ITS-1 DNA SEQUENCE CONFIRMATION OF LEISHMANIA MAJOR AS A CAUSE OF CUTANEOUS LEISHMANIASIS FROM AN OUTBREAK FOCUS IN THE HO DISTRICT, SOUTHEASTERN GHANA

DAVID J. FRYAUFF Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by DAVID J. FRYAUFF in
Current site
Google Scholar
PubMed
Close
,
HANAFI A. HANAFI Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by HANAFI A. HANAFI in
Current site
Google Scholar
PubMed
Close
,
JOHN D. KLENA Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by JOHN D. KLENA in
Current site
Google Scholar
PubMed
Close
,
DAVID F. HOEL Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by DAVID F. HOEL in
Current site
Google Scholar
PubMed
Close
,
MAXWELL APPAWU Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by MAXWELL APPAWU in
Current site
Google Scholar
PubMed
Close
,
WILLIAM ROGERS Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by WILLIAM ROGERS in
Current site
Google Scholar
PubMed
Close
,
NAIKI PUPLAMPU Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by NAIKI PUPLAMPU in
Current site
Google Scholar
PubMed
Close
,
SHIRLEY ODOOM Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by SHIRLEY ODOOM in
Current site
Google Scholar
PubMed
Close
,
MARGARET KWEKU Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by MARGARET KWEKU in
Current site
Google Scholar
PubMed
Close
,
KWADWO KORAM Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by KWADWO KORAM in
Current site
Google Scholar
PubMed
Close
,
MICHAEL D. WILSON Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by MICHAEL D. WILSON in
Current site
Google Scholar
PubMed
Close
,
GREGORY RACZNIAK Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by GREGORY RACZNIAK in
Current site
Google Scholar
PubMed
Close
, and
DANIEL BOAKYE Research Science Directorate; Enteric Disease Research Program; Vector Biology Research Program; Ghana Detachment, U.S. Naval Medical Research Unit 3, Cairo, Egypt; Infectious Disease Directorate, Naval Medical Research Unit, Silver Springs, Maryland, USA; Department of Parasitology; Department of Epidemiology; Department of Entomology; Noguchi Memorial Institute for Medical Research, Accra, Ghana; Ho District Directorate, Ghana Health Service, Ho, Ghana

Search for other papers by DANIEL BOAKYE in
Current site
Google Scholar
PubMed
Close
Restricted access

Author Notes

Reprint requests: David F. Hoel, Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3, PSC 452 Box 141, FPO AE 09835. E-mail: hoeld@namru3.med.navy.mil
  • 1

    WHO, 2000. The Leishmaniases and Leishmania/HIV co-infections. Fact Sheet No. 116.

    • PubMed
    • Export Citation
  • 2

    Appawu M, Owusu-Agyei S, Dadzie S, Asoala V, Anto F, Koram K, Rogers W, Nkrumah F, Hoffman SL, Fryauff DJ, 2003. Malaria transmission dynamics at a site in northern Ghana proposed for testing malaria vaccines. Trop Med Int Health 9 :164–170.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3

    Cao W, Hashibe M, Rao JY, Morgenstern H, Zhang ZF, 2003. Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells. Cancer Detect Prev 27 :397–404.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4

    Mansour NS, Fryauff DJ, Modi GB, Mikhail EM, Youssef FG, 1991. Isolation and characterization of Leishmania major from Phlebotomus papatasi and military personnel in north Sinai, Egypt. Trans R Soc Trop Med Hyg 85 :590–591.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5

    El Tai NO, Osman OF, El Fari M, Presber W, Schönian G, 2000. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg 94 :575–579.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6

    Meredith SE, Zijlstra EE, Schoone GJ, Kroon CC, van Eys GJ, Schaeffer KU, el-Hassan AM, Lawyer PG, 1993. Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material. Arch Inst Pasteur Tunis 70 :419–431.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7

    Schönian G, Nasereddin A, Dinse N, Carola S, Schallig HD, Presber W, Jaffe CL, 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47 :349–358.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8

    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 :4876–4882.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9

    Kumar S, Tamura K, Nei M, 2004. MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5 :150–163.

  • 10

    Sneath PHA, Sokal RR, 1973. Numerical Taxonomy. San Francisco: W.H. Freeman and Company, 230–234.

    • PubMed
    • Export Citation
  • 11

    Jukes TH, Cantor CR, 1969. Evolution of protein molecules. Munro HN, ed. Mammalian Protein Metabolism. New York: Academic Press, 21–132.

    • PubMed
    • Export Citation
  • 12

    Felsenstein J, 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution Int J Org Evolution 39 :783–791.

  • 13

    Abonnenc E, 1972. Les Phlebotomes de la Region Ethiopienne (Diptera, Psychodidae). Paris, ORSTOM.

    • PubMed
    • Export Citation
  • 14

    Niang AA, Geoffroy B, Angel G, Trouillet J, Killick-Kendrick R, Hervey JP, Brunches J, 2000. Les phlebotomes d’Afrique de l’Quest: Institut de recherche pour le development, Institut fondamental d’Afrique noir l’Université Cheikh Anta Diop de Dakar.

    • PubMed
    • Export Citation
  • 15

    Beach R, Young DG, Kiilu G, 1986. New Phlebotomine sand-fly colonies. II. Laboratory colonization of Phlebotomus dubosqui (Diptera, Psychodidae). J Med Entomol 23 :114–115.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 16

    Hoogstraal H, Deitlein DR, Heyneman D, 1962. Leishmaniasis in the Sudan Republic 4. Preliminary observations on man-biting sand flies (Psychodidae: Phlebotomus) in certain Upper Nile endemic areas. Trans R Soc Trop Med Hyg 56 :411–422.

    • PubMed
    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 332 279 14
Full Text Views 353 10 6
PDF Downloads 132 8 4
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save