• 1

    World Health Organization, 2003. Assessment and Monitoring of Antimalarial Drug Efficacy for the Treatment of Uncomplicated Falciparum Malaria. Geneva: World Health Organization. WHO/HTM/RBM/2003.50.

  • 2

    Basco L, Ringwald P, 2000. Chimiorésistance du paludisme: problèmes de la définition et de l’approche technique. Cah Sante 10 :47–50.

    • Search Google Scholar
    • Export Citation
  • 3

    White NJ, 1998. Preventing antimalarial drug resistance through combinations. Drug Resist Update 1 :3–9.

  • 4

    Trager W, Jensen JB, 1976. Human malaria parasites in continuous culture. Science 193 :673–675.

  • 5

    Mount DL, Nahlen BL, Patchen LC, Churchill FC, 1989. Adaptations of the Saker-Solomons test: simple, reliable colorimetric field assays for chloroquine and its metabolites in urine. Bull World Health Organ 67 :295–300.

    • Search Google Scholar
    • Export Citation
  • 6

    Basco LK, 2004. Molecular epidemiology of malaria in Cameroon. XX. Experimental studies on various factors of in vitro drug sensitivity assays using fresh isolates of Plasmodium falciparum.Am J Trop Med Hyg 70 :474–480.

    • Search Google Scholar
    • Export Citation
  • 7

    Desjardins RE, Canfield CJ, Haynes JD, Chulay JD, 1979. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 16 :710–718.

    • Search Google Scholar
    • Export Citation
  • 8

    Ringwald P, Basco LK, 1999. Comparison of in vivo and in vitro tests of resistance in patients treated with chloroquine in Yaounde, Cameroon. Bull World Health Organ 77 :34–43.

    • Search Google Scholar
    • Export Citation
  • 9

    Basco LK, Ndounga M, Keundjian A, Ringwald P, 2002. Molecular epidemiology of malaria in Cameroon. IX. Characteristics of recrudescent and persistent Plasmodium falciparum infections after chloroquine or amodiaquine treatment in children. Am J Trop Med Hyg 66 :117–123.

    • Search Google Scholar
    • Export Citation
  • 10

    Haynes JD, Diggs CL, Hines FA, Desjardins RE, 1976. Culture of human malaria parasites (P. falciparum). Nature 263 :767–769.

  • 11

    Divo AA, Jensen JB, 1982. Studies on serum requirements for the cultivation of Plasmodium falciparum. II. Medium enrichment. Bull World Health Organ 60 :571–575.

    • Search Google Scholar
    • Export Citation
  • 12

    Tan-ariya P, Brockelman CR, 1983. Continuous cultivation and improved drug responsiveness of Plasmodium falciparum in p-aminobenzoic acid-deficient medium. J Parasitol 69 :353–359.

    • Search Google Scholar
    • Export Citation
  • 13

    Asahi H, Kanazawa T, 1994. Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor. Parasitology 109 :397–401.

    • Search Google Scholar
    • Export Citation
  • 14

    Srivastava K, Puri SK, 2004. Plasmodium falciparum: modified medium composition supports continuous cultivation with foetal bovine serum. Exp Parasitol 108 :74–75.

    • Search Google Scholar
    • Export Citation
  • 15

    Binh VQ, Luty AJF, Kremsner PG, 1997. Differential effects of human serum and cells on the growth of Plasmodium falciparum adapted to serum-free in vitro culture conditions. Am J Trop Med Hyg 57 :594–600.

    • Search Google Scholar
    • Export Citation
  • 16

    Cranmer SL, Magowan C, Liang J, Coppel RL, Cooke BM, 1997. An alternative to serum for cultivation of Plasmodium falciparum in vitro.Trans R Soc Trop Med Hyg 91 :363–365.

    • Search Google Scholar
    • Export Citation
  • 17

    Basco LK, 2003. Molecular epidemiology of malaria in Cameroon. XV. Experimental studies on serum substitutes and supplements and alternative culture media for in vitro drug sensitivity assays using fresh isolates. Am J Trop Med Hyg 69 :168–173.

    • Search Google Scholar
    • Export Citation
  • 18

    Kim HS, Miyake H, Arai M, Wataya Y, 1998. A potent antimalarial activity of 5-fluoroorotate in combination with sulfamonomethoxine against Plasmodium falciparum in vitro and Plasmodium berghei in mice. Parasitol Int 47 :59–67.

    • Search Google Scholar
    • Export Citation
  • 19

    Ringwald P, Meche FS, Bickii J, Basco LK, 1999. In vitro culture and drug sensitivity assay of Plasmodium falciparum with non-serum substitute and acute phase sera. J Clin Microbiol 37 :700–705.

    • Search Google Scholar
    • Export Citation
  • 20

    Basco LK, 2003. Molecular epidemiology of malaria in Cameroon. XVII. Baseline monitoring of atovaquone-resistant Plasmodium falciparum by in vitro drug assays and cytochrome b gene sequence analysis. Am J Trop Med Hyg 69 :179–183.

    • Search Google Scholar
    • Export Citation
  • 21

    Oduola AM, Alexander BM, Weatherly NF, Bowdre JH, Desjardins RE, 1985. Use of non-human plasma for in vitro cultivation and antimalarial drug susceptibility testing of Plasmodium falciparum.Am J Trop Med Hyg 34 :209–215.

    • Search Google Scholar
    • Export Citation
  • 22

    Sax LJ, Rieckmann KH, 1980. Use of rabbit serum in the cultivation of Plasmodium falciparum.J Parasitol 66 :621–624.

  • 23

    Butcher GA, 1979. Factors affecting the in vitro culture of Plasmodium falciparum and Plasmodium knowlesi.Bull World Health Organ 57 (suppl 1):17–26.

    • Search Google Scholar
    • Export Citation
  • 24

    Jensen JB, 1979. Some aspects of serum requirements for continuous cultivation of Plasmodium falciparum.Bull World Health Organ 57 (suppl 1):27–31.

    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 134 64 3
PDF Downloads 23 11 0
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

 

MOLECULAR EPIDEMIOLOGY OF MALARIA IN CAMEROON. XXIII. EXPERIMENTAL STUDIES ON SERUM SUBSTITUTES AND ALTERNATIVE CULTURE MEDIA FOR IN VITRO DRUG SENSITIVITY ASSAYS USING CLINICAL ISOLATES OF PLASMODIUM FALCIPARUM

View More View Less
  • 1 Unité de Recherche Paludologie Afro-Tropicale, Institut de Recherche pour le Développement and Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, Yaounde, Cameroon
Restricted access

Correlation studies on the in vitro drug response of field isolates of Plasmodium falciparum and molecular markers for drug resistance are becoming important as many malaria control programs abandon monotherapies and resort to combination therapies. The standardization and optimization of the in vitro drug sensitivity assay are one of the prerequisites for validating molecular markers in the field. The present study was designed to assess and compare the growth of freshly obtained isolates for at least the first erythrocytic cycle in various culture media and determine the in vitro response to chloroquine in alternative media. Parasite growth was consistently higher in Dulbecco’s modified Eagle’s medium (DME)–human serum, Iscove’s modified Dulbecco’s medium (IMDM)–human serum, RPMI 1640 medium–goat serum, and a serum-free medium containing 1:1 (v/v) mixture of IMDM and F-12 supplemented with an ammonium sulfate fraction of adult bovine serum than in RPMI 1640 medium–human serum mixture. The level of chloroquine response determined in human serum-supplemented DME, IMDM, and RPMI 1640 media did not differ significantly (P > 0.05) from the control (RPMI 1640–human serum). This study suggests that alternative media may be used to optimize parasite growth during the critical initial phase of transition from in vivo to in vitro conditions. The capacity of these media to support long-term cultivation of P. falciparum requires further investigation.

Save