IS THE HUMAN-INFECTING DIPHYLLOBOTHRIUM PACIFICUM A VALID SPECIES OR JUST A SOUTH AMERICAN POPULATION OF THE HOLARCTIC FISH BROAD TAPEWORM, D. LATUM?

ANDREA ŠKEŘÍKOVÁ Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by ANDREA ŠKEŘÍKOVÁ in
Current site
Google Scholar
PubMed
Close
,
JAN BRABEC Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by JAN BRABEC in
Current site
Google Scholar
PubMed
Close
,
ROMAN KUCHTA Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by ROMAN KUCHTA in
Current site
Google Scholar
PubMed
Close
,
JUAN A. JIMÉNEZ Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by JUAN A. JIMÉNEZ in
Current site
Google Scholar
PubMed
Close
,
HECTOR H. GARCÍA Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by HECTOR H. GARCÍA in
Current site
Google Scholar
PubMed
Close
, and
TOMÁŠ SCHOLZ Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, České Budějovice; Department of Microbiology, Universidad Peruana Cayetano Heredia and Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru

Search for other papers by TOMÁŠ SCHOLZ in
Current site
Google Scholar
PubMed
Close
Restricted access

Using ITS2 gene sequences, the validity of the tapeworm Diphyllobothrium pacificum (Nybelin, 1931), infecting humans on the Pacific coast of South America and in Japan, was assessed. ITS2 sequences of this cestode differed markedly (sequence similarity 79.0–80.2%) from those of the most common human-infecting cestode, the broad fish tapeworm Diphyllobothrium latum (L.), as well as other four species of Diphyllobothrium, including potential human parasites (D. cordatum, D. dendriticum, and D. lanceolatum) and two species of Spirometra (sequence similarity 77.5–81.9%). Interspecific sequence similarity between all but one (D. pacificum) species was 86.1–99.6%, whereas individual isolates of D. dendriticum and D. ditremum exhibited intraspecific sequence similarity of 97.0–98.0% and 98.2–99.9%, respectively. Phylogenetic trees constructed from ITS2 sequences show a markedly distant position of D. pacificum from other species analyzed and also indicate the possible paraphyly of Spirometra.

Author Notes

  • 1

    Muller R, 2002. Worms and Human Disease. Second edition. Wallingford: CABI Publishing.

    • PubMed
    • Export Citation
  • 2

    Chai J-Y, Murrell KD, Lymbery AJ, 2005. Fish-borne parasitic zoonoses: Status and issues. Int J Parasitol 35 :1233–1254.

  • 3

    Williams HH, Jones A, 1994. Parasitic Worms of Fish. London: Taylor & Francis.

    • PubMed
    • Export Citation
  • 4

    Nybelin O, 1931. Säugertier und Vogelcestoden von Juan Fernandez. Skottsberg C, ed. Natural History of Juan Fernandez und Easter Island. Volume 3. 493–523.

    • PubMed
    • Export Citation
  • 5

    Baer JG, 1969. Diphyllobothrium pacificum, a tapeworm from sea lions endemic in man along the coastal area of Peru. J Fish Res Board Can 26 :717–723.

  • 6

    Kamo H, Maejima J, Yazaki S, 1982. Occurrence of human infection with Diphyllobothrium pacificum (Nybelin, 1931) Margolis, 1956 in Japan. Jap J Parasitol 31 :165–170.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7

    Tsuboi T, Torii M, Hirai K, 1993. Light and scanning electron microscopy of Diphyllobothrium pacificum expelled from a man. Jap J Parasitol 42 :422–428.

  • 8

    Yamaguti S, 1951. Studies on the helminth fauna of Japan. Part 47. Cestodes of marine mammals and birds. Jap J Zool 12: 307–314 +2 plates.

    • PubMed
    • Export Citation
  • 9

    Torres P, Franjola R, Figueroa L, Schlatter R, González H, Contreras B, Martín R, 1981. Researches on Pseudophyllidea (Carus, 1813) in the south of Chile. IV Occurrence of Diphyllobothrium dendriticum (Nitszch). J Helminthol 55 :173–187.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10

    Torres P, Franjola R, Pérez J, Auad S, Uherek F, Miranda JC, Flores L, Riquelme J, Salazar S, Hermosilla C, Rojo R, 1989a. Epidemiología de la difilobotriasis en la cuenca del río Valdivia, Chile. Rev Saúde Pública São Paulo 23 :45–57.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11

    Torres P, Torres J, Garrido O, Thibaut J, 1989b. Investigaciones sobre Pseudophyllidea (Carus, 1813) en el sur de Chile. X. Observaciones experimentales sobre la coexistencia de plerocercoides de Diphyllobothrium latum (L.) y Diphyllobothrium dendriticum (Nitszch) en salmónidos de la cuenca del río Valdivia. Arch Med Vet 21 :51–57.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12

    Torres P, Gesche W, Montefusco A, Miranda JC, Dietz P, Huijse R, 1998. Diphyllobothriosis humana y en peces del lago Riñihue, Chile: Efecto de la actividad educativa, distribución estacional y relación con sexo, talla y dieta de los peces. Arch Med Vet 30 :31–45.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13

    Torres P, López JC, Cubillos V, Lobos C, Silva R, 2002. Visceral diphyllobothriosis in a cultured rainbow trout, Oncorhynchus mykiss (Walbaum), in Chile. J Fish Dis 25 :375–379.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14

    Torres P, Cuevas C, Tang M, Barra M, Franjola R, Navarrete N, Montefusco A, Otth L, Wilson G, Puga S, Figueroa L, Cerda O, 2004. Introduced and native fishes as infection foci of Diphyllobothrium spp. in humans and dogs from two localities at Lake Panguipulli in Southern Chile. Comp Parasitol 71 :111–117.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 15

    Santos FLN, de Faro LB, 2005. The first confirmed case of Diphyllobothrium latum in Brazil. Mem Inst Oswaldo Cruz 100 :585–586.

  • 16

    Yamaguti S, 1959. Systema Helminthum, Volume 2. The Cestodes of Vertebrates. New York: Interscience Publishers.

    • PubMed
    • Export Citation
  • 17

    Scholz T, Škeříková A, Hanzelová V, Koubková B, Baruš V, 2003. Resurrection of Proteocephalus sagittus (Grimm, 1872) (Cestoda: Proteocephalidea) based on morphological and molecular data. Syst Parasitol 56 :173–181.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18

    Hall TA, 1999. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 :95–98.

  • 19

    Swofford DL, 1998. PAUP 4.0-Phylogenetic Analysis Using Parsimony. Version 4. Sunderland, MA: Sinauer Associates.

    • PubMed
    • Export Citation
  • 20

    Zhu XQ, Beveridge I, Berger L, Barton D, Gasser RB, 2002. Single-strand conformation polymorphism-based analysis reveals genetic variation within Spirometra erinacei (Cestoda: Pseudophyllidea) from Australia. Mol Cell Probes 16 :159–165.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 21

    Mueller JF, 1937. A repartition of the genus Diphyllobothrium. J Parasitol 23 :308–310.

  • 22

    Schmidt GD, 1986. CRC Handbook of Tapeworm Identification.Boca Raton, FL: CRC Press.

    • PubMed
    • Export Citation
  • 23

    Bray RA, Jones A, Andersen KI, 1994. Order Pseudophyllidea Carus, 1863. Khalil LF, Jones A, Bray RA, eds. Keys to the Cestode Parasites of Vertebrates. Wallingford, UK: CAB International, 205–247.

    • PubMed
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 51 51 0
Full Text Views 313 6 1
PDF Downloads 94 5 1
 
Membership Banner
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save