NATURAL ANAPLASMA PHAGOCYTOPHILUM INFECTION OF TICKS AND RODENTS FROM A FOREST AREA OF JILIN PROVINCE, CHINA

WU-CHUN CAO Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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LIN ZHAN Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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JING HE Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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JANET E. FOLEY Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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SAKE J. DE VLAS Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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XIAO-MING WU Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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HONG YANG Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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JAN. H. RICHARDUS Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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J. DIK F. HABBEMA Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California; Department of Public Health, Erasmus MC, University Medical Center Rotterdam, The Netherlands

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Polymerase chain reaction integrated with sequence analysis was carried out to investigate the natural Anaplasma phagocytophilum infection in ticks and rodents from a forest area of Jilin Province, China. Four (4.0%) of 100 Ixodes persulcatus and 2 (0.7%) of 286 Dermacentor silvarum ticks collected by flagging vegetation were positive. Nine (8.8%) of 102 rodents were infected, as well as 2 (2.8%) of 71 I. persulcatus parasitizing on 25 rodents. The nucleotide sequences of 1442-bp A. phagocytophilum 16S rRNA gene amplified from rodents and ticks were identical to each other and to that previously reported in Heilongjiang Province of China (GenBank accession no. AF205140), but different from those of other countries. The sequences of 357-bp partial citrate synthase gene from the above specimens were homologic, and varied from known A. phagocytophilum agents. These findings add new information on the ecologic features of A. phagocytophilum and indicate the threat of anaplasmosis in the area.

Author Notes

Reprint requests: Dr. Wu-Chun Cao, Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, 20 Dong-Da Street, Fengtai District, Beijing 100071, P. R. China, Telephone: (+86) 10-63896082, Fax: (+86) 10-63896082, E-mail: caowc@nic.bmi.ac.cn or caowc2000@yahoo.com.cn.
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