ANTIBODY RECOGNITION OF HETEROLOGOUS VARIANT SURFACE ANTIGENS AFTER A SINGLE PLASMODIUM FALCIPARUM INFECTION IN PREVIOUSLY NAÏVE ADULTS

SALENNA R. ELLIOTT Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by SALENNA R. ELLIOTT in
Current site
Google Scholar
PubMed
Close
,
PAUL D. PAYNE Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by PAUL D. PAYNE in
Current site
Google Scholar
PubMed
Close
,
MICHAEL F. DUFFY Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by MICHAEL F. DUFFY in
Current site
Google Scholar
PubMed
Close
,
TIMOTHY J. BYRNE Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by TIMOTHY J. BYRNE in
Current site
Google Scholar
PubMed
Close
,
WAI-HONG THAM Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by WAI-HONG THAM in
Current site
Google Scholar
PubMed
Close
,
STEPHEN J. ROGERSON Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by STEPHEN J. ROGERSON in
Current site
Google Scholar
PubMed
Close
,
GRAHAM V. BROWN Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by GRAHAM V. BROWN in
Current site
Google Scholar
PubMed
Close
, and
DAMON P. EISEN Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Victoria, Australia; Queensland Institute of Medical Research, Queensland, Australia; Victorian Infectious Diseases Service, Royal Melbourne Hospital, Victoria, Australia

Search for other papers by DAMON P. EISEN in
Current site
Google Scholar
PubMed
Close
Restricted access

Antibodies to variant surface antigens (VSA) including Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) have been associated with protective antimalarial immunity that appears to be variant-specific. This study of adult returned travelers with a single acute P. falciparum infection investigated whether a primary antibody response includes IgG specific for heterologous VSA. We found that 11 of 14 patients had IgG reactive with up to six P. falciparum lines expressing different heterologous PfEMP1 variants, measured by flow cytometry (reactivity greater than two times the mean of the negative control sera was defined as “positive”), with high reactivity (greater than four times the mean of the negative controls) detected in three patients. The dominant variant(s) recognized differed between seropositive individuals. Despite previous evidence that antibody responses to heterologous VSA are short-lived, seven patients had detectable IgG at > 20 weeks post-infection. Together these data suggest that a single P. falciparum infection can be sufficient to induce antibodies reactive with several PfEMP1 variants, although the repertoire of target epitopes they recognize may still be restricted.

Author Notes

  • 1

    Bull PC, Lowe BS, Kortok M, Molyneux CS, Newbold CI, Marsh K, 1998. Parasite antigens on the infected red cell surface are targets for naturally acquired immunity to malaria. Nat Med 4 :358–360.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2

    Sherman IW, Eda S, Winograd E, 2003. Cytoadherence and sequestration in Plasmodium falciparum: defining the ties that bind. Microbes Infect 5 :897–909.

  • 3

    Marsh K, Howard RJ, 1986. Antigens induced on erythrocytes by P. falciparum: expression of diverse and conserved determinants. Science 231 :150–153.

  • 4

    Giha HA, Staalsoe T, Dodoo D, Elhassan IM, Roper C, Satti GM, Arnot DE, Theander TG, Hviid L, 1999. Nine-year longitudinal study of antibodies to variant antigens on the surface of Plasmodium falciparum-infected erythrocytes. Infect Immun 67 :4092–4098.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5

    Giha HA, Staalsoe T, Dodoo D, Elhassan IM, Roper C, Satti GM, Arnot DE, Hviid L, Theander TG, 1999. Overlapping antigenic repertoires of variant antigens expressed on the surface of erythrocytes infected by Plasmodium falciparum.Parasitology 119 :7–17.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6

    Noviyanti R, Brown GV, Wickham ME, Duffy MF, Cowman AF, Reeder JC, 2001. Multiple var gene transcripts are expressed in Plasmodium falciparum infected erythrocytes selected for adhesion. Mol Biochem Parasitol 114 :227–237.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7

    Elliott SR, Duffy MF, Byrne TJ, Beeson JG, Mann EJ, Wilson DW, Rogerson SJ, Brown GV, 2005. Cross-reactive surface epitopes on chondroitin sulfate A-adherent Plasmodium falciparum-infected erythrocytes are associated with transcription of var2csa.Infect Immun 73 :2848–2856.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8

    Duffy MF, Byrne TJ, Elliott SR, Wilson DW, Rogerson SJ, Beeson JG, Noviyanti R, Brown GV, 2005. Broad analysis reveals a consistent pattern of var gene transcription in Plasmodium falciparum repeatedly selected for a defined adhesion phenotype. Mol Microbiol 56 :774–788.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9

    Beeson JG, Brown GV, Molyneux ME, Mhango C, Dzinjalamala F, Rogerson SJ, 1999. Plasmodium falciparum isolates from infected pregnant women and children are associated with distinct adhesive and antigenic properties. J Infect Dis 180 :464–472.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10

    Kyes S, Pinches R, Newbold CI, 2000. A simple RNA analysis method shows var and rif multigene family expression patterns in Plasmodium falciparum.Mol Biochem Parasitol 105 :311–315.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11

    Taylor HM, Kyes SA, Harris D, Kriek N, Newbold CI, 2000. A study of var gene transcription in vitro using universal var gene primers. Mol Biochem Parasitol 105 :13–23.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12

    Beeson JG, Mann EJ, Elliott SR, Lema VM, Tadesse E, Molyneux ME, Brown GV, Rogerson SJ, 2004. Antibodies to variant surface antigens of Plasmodium falciparum-infected erythrocytes and adhesion inhibitory antibodies are associated with placental malaria and have overlapping and distinct targets. J Infect Dis 189 :540–551.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13

    Fried M, Nosten F, Brockman A, Brabin BJ, Duffy PE, 1998. Maternal antibodies block malaria. Nature 395 :851–852.

  • 14

    Ricke CH, Staalsoe T, Koram K, Akanmori BD, Riley EM, Theander TG, Hviid L, 2000. Plasma antibodies from malaria-exposed pregnant women recognize variant surface antigens on Plasmodium falciparum-infected erythrocytes in a parity-dependent manner and block parasite adhesion to chondroitin sulfate A. J Immunol 165 :3309–3316.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 15

    Peters J, Fowler E, Gatton M, Chen N, Saul A, Cheng Q, 2002. High diversity and rapid changeover of expressed var genes during the acute phase of Plasmodium falciparum infections in human volunteers. Proc Natl Acad Sci USA 99 :10689–10694.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 16

    Lavstsen T, Magistrado P, Hermsen CC, Salanti A, Jensen AT, Sauerwein R, Hviid L, Theander TG, Staalsoe T, 2005. Expression of Plasmodium falciparum erythrocyte membrane protein 1 in experimentally infected humans. Malar J 4 :21.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 17

    Kinyanjui SM, Bull P, Newbold CI, Marsh K, 2003. Kinetics of antibody responses to Plasmodium falciparum-infected erythrocyte variant surface antigens. J Infect Dis 187 :667–674.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18

    Ofori MF, Dodoo D, Staalsoe T, Kurtzhals JA, Koram K, Theander TG, Akanmori BD, Hviid L, 2002. Malaria-induced acquisition of antibodies to Plasmodium falciparum variant surface antigens. Infect Immun 70 :2982–2988.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 19

    Siegrist CA, 2001. Neonatal and early life vaccinology. Vaccine 19 :3331–3346.

Past two years Past Year Past 30 Days
Abstract Views 134 133 16
Full Text Views 271 3 0
PDF Downloads 48 1 0
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save