A MODIFIED FILTER PAPER CULTURE TECHNIQUE FOR SCREENING OF STRONGYLOIDES STERCORALIS IVERMECTIN SENSITIVITY IN CLINICAL SPECIMENS

PEWPAN M. INTAPAN Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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THIDARAT K. PRASONGDEE Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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PORNTIP LAUMMAUNWAI Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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KITTISAK SAWANYAWISUTH Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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SAREE SINGTHONG Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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WANCHAI MALEEWONG Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Office of Disease Prevention and Control, Region VI, Department of Disease Control, Ministry of Public Health, Khon Kaen, Thailand

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An in vitro test for the detection of ivermectin sensitivity of Strongyloides stercoralis in human clinical specimens was developed. The test has a simple procedure that combines parasite culture with a drug assay. It is based on the principle of the drug effect on larval development test, combined with a modified coproculture using the filter paper culture technique. This does not require parasite isolation, and drug sensitivity can be shown in relationship to the dose. This test can be applied in field surveys for anthelmintic sensitivity and for appropriate drug rotation strategies when drug resistance occurs.

Author Notes

Reprint requests: Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan@kku.ac.th.
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