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Reference Manager
BibTeX
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-
1
Desjeux P, Alvar J, 2003. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitol 97 :3–15.
-
2
Deniau M, Canavate C, Faraut-Gambarelli F, Marty P, 2003. The biological diagnosis of leishmaniasis in HIV-infected patients. Ann Trop Med Parasitol 97 :115–133.
-
3
Mary C, Lamouroux D, Dunan S, Quilici M, 1992. Western blot analysis of antibodies to Leishmania infantum antigens: potential of the 14-kD and 16-kD antigens for diagnosis and epidemiologic purposes. Am J Trop Med Hyg 47 :764–771.
-
4
Carvalho EM, Barral A, Pedral-Sampaio D, Barral-Netto M, Badaro R, Rocha H, Johnson WD Jr, 1992. Immunologic markers of clinical evolution in children recently infected with Leishmania donovani chagasi.J Infect Dis 165 :535–540.
-
5
Berrahal F, Mary C, Roze M, Berenger A, Escoffier K, Lamouroux D, Dunan S, 1996. Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting. Am J Trop Med Hyg 55 :273–277.
-
6
Lachaud L, Chabbert E, Dubessay P, Dereure J, Lamothe J, Dedet JP, Bastien P, 2002. Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers. Parasitology 125 :197–207.
-
7
Mary C, Faraut F, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42 :5249–5255.
-
8
Di Giorgio C, Ridoux O, Delmas F, Azas N, Gasquet M, Timon-David P, 2000. Flow cytometric detection of Leishmania parasites in human monocyte-derived macrophages: application to antileishmanial-drug testing. Antimicrob Agents Chemother 44 :3074–3078.
-
9
Nicolas L, Prina E, Lang T, Milon G, 2002. Real-time PCR for detection and quantitation of Leishmania in mouse tissues. J Clin Microbiol 40 :1666–1669.
-
10
Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP, Bastien P, 2002. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol 40 :210–215.
-
11
Costa CH, Stewart JM, Gomes RB, Garcez LM, Ramos PK, Bozza M, Satoskar A, Dissanayake S, Santos RS, Silva MR, Shaw JJ, David JR, Maguire JH, 2002. Asymptomatic human carriers of Leishmania chagasi.Am J Trop Med Hyg 66 :334–337.
-
12
Bucheton B, Kheir MM, El-Safi SH, Hammad A, Mergani A, Mary C, Abel L, Dessein A, 2002. The interplay between environmental and host factors during an outbreak of visceral leishmaniasis in eastern Sudan. Microbes Infect 4 :1449–1457.
-
13
Le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol 37 :1953–1957.
-
14
Sharma MC, Gupta AK, Das VN, Verma N, Kumar N, Saran R, Kar SK, 2000. Leishmania donovani in blood smears of asymptomatic persons. Acta Trop 76 :195–196.
-
15
Disch J, Oliveira MC, Orsini M, Rabello A, 2004. Rapid clearance of circulating Leishmania kinetoplast DNA after treatment of visceral leishmaniasis. Acta Trop 92 :279–283.
-
16
Martin-Sanchez J, Pineda JA, Morillas-Marquez F, Garcia-Garcia JA, Acedo C, Macias J, 2004. Detection of Leishmania infantum kinetoplast DNA in peripheral blood from asymptomatic individuals at risk for parenterally transmitted infections: relationship between polymerase chain reaction results and other Leishmania infection markers. Am J Trop Med Hyg 70 :545–548.
-
17
Cortes S, Rolao N, Ramada J, Campino L, 2004. PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers. Trans R Soc Trop Med Hyg 98 :12–17.
-
18
Lambson B, Smyth A, Barker DC, 2000. Leishmania donovani: development and characterisation of a kinetoplast DNA probe and its use in the detection of parasites. Exp Parasitol 94 :15–22.
-
19
Bhattacharyya R, Das K, Sen S, Roy S, Majumder HK, 1996. Development of a genus specific primer set for detection of Leishmania parasites by polymerase chain reaction. FEMS Microbiol Lett 135 :195–200.
-
20
Bossolasco S, Gaiera G, Olchini D, Gulletta M, Martello L, Bestetti A, Bossi L, Germagnoli L, Lazzarin A, Uberti-Foppa C, Cinque P, 2003. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J Clin Microbiol 41 :5080–5084.
-
21
Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S, 2005. Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure. J Clin Microbiol 43 :3038–3041.
-
22
Cruz I, Canavate C, Rubio JM, Morales MA, Chicharro C, Laguna F, Jimenez-Mejias M, Sirera G, Videla S, Alvar J, Spanish HIV-Leishmania Study Group, 2002. A nested polymerase chain reaction (Ln-PCR) for diagnosing and monitoring Leishmania infantum infection in patients co-infected with human immunodeficiency virus. Trans R Soc Trop Med. 96 :185–189.
-
23
Pizzuto M, Piazza M, Senese D, Scalamogna C, Calattini S, Corsico L, Persico T, Adriani B, Magni C, Guaraldi G, Gaiera G, Ludovisi A, Gramiccia M, Galli M, Moroni M, Corbellino M, Antinori S, 2001. Role of PCR in diagnosis and prognosis of visceral leishmaniasis in patients coinfected with human immunodeficiency virus type 1. J Clin Microbiol 39 :357–361.
-
24
Marty P, Lelievre A, Quaranta JF, Suffia I, Eulalio M, Gari-Toussaint M, Le Fichoux Y, Kubar J, 1995. Detection by Western blot of four antigens characterizing acute clinical leishmaniasis due to Leishmania infantum.Trans R Soc Trop Med Hyg 89 :690–691.
-
25
Riera C, Fisa R, Lopez P, Ribera E, Carrio J, Falco V, Molina I, Gallego M, Portus M, 2004. Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV-Leishmania coinfection: value in diagnosis and post-treatment follow-up. Eur J Clin Microbiol Infect Dis 23 :899–904.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 1641 | 1489 | 21 |
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REFERENCE VALUES FOR LEISHMANIA INFANTUM PARASITEMIA IN DIFFERENT CLINICAL PRESENTATIONS: QUANTITATIVE POLYMERASE CHAIN REACTION FOR THERAPEUTIC MONITORING AND PATIENT FOLLOW-UP
CHARLES MARY
CHARLES MARY
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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FRANÇOISE FARAUT
FRANÇOISE FARAUT
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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MARIE-PIERRE DROGOUL
MARIE-PIERRE DROGOUL
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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BERNARD XERIDAT
BERNARD XERIDAT
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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NICOLAS SCHLEINITZ
NICOLAS SCHLEINITZ
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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BERNADETTE CUISENIER
BERNADETTE CUISENIER
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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HENRI DUMON
HENRI DUMON
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France; Centre d’Information et de Soins de l’Immunodéficience Humaine, Hopital Sainte Mrguerite, Marseille, France; Polyclinique Clairval, Marseille, France; Medecine Interne, Hopital de la Conception, Marseille, France; Laboratoire de Parasitologie, Hopital du Bocage, Dijon, France
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Quantification of Leishmania infantum DNA in blood samples by an ultrasensitive quantitative polymerase chain reaction (QPCR) detected parasitemias in different clinical presentations. We observed a large range of parasitemias, more than 9 log values, and could determine the threshold between asymptomatic carriage and disease in the Mediterranean area (approximately one parasite/mL of blood. Based on kinetoplast DNA amplification, this assay had a sensitivity of 0.001 parasite DNA equivalents/mL and detected asymptomatic carriage of Leishmania. It detected parasite DNA in 58% of healthy subjects, while an immunoblot detected specific antibodies in only 16%. For initial diagnosis of disease, this quantitative PCR with blood samples constitutes a non-invasive alternative to bone marrow aspiration. Its main applications are monitoring of drug therapy and follow-up of immunodeficient patients for biologic confirmation of relapses.
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-
1
Desjeux P, Alvar J, 2003. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitol 97 :3–15.
-
2
Deniau M, Canavate C, Faraut-Gambarelli F, Marty P, 2003. The biological diagnosis of leishmaniasis in HIV-infected patients. Ann Trop Med Parasitol 97 :115–133.
-
3
Mary C, Lamouroux D, Dunan S, Quilici M, 1992. Western blot analysis of antibodies to Leishmania infantum antigens: potential of the 14-kD and 16-kD antigens for diagnosis and epidemiologic purposes. Am J Trop Med Hyg 47 :764–771.
-
4
Carvalho EM, Barral A, Pedral-Sampaio D, Barral-Netto M, Badaro R, Rocha H, Johnson WD Jr, 1992. Immunologic markers of clinical evolution in children recently infected with Leishmania donovani chagasi.J Infect Dis 165 :535–540.
-
5
Berrahal F, Mary C, Roze M, Berenger A, Escoffier K, Lamouroux D, Dunan S, 1996. Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting. Am J Trop Med Hyg 55 :273–277.
-
6
Lachaud L, Chabbert E, Dubessay P, Dereure J, Lamothe J, Dedet JP, Bastien P, 2002. Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers. Parasitology 125 :197–207.
-
7
Mary C, Faraut F, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42 :5249–5255.
-
8
Di Giorgio C, Ridoux O, Delmas F, Azas N, Gasquet M, Timon-David P, 2000. Flow cytometric detection of Leishmania parasites in human monocyte-derived macrophages: application to antileishmanial-drug testing. Antimicrob Agents Chemother 44 :3074–3078.
-
9
Nicolas L, Prina E, Lang T, Milon G, 2002. Real-time PCR for detection and quantitation of Leishmania in mouse tissues. J Clin Microbiol 40 :1666–1669.
-
10
Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP, Bastien P, 2002. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol 40 :210–215.
-
11
Costa CH, Stewart JM, Gomes RB, Garcez LM, Ramos PK, Bozza M, Satoskar A, Dissanayake S, Santos RS, Silva MR, Shaw JJ, David JR, Maguire JH, 2002. Asymptomatic human carriers of Leishmania chagasi.Am J Trop Med Hyg 66 :334–337.
-
12
Bucheton B, Kheir MM, El-Safi SH, Hammad A, Mergani A, Mary C, Abel L, Dessein A, 2002. The interplay between environmental and host factors during an outbreak of visceral leishmaniasis in eastern Sudan. Microbes Infect 4 :1449–1457.
-
13
Le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol 37 :1953–1957.
-
14
Sharma MC, Gupta AK, Das VN, Verma N, Kumar N, Saran R, Kar SK, 2000. Leishmania donovani in blood smears of asymptomatic persons. Acta Trop 76 :195–196.
-
15
Disch J, Oliveira MC, Orsini M, Rabello A, 2004. Rapid clearance of circulating Leishmania kinetoplast DNA after treatment of visceral leishmaniasis. Acta Trop 92 :279–283.
-
16
Martin-Sanchez J, Pineda JA, Morillas-Marquez F, Garcia-Garcia JA, Acedo C, Macias J, 2004. Detection of Leishmania infantum kinetoplast DNA in peripheral blood from asymptomatic individuals at risk for parenterally transmitted infections: relationship between polymerase chain reaction results and other Leishmania infection markers. Am J Trop Med Hyg 70 :545–548.
-
17
Cortes S, Rolao N, Ramada J, Campino L, 2004. PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers. Trans R Soc Trop Med Hyg 98 :12–17.
-
18
Lambson B, Smyth A, Barker DC, 2000. Leishmania donovani: development and characterisation of a kinetoplast DNA probe and its use in the detection of parasites. Exp Parasitol 94 :15–22.
-
19
Bhattacharyya R, Das K, Sen S, Roy S, Majumder HK, 1996. Development of a genus specific primer set for detection of Leishmania parasites by polymerase chain reaction. FEMS Microbiol Lett 135 :195–200.
-
20
Bossolasco S, Gaiera G, Olchini D, Gulletta M, Martello L, Bestetti A, Bossi L, Germagnoli L, Lazzarin A, Uberti-Foppa C, Cinque P, 2003. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J Clin Microbiol 41 :5080–5084.
-
21
Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S, 2005. Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure. J Clin Microbiol 43 :3038–3041.
-
22
Cruz I, Canavate C, Rubio JM, Morales MA, Chicharro C, Laguna F, Jimenez-Mejias M, Sirera G, Videla S, Alvar J, Spanish HIV-Leishmania Study Group, 2002. A nested polymerase chain reaction (Ln-PCR) for diagnosing and monitoring Leishmania infantum infection in patients co-infected with human immunodeficiency virus. Trans R Soc Trop Med. 96 :185–189.
-
23
Pizzuto M, Piazza M, Senese D, Scalamogna C, Calattini S, Corsico L, Persico T, Adriani B, Magni C, Guaraldi G, Gaiera G, Ludovisi A, Gramiccia M, Galli M, Moroni M, Corbellino M, Antinori S, 2001. Role of PCR in diagnosis and prognosis of visceral leishmaniasis in patients coinfected with human immunodeficiency virus type 1. J Clin Microbiol 39 :357–361.
-
24
Marty P, Lelievre A, Quaranta JF, Suffia I, Eulalio M, Gari-Toussaint M, Le Fichoux Y, Kubar J, 1995. Detection by Western blot of four antigens characterizing acute clinical leishmaniasis due to Leishmania infantum.Trans R Soc Trop Med Hyg 89 :690–691.
-
25
Riera C, Fisa R, Lopez P, Ribera E, Carrio J, Falco V, Molina I, Gallego M, Portus M, 2004. Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV-Leishmania coinfection: value in diagnosis and post-treatment follow-up. Eur J Clin Microbiol Infect Dis 23 :899–904.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1641 | 1489 | 21 |
| Full Text Views | 492 | 11 | 0 |
| PDF Downloads | 135 | 12 | 0 |