Author:
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- Search for repeated sequences and primers specific for S. haematobium.
- Structure, abundance, and arrangement of the Sh110 repeated sequence.
- Structure, abundance, and arrangement of the Sh110/Sm-Sl PCR product.
- Differential identification of S. haematobium by PCR: detection sensitivity and specificity.
- Detection of S. haematobiumāinfected snails by PCR.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
WHO, 2002. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. World Health Organ Tech Rep Ser 912 :2ā3.
-
2
Loker ES, 1983. A comparative study of the life-histories of mammalian schistosomes. Parasitology 87 :343ā369.
-
3
Rollinson D, Stothard JR, Southgate VR, 2001. Interactions between intermediate snail hosts of the genus Bulinus and schistosomes of the Schistosoma haematobium group. Parasitology 123 (Suppl):S245āS260.
-
4
Chipaux JP, Boulanger D, Bremond P, Campagne G, Vera C, Sellin B, 1997. The WHO Collaborating Centre for Research and Control of schistosomiasis at Niamey, Niger. Mem Inst Oswaldo Cruz 92 :725ā728.
-
5
Woolhouse ME, Chandiwana SK, 1989. Spatial and temporal heterogeneity in the population dynamics of Bulinus globosus and Biomphalaria pfeifferi and in the epidemiology of their infection with schistosomes. Parasitology 98 :21ā34.
-
6
Woolhouse MEJ, Chandiwana SK, 1990. The epidemiology of schistosome infections of snails: taking the theory into the field. Parasitol Today 6 :65ā70.
-
7
Christensen NO, Mutani A, Frandsen F, 1983. A review of the biology and transmission ecology of African bovine species of the genus Schistosoma.Z Parasitenkd 69 :551ā570.
-
8
Mone H, Mowahid G, Monard S, 2000. The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship. Adv Parasitol 44 :100ā138.
-
9
Jourdane J, Southgate VR, Pages JR, Durand P, Tchuente LAT, 2001. Recent studies on Schistosoma intercalatum: taxonomic status, puzzling distribution, and transmission foci revisited. Mem Inst Oswaldo Cruz 96 :45ā48.
-
10
Barber KE, Mkoji M, Loker ES, 2000. PCR-RFLP analysis of the ITS2 region to identify Schistosoma haematobium and S. bovis from Kenya. Am J Trop Med Hyg 62 :434ā440.
-
11
Ross GC, Southgate VR, Knowles RJ, 1978. Observations on some isozymes of strains of Schistosoma bovis, S. mattheei, S. margrebowiei and S. leiperi.Z Parasitenkd 57 :49ā56.
-
12
Walker TK, Rollinson D, Simpson AJG, 1986. Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. Mol Biochem Parasitol 21 :123ā131.
-
13
Dias Neto E, Pereira de Souza C, Rollinson D, Katz N, Pena SDJ, Sympson AJG, 1993. The random amplification of Polymorphic DNA allows the identification of strains and species of schistosome. Mol Biochem Parasitol 57 :83ā88.
-
14
Hamburger J, He N, Abbasi I, Ramzy RM, Jourdane J, Ruppel A, 2001. Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water. Am J Trop Med Hyg 65 :907ā911.
-
15
Hamburger J, Hoffman O, Kariuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH, 2004. Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71 :765ā773.
-
16
Kariuki HC, Clennon JA, Brady M, Kitron U, Sturrock RF, Ouma JH, Tosha S, Ndzovhu M, Mungai P, Hamburger J, Hoffman O, Pellegrini C, Muchiri EM, King CH, 2004. Distribution patterns and cercarial shedding of Bulinus nasutus and other snails in the Msambweni area, Coast Province, Kenya. Am J Trop Med Hyg 70 :449ā456.
-
17
Sambrook J, Fritsch EF, Maniatis, 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
-
18
Buluwela L, Forster A, Boehm T, Rabbitts TH, 1989. A rapid procedure for colony screening using nylon filters. Nucleic Acids Res 17 :452.
-
19
Rajkovic A, Davis RS, Simonsen N, Rottman F, 1990. A splice leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni.Proc Natl Acad Sci USA 87 :8879ā8883.
-
20
Hamburger J, Turetski T, Kapeller I, Deresievicz R, 1991. Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe. Mol Biochem Parasitol 44 :73ā80.
-
21
Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig P, Hamburger J, 2003. Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated sequence. Am J Trop Med Hyg 69 :324ā330.
-
22
Simpson AJ, Sher A, McCutchan TF, 1982. The genome of Schistosoma mansoni: Isolation of DNA, its size, bases and repetitive sequences. Mol Biochem Parasitol 6 :125ā137.
-
23
Vercruysse J, Southgate VR, Rollinson D, DeClercq D, Sacko M, De Bont J, Mungomba LM, 1994. Studies on transmission and schistosome interactions in Senegal, Mali, and Zambia. Trop Geogr Med 46 :220ā226.
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| Abstract Views | 17 | 17 | 6 |
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DIFFERENTIATION OF SCHISTOSOMA HAEMATOBIUM FROM RELATED SCHISTOSOMES BY PCR AMPLIFYING AN INTER-REPEAT SEQUENCE
IBRAHIM ABBASI
IBRAHIM ABBASI
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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CHARLES H. KING
CHARLES H. KING
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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ROBERT F. STURROCK
ROBERT F. STURROCK
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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CURTIS KARIUKI
CURTIS KARIUKI
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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ERIC MUCHIRI
ERIC MUCHIRI
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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JOSEPH HAMBURGER
JOSEPH HAMBURGER
Kuvin Center, Hebrew University, Hadassah Medical School, Jerusalem, Israel; Center for Global Health and Diseases, Case Western Reserve University, School of Medicine, Cleveland, Ohio; London School of Hygiene and Tropical Medicine, London, United Kingdom; Division of Vector Borne Diseases, Ministry of Health, Nairobi, Kenya; Kenya Medical Research Institute, Nairobi Kenya
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Schistosoma haematobium infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with S. haematobium requires a successful differentiation between S. haematobium and any closely related schistosome species. To enable differential detection of S. haematobium DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified Schistosoma DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of S. haematobium from S. bovis, S. mattheei, S. curassoni, and S. intercalatum (but not from S. margrebowiei) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual S. haematobium transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where S. haematobium is sympatric with the most prevalent other schistosome species.
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- Search for repeated sequences and primers specific for S. haematobium.
- Structure, abundance, and arrangement of the Sh110 repeated sequence.
- Structure, abundance, and arrangement of the Sh110/Sm-Sl PCR product.
- Differential identification of S. haematobium by PCR: detection sensitivity and specificity.
- Detection of S. haematobiumāinfected snails by PCR.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
WHO, 2002. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. World Health Organ Tech Rep Ser 912 :2ā3.
-
2
Loker ES, 1983. A comparative study of the life-histories of mammalian schistosomes. Parasitology 87 :343ā369.
-
3
Rollinson D, Stothard JR, Southgate VR, 2001. Interactions between intermediate snail hosts of the genus Bulinus and schistosomes of the Schistosoma haematobium group. Parasitology 123 (Suppl):S245āS260.
-
4
Chipaux JP, Boulanger D, Bremond P, Campagne G, Vera C, Sellin B, 1997. The WHO Collaborating Centre for Research and Control of schistosomiasis at Niamey, Niger. Mem Inst Oswaldo Cruz 92 :725ā728.
-
5
Woolhouse ME, Chandiwana SK, 1989. Spatial and temporal heterogeneity in the population dynamics of Bulinus globosus and Biomphalaria pfeifferi and in the epidemiology of their infection with schistosomes. Parasitology 98 :21ā34.
-
6
Woolhouse MEJ, Chandiwana SK, 1990. The epidemiology of schistosome infections of snails: taking the theory into the field. Parasitol Today 6 :65ā70.
-
7
Christensen NO, Mutani A, Frandsen F, 1983. A review of the biology and transmission ecology of African bovine species of the genus Schistosoma.Z Parasitenkd 69 :551ā570.
-
8
Mone H, Mowahid G, Monard S, 2000. The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship. Adv Parasitol 44 :100ā138.
-
9
Jourdane J, Southgate VR, Pages JR, Durand P, Tchuente LAT, 2001. Recent studies on Schistosoma intercalatum: taxonomic status, puzzling distribution, and transmission foci revisited. Mem Inst Oswaldo Cruz 96 :45ā48.
-
10
Barber KE, Mkoji M, Loker ES, 2000. PCR-RFLP analysis of the ITS2 region to identify Schistosoma haematobium and S. bovis from Kenya. Am J Trop Med Hyg 62 :434ā440.
-
11
Ross GC, Southgate VR, Knowles RJ, 1978. Observations on some isozymes of strains of Schistosoma bovis, S. mattheei, S. margrebowiei and S. leiperi.Z Parasitenkd 57 :49ā56.
-
12
Walker TK, Rollinson D, Simpson AJG, 1986. Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. Mol Biochem Parasitol 21 :123ā131.
-
13
Dias Neto E, Pereira de Souza C, Rollinson D, Katz N, Pena SDJ, Sympson AJG, 1993. The random amplification of Polymorphic DNA allows the identification of strains and species of schistosome. Mol Biochem Parasitol 57 :83ā88.
-
14
Hamburger J, He N, Abbasi I, Ramzy RM, Jourdane J, Ruppel A, 2001. Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water. Am J Trop Med Hyg 65 :907ā911.
-
15
Hamburger J, Hoffman O, Kariuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH, 2004. Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71 :765ā773.
-
16
Kariuki HC, Clennon JA, Brady M, Kitron U, Sturrock RF, Ouma JH, Tosha S, Ndzovhu M, Mungai P, Hamburger J, Hoffman O, Pellegrini C, Muchiri EM, King CH, 2004. Distribution patterns and cercarial shedding of Bulinus nasutus and other snails in the Msambweni area, Coast Province, Kenya. Am J Trop Med Hyg 70 :449ā456.
-
17
Sambrook J, Fritsch EF, Maniatis, 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
-
18
Buluwela L, Forster A, Boehm T, Rabbitts TH, 1989. A rapid procedure for colony screening using nylon filters. Nucleic Acids Res 17 :452.
-
19
Rajkovic A, Davis RS, Simonsen N, Rottman F, 1990. A splice leader is present on a subset of mRNAs from the human parasite Schistosoma mansoni.Proc Natl Acad Sci USA 87 :8879ā8883.
-
20
Hamburger J, Turetski T, Kapeller I, Deresievicz R, 1991. Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe. Mol Biochem Parasitol 44 :73ā80.
-
21
Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig P, Hamburger J, 2003. Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated sequence. Am J Trop Med Hyg 69 :324ā330.
-
22
Simpson AJ, Sher A, McCutchan TF, 1982. The genome of Schistosoma mansoni: Isolation of DNA, its size, bases and repetitive sequences. Mol Biochem Parasitol 6 :125ā137.
-
23
Vercruysse J, Southgate VR, Rollinson D, DeClercq D, Sacko M, De Bont J, Mungomba LM, 1994. Studies on transmission and schistosome interactions in Senegal, Mali, and Zambia. Trop Geogr Med 46 :220ā226.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 17 | 17 | 6 |
| Full Text Views | 282 | 83 | 1 |
| PDF Downloads | 106 | 33 | 1 |