ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
World Health Organization, 2005. World Malaria Report. Roll Back Malaria. New York: United Nations Children Fund.
-
2
Instituto Nacional de Salud, Ministerio de Salud - INS, 2002. Boletín Epidemiológico Semanal. Sistema Nacional de Vigilancia en Salud Pública-SIVIGILA. Semana Epidemiológica No. 52 Diciembre 22–28. Bogota, Colombia: Ministerio de Salud.
-
3
Olano VA, Brochero HL, Saénz R, Quiñones ML, Molina JA, 2001. Mapas preliminares de la distribución de especies de Anopheles vectores de malaria en Colombia. Biomedica (Bogota) 21 :402–408.
-
4
Ruiz F, Quiñones ML, Erazo H, Calle D, Alzate J, Linton YM, 2005. Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from Southern Colombia. Mem Inst Oswaldo Cruz 100 :155–160.
-
5
Li C, Wilkerson R, 2005. Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes. Mem Inst Oswaldo Cruz 100 :495–500.
-
6
Lounibos LP, Wilkerson RC, Conn JE, Hribar LJ, Fritz GN, Danoff-Burg JA, 1998. Morphological, molecular, and chromosomal discrimination of cryptic Anopheles (Nyssorhynchus) (Diptera:Culicidae) from South America. J Med Entomol 35 :830–838.
-
7
Marrelli MT, Floeter-Winter LM, Malafronte RS, Tadei WP, Lourenco-de-Oliveira R, Flores-Mendoza C, Marinotti O, 2005. Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences. Med Vet Entomol 19 :208–218.
-
8
Krzywinski J, Besansky NJ, 2003. Molecular systematics of Anopheles: from subgenera to subpopulations. Annu Rev Entomol 48 :111–139.
-
9
Beebe NW, Cooper RD, Foley DH, Ellis JT, 2000. Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms. Heredity 8 :244–253.
-
10
Fairley T, Kilpatrick C, Conn JE, 2005. Intragenomic heterogeneity of internal transcribed spacer rDNA in neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae). J Med Entomol 45 :795–800.
-
11
Linton YM, Samanidou-Voyadjoglou A, Harbach RE, 2002. Ribosomal ITS2 sequence data for Anopheles maculipennis and An. messeae in northern Greece, with a critical assessment of previously published sequences. Insect Mol Biol 11 :379–383.
-
12
Beebe NW, Ellis JT, Cooper RD, Saul A, 1999. DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes. Insect Mol Biol 8 :381–390.
-
13
Cohuet A, Simard F, Toto J, Kengne P, Coetzee M, Fontenille D, 2003. Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species. Am J Trop Med Hyg 69 :200–205.
-
14
Fritz G, Engman S, Rodriguez R, Wilkerson R, 2004. Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. Strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus). J Med Entomol 41 :1111–1115.
-
15
Garros C, Koekemoer LL, Coetzee M, Coosemans M, Manguin S, 2004. A single multiplex assay to identify major malaria vectors within the african Anopheles funestus and the oriental An. minimus groups. Am J Trop Med Hyg 70 :583–590.
-
16
Koekemoer L, Kamau L, Hunt R, Coetzee M, 2002. A cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group. Am J Trop Med Hyg 66 :804–811.
-
17
Beebe NW, Saul A, 1995. Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction–restriction fragment length polymorphism analysis. Am J Trop Med Hyg 53 :478–481.
-
18
Favia G, della Torre A, Bagayoko M, Lanfrancotti A, Sagnon N, Toure YT, Coluzzi M, 1997. Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6 :377–383.
-
19
Fanello C, Santolamazza F, della Torre A, 2002. Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP. Med Vet Entomol 16 :461–464.
-
20
Garros C, Koekemoer LL, Kamau L, Awolola TS, Van Bortel W, Coetzee M, Coosemans M, Manguin S, 2004. Restriction fragment length polymorphism method for the identification of major African and Asian malaria vectors within the Anopheles funestus and An. minimus groups. Am J Trop Med Hyg 70 :260–265.
-
21
Goswami G, Raghavendra K, Nanda N, Gakhar SK, Subbarao SK, 2005. PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae). Acta Trop 95 :92–99.
-
22
Sierra DM, Vélez ID, Linton YM, 2004. Malaria vector Anopheles (Nyssorhynchus) nuneztovari comprises one genetic species in Colombia based on homogeneity of nuclear ITS2 rDNA. J Med Entomol 41 :302–307.
-
23
Dirección Seccional de Salud Antioquia, 2006. Eventos de Salud Pública. Enfermedades transmitidas por Vectores. [cited October 5, 2006]. Available from http://www.dssa.gov.co/htm/inciden.htm
-
24
Faran M, 1980. A Revision of the Albimanus Section of the Subgenus Nyssorhynchus of the Anopheles. Mosquitoes studies (Diptera: Culicidae). Contributions of the American Entomological Institute XXXIV. Volume 15. No. 7. Gainesville, FL: American Entomological Institute.
-
25
Faran M, Linthicum L, 1981. A handbook of the Amazonian species of Anopheles (Nyssorhynchus) (Diptera: Culicidae). Mosquito Systematics. 13 :1–81.
-
26
Suarez MF, Quiñones ML, Robayo MA, 1988. Clave Grafica para la Determinación Taxonómica de los Anofelinos de Colombia. Bogota, Colombia: Ministerio de Salud, Dirección de Campañas Directas, División Tecnica, Programa Antimalárico.
-
27
Wilkerson RC, Strickman D, 1993. Clave Ilustrada para la Identificación de las Hembras de Mosquitos de México y Centroamérica. Mexico City, Mexico: Secretaria de Salud, Subsecretaria de Coordinación y Desarrollo.
-
28
Ashburner M, 1989. Drosophila: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, protocol 48, 47–48.
-
29
Vincze T, Posfai J, Roberts RJ, 2003. NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res 31 :3688–3691.
-
30
Quinones ML, Ruiz F, Calle DA, Harbach RE, Erazo HF, Linton YM, 2006. Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in Southern Colombia. Mem Inst Oswaldo Cruz 101 :617–623.
-
31
da Silva-Vasconcelos A, Kato MY, Mourao EN, de Souza RT, Lacerda RN, Sibajev A, Tsouris P, Povoa MM, Momen H, Rosa-Freitas MG, 2002. Biting indices, host-seeking activity and natural infection rates of anopheline species in Boa Vista, Roraima, Brazil from 1996 to 1998. Mem Inst Oswaldo Cruz 97 :151–161.
-
32
Povoa MM, de Souza RT, Lacerda RN, Rosa ES, Galiza D, de Souza JR, Wirtz RA, Schlichting CD, Conn JE, 2006. The importance of Anopheles albitarsis E and An. darlingi in human malaria transmission in Boa Vista, state of Roraima, Brazil. Mem Inst Oswaldo Cruz 101 :163–168.
-
33
Rubio-Palis Y, Zimmerman RH, 1997. Ecoregional classification of malaria vectors in the neotropics. J Med Entomol 34 :499–510.
-
34
Delgado N, Rubio-Palis Y, 1992. Morphometric characterization of the malaria vector Anopheles nuñeztovari (Diptera: Culicidae) from western Venezuela. Mosquito Systematics. 24 :231–241.
-
35
Marrelli MT, Sallum MA, Marinotti O, 2006. The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy: a critical review. Mem Inst Oswaldo Cruz 101 :817–832.
-
36
Li C, Wilkerson RC, 2007. Intragenomic rDNA ITS2 variation in the neotropical Anopheles (Nyssorhynchus) albitarsis complex (Diptera: Culicidae). J Hered 98 :51–59.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 902 | 832 | 326 |
| Full Text Views | 161 | 11 | 1 |
| PDF Downloads | 60 | 12 | 2 |
Discrimination of Seven Anopheles Species from San Pedro de Urabá, Antioquia, Colombia, by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of Its Sequences
Mario A. Zapata
Mario A. Zapata
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Mario A. Zapata in
Current site
Google Scholar
PubMed
Search for other papers by Mario A. Zapata in
Current site
Google Scholar
PubMed
Astrid V. Cienfuegos
Astrid V. Cienfuegos
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Astrid V. Cienfuegos in
Current site
Google Scholar
PubMed
Search for other papers by Astrid V. Cienfuegos in
Current site
Google Scholar
PubMed
Oscar I. Quirós
Oscar I. Quirós
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Oscar I. Quirós in
Current site
Google Scholar
PubMed
Search for other papers by Oscar I. Quirós in
Current site
Google Scholar
PubMed
Martha L. Quiñones
Martha L. Quiñones
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Martha L. Quiñones in
Current site
Google Scholar
PubMed
Search for other papers by Martha L. Quiñones in
Current site
Google Scholar
PubMed
Shirley Luckhart
Shirley Luckhart
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Shirley Luckhart in
Current site
Google Scholar
PubMed
Search for other papers by Shirley Luckhart in
Current site
Google Scholar
PubMed
Margarita M. Correa
Margarita M. Correa
Grupo de Microbiología Molecular, Escuela de Microbiología, and Programa para el Estudio y Control de Enfermedades Tropicales, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Medical Microbiology and Immnunology, School of Medicine, University of California, Davis, California
Search for other papers by Margarita M. Correa in
Current site
Google Scholar
PubMed
Search for other papers by Margarita M. Correa in
Current site
Google Scholar
PubMed
Accurate identification of anopheline species is essential for vector incrimination and implementation of appropriate control strategies. Several anopheline species are considered important malaria vectors in Colombia; however, species determination is complicated by cryptic morphology and intra-individual variation. We describe polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer 2 (ITS2) sequences for differentiation of seven Anopheles species collected in a locality in Antioquia, Colombia, with high levels of malaria transmission. Each of these seven species can be identified by unique AluI PCR-RFLP restriction patterns. Comparisons of morphologic identification with molecular identification of voucher specimens confirmed species designation for 886 wild-caught anophelines. This new method can be used as a diagnostic tool for discrimination of anopheline species of medical importance in this region, some of which have overlapping morphologic characters and for conducting complementary studies where rapid and accurate identification of large numbers of specimens is needed.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
World Health Organization, 2005. World Malaria Report. Roll Back Malaria. New York: United Nations Children Fund.
-
2
Instituto Nacional de Salud, Ministerio de Salud - INS, 2002. Boletín Epidemiológico Semanal. Sistema Nacional de Vigilancia en Salud Pública-SIVIGILA. Semana Epidemiológica No. 52 Diciembre 22–28. Bogota, Colombia: Ministerio de Salud.
-
3
Olano VA, Brochero HL, Saénz R, Quiñones ML, Molina JA, 2001. Mapas preliminares de la distribución de especies de Anopheles vectores de malaria en Colombia. Biomedica (Bogota) 21 :402–408.
-
4
Ruiz F, Quiñones ML, Erazo H, Calle D, Alzate J, Linton YM, 2005. Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from Southern Colombia. Mem Inst Oswaldo Cruz 100 :155–160.
-
5
Li C, Wilkerson R, 2005. Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes. Mem Inst Oswaldo Cruz 100 :495–500.
-
6
Lounibos LP, Wilkerson RC, Conn JE, Hribar LJ, Fritz GN, Danoff-Burg JA, 1998. Morphological, molecular, and chromosomal discrimination of cryptic Anopheles (Nyssorhynchus) (Diptera:Culicidae) from South America. J Med Entomol 35 :830–838.
-
7
Marrelli MT, Floeter-Winter LM, Malafronte RS, Tadei WP, Lourenco-de-Oliveira R, Flores-Mendoza C, Marinotti O, 2005. Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences. Med Vet Entomol 19 :208–218.
-
8
Krzywinski J, Besansky NJ, 2003. Molecular systematics of Anopheles: from subgenera to subpopulations. Annu Rev Entomol 48 :111–139.
-
9
Beebe NW, Cooper RD, Foley DH, Ellis JT, 2000. Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms. Heredity 8 :244–253.
-
10
Fairley T, Kilpatrick C, Conn JE, 2005. Intragenomic heterogeneity of internal transcribed spacer rDNA in neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae). J Med Entomol 45 :795–800.
-
11
Linton YM, Samanidou-Voyadjoglou A, Harbach RE, 2002. Ribosomal ITS2 sequence data for Anopheles maculipennis and An. messeae in northern Greece, with a critical assessment of previously published sequences. Insect Mol Biol 11 :379–383.
-
12
Beebe NW, Ellis JT, Cooper RD, Saul A, 1999. DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes. Insect Mol Biol 8 :381–390.
-
13
Cohuet A, Simard F, Toto J, Kengne P, Coetzee M, Fontenille D, 2003. Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species. Am J Trop Med Hyg 69 :200–205.
-
14
Fritz G, Engman S, Rodriguez R, Wilkerson R, 2004. Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. Strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus). J Med Entomol 41 :1111–1115.
-
15
Garros C, Koekemoer LL, Coetzee M, Coosemans M, Manguin S, 2004. A single multiplex assay to identify major malaria vectors within the african Anopheles funestus and the oriental An. minimus groups. Am J Trop Med Hyg 70 :583–590.
-
16
Koekemoer L, Kamau L, Hunt R, Coetzee M, 2002. A cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group. Am J Trop Med Hyg 66 :804–811.
-
17
Beebe NW, Saul A, 1995. Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction–restriction fragment length polymorphism analysis. Am J Trop Med Hyg 53 :478–481.
-
18
Favia G, della Torre A, Bagayoko M, Lanfrancotti A, Sagnon N, Toure YT, Coluzzi M, 1997. Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6 :377–383.
-
19
Fanello C, Santolamazza F, della Torre A, 2002. Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP. Med Vet Entomol 16 :461–464.
-
20
Garros C, Koekemoer LL, Kamau L, Awolola TS, Van Bortel W, Coetzee M, Coosemans M, Manguin S, 2004. Restriction fragment length polymorphism method for the identification of major African and Asian malaria vectors within the Anopheles funestus and An. minimus groups. Am J Trop Med Hyg 70 :260–265.
-
21
Goswami G, Raghavendra K, Nanda N, Gakhar SK, Subbarao SK, 2005. PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae). Acta Trop 95 :92–99.
-
22
Sierra DM, Vélez ID, Linton YM, 2004. Malaria vector Anopheles (Nyssorhynchus) nuneztovari comprises one genetic species in Colombia based on homogeneity of nuclear ITS2 rDNA. J Med Entomol 41 :302–307.
-
23
Dirección Seccional de Salud Antioquia, 2006. Eventos de Salud Pública. Enfermedades transmitidas por Vectores. [cited October 5, 2006]. Available from http://www.dssa.gov.co/htm/inciden.htm
-
24
Faran M, 1980. A Revision of the Albimanus Section of the Subgenus Nyssorhynchus of the Anopheles. Mosquitoes studies (Diptera: Culicidae). Contributions of the American Entomological Institute XXXIV. Volume 15. No. 7. Gainesville, FL: American Entomological Institute.
-
25
Faran M, Linthicum L, 1981. A handbook of the Amazonian species of Anopheles (Nyssorhynchus) (Diptera: Culicidae). Mosquito Systematics. 13 :1–81.
-
26
Suarez MF, Quiñones ML, Robayo MA, 1988. Clave Grafica para la Determinación Taxonómica de los Anofelinos de Colombia. Bogota, Colombia: Ministerio de Salud, Dirección de Campañas Directas, División Tecnica, Programa Antimalárico.
-
27
Wilkerson RC, Strickman D, 1993. Clave Ilustrada para la Identificación de las Hembras de Mosquitos de México y Centroamérica. Mexico City, Mexico: Secretaria de Salud, Subsecretaria de Coordinación y Desarrollo.
-
28
Ashburner M, 1989. Drosophila: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, protocol 48, 47–48.
-
29
Vincze T, Posfai J, Roberts RJ, 2003. NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res 31 :3688–3691.
-
30
Quinones ML, Ruiz F, Calle DA, Harbach RE, Erazo HF, Linton YM, 2006. Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in Southern Colombia. Mem Inst Oswaldo Cruz 101 :617–623.
-
31
da Silva-Vasconcelos A, Kato MY, Mourao EN, de Souza RT, Lacerda RN, Sibajev A, Tsouris P, Povoa MM, Momen H, Rosa-Freitas MG, 2002. Biting indices, host-seeking activity and natural infection rates of anopheline species in Boa Vista, Roraima, Brazil from 1996 to 1998. Mem Inst Oswaldo Cruz 97 :151–161.
-
32
Povoa MM, de Souza RT, Lacerda RN, Rosa ES, Galiza D, de Souza JR, Wirtz RA, Schlichting CD, Conn JE, 2006. The importance of Anopheles albitarsis E and An. darlingi in human malaria transmission in Boa Vista, state of Roraima, Brazil. Mem Inst Oswaldo Cruz 101 :163–168.
-
33
Rubio-Palis Y, Zimmerman RH, 1997. Ecoregional classification of malaria vectors in the neotropics. J Med Entomol 34 :499–510.
-
34
Delgado N, Rubio-Palis Y, 1992. Morphometric characterization of the malaria vector Anopheles nuñeztovari (Diptera: Culicidae) from western Venezuela. Mosquito Systematics. 24 :231–241.
-
35
Marrelli MT, Sallum MA, Marinotti O, 2006. The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy: a critical review. Mem Inst Oswaldo Cruz 101 :817–832.
-
36
Li C, Wilkerson RC, 2007. Intragenomic rDNA ITS2 variation in the neotropical Anopheles (Nyssorhynchus) albitarsis complex (Diptera: Culicidae). J Hered 98 :51–59.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 902 | 832 | 326 |
| Full Text Views | 161 | 11 | 1 |
| PDF Downloads | 60 | 12 | 2 |