EVALUATION OF FAS2-ELISA FOR THE SEROLOGICAL DETECTION OF FASCIOLA HEPATICA INFECTION IN HUMANS

JOSE R. ESPINOZA Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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VICENTE MACO Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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LUIS MARCOS Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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SANDRA SAEZ Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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VICTOR NEYRA Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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ANGELICA TERASHIMA Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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FRINE SAMALVIDES Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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EDUARDO GOTUZZO Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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ELIZABETH CHAVARRY Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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MARIA CECILIA HUAMAN Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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M. DOLORES BARGUES Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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M. ADELA VALERO Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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SANTIAGO MAS-COMA Unit of Molecular Biotechnology, Laboratorios de Investigación y Desarrollo (LID), Facultad de Ciencias y Filosofia; Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru; Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain

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The performance of Fas2-ELISA for the diagnosis of Fasciola hepatica infection in children living in areas of high endemicity for fascioliasis in the Peruvian Andes is analyzed. Fas2-ELISA is based on the detection of circulating IgG antibodies elicited in infected individuals against a F. hepatica antigen termed Fas2. The study was conducted in three Andean localities, Huertas-Julcan in Junin, Asillo in Puno, and Cajamarca, with a total population of 634 children in an age range 1 to 16 years old. Child fascioliasis prevalence was 21.1% in Huertas-Julcan, 25.4% in Asillo, and 24% in Cajamarca, estimated by coprological inspection. The seroprevalence of F. hepatica infection, determined by Fas2-ELISA, was 27.8% in Huertas-Julcan, 44.6% in Asillo, and 29.1% in Cajamarca. The overall sensitivity of Fas2-ELISA was 92.4%, the specificity 83.6%, and the negative predictive value 97.2%. No association between OD450 Fas2-ELISA and infection intensity measured by egg counting was observed. Results show that Fas2-ELISA is a highly sensitive immunodiagnostic test for the detection of F. hepatica infection in children living in human fascioliasis endemic areas.

Author Notes

Reprint requests: Jose R. Espinoza, Unit of Molecular Biotechnology, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima 100, Peru, Telephone: + 511 319 0000 ext. 2502, Fax: + 511 319 0024, E-mail: jrespinoza@upch.edu.pe.
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