• 1

    Genta RM, 1989. Global prevalence of strongyloidiasis: critical review with epidemiologic insights into the prevention of disseminated disease. Rev Infect Dis 11 :755–767.

    • Search Google Scholar
    • Export Citation
  • 2

    Grove DI, 1996. Human strongyloidiasis. Adv Parasitol 38 :251–309.

  • 3

    Pearson RD, 2002. An Update on the Geohelminths: Ascaris lumbricoides, Hookworms, Trichuris trichiura, and Strongyloides stercoralis. Curr Infect Dis Rep 4 :59–64.

    • Search Google Scholar
    • Export Citation
  • 4

    Lewthwaite P, Gill GV, Hart CA, Beeching NJ, 2005. Gastrointestinal parasites in the immunocompromised. Curr Opin Infect Dis 18 :427–435.

    • Search Google Scholar
    • Export Citation
  • 5

    Horton J, 2000. Albendazole: a review of anthelmintic efficacy and safety in humans. Parasitology 121 (Suppl):S113–S132.

  • 6

    Adenusi AA, Oke AO, Adenusi AO, 2003. Comparison of ivermectin and thiabendazole in the treatment of uncomplicated human Strongyloides stercoralis infection. Afr J Biotechnol 2 :465–469.

    • Search Google Scholar
    • Export Citation
  • 7

    Shikiya K, Zaha O, Niimura S, Uehara T, Ohshiro J, Kinjo F, Saito A, Asato R, 1994. Clinical study on ivermectin against 125 strongyloidiasis patients. Kansenshogaku Zasshi 68 :13–20.

    • Search Google Scholar
    • Export Citation
  • 8

    Marti H, Haji HJ, Savioli L, Chwaya HM, Mgeni AF, Ameir JS, Hatz C, 1996. A comparative trial of a single-dose ivermectin versus three days of albendazole for treatment of Strongyloides stercoralis and other soil-transmitted helminth infections in children. Am J Trop Med Hyg 55 :477–481.

    • Search Google Scholar
    • Export Citation
  • 9

    Ashraf M, Gue CL, Baddour LM, 1996. Case report: Strongyloidiasis refractory to treatment with ivermectin. Am J Med Sci 311 :178–179.

  • 10

    Sangster N, 1996. Pharmacology of anthelmintic resistance. Parasitology 113 :S201–S216.

  • 11

    Taylor MA, Hunt KR, Goodyear KL, 2002. Anthelmintic resistance detection methods. Vet Parasitol 103 :183–194.

  • 12

    Kotze AC, Clifford S, O’Grady J, Behnke JM, McCarthy JS, 2004. An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species. Am J Trop Med Hyg 71 :608–616.

    • Search Google Scholar
    • Export Citation
  • 13

    Jongsuksuntigul P, Intapan PM, Wongsaroj T, Nilpan S, Singthong S, Veerakul S, Maleewong W, 2003. Prevalence of Strongyloides stercoralis infection in northeastern Thailand (agar plate culture detection). J Med Assoc Thai 86 :737–741.

    • Search Google Scholar
    • Export Citation
  • 14

    Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45 :518–521.

    • Search Google Scholar
    • Export Citation
  • 15

    Beaver PC, Jung RC, Cupp EW, 1984. Clinical Parasitology. Ninth edition. Philadelphia: Lea & Febiger.

  • 16

    Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80 :774–792.

    • Search Google Scholar
    • Export Citation
 
 
 

 

 
 
 

 

 

 

 

 

 

A MODIFIED FILTER PAPER CULTURE TECHNIQUE FOR SCREENING OF STRONGYLOIDES STERCORALIS IVERMECTIN SENSITIVITY IN CLINICAL SPECIMENS

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  • 1 Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

An in vitro test for the detection of ivermectin sensitivity of Strongyloides stercoralis in human clinical specimens was developed. The test has a simple procedure that combines parasite culture with a drug assay. It is based on the principle of the drug effect on larval development test, combined with a modified coproculture using the filter paper culture technique. This does not require parasite isolation, and drug sensitivity can be shown in relationship to the dose. This test can be applied in field surveys for anthelmintic sensitivity and for appropriate drug rotation strategies when drug resistance occurs.

Author Notes

Reprint requests: Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan@kku.ac.th.
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