LA, Diggs C, Leach A, Wittes J, Dubovsky F, Stewart VA, Remich SA, Cohen J, Ballou WR, Holland CA, Lyon JA, Angov E, Stoute JA, Martin SK, Heppner DG, 2006. Safety and reactogenicity of an MSP-1 malaria vaccine candidate: a randomized phase Ib dose-escalation trial in Kenyan children. PLoS Clin Trials 1 :e32.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 8 | 8 | 2 |
| Full Text Views | 72 | 25 | 0 |
| PDF Downloads | 7 | 0 | 0 |