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-
1
Dedet JP, 2002. Current status of epidemiology of leishmaniases. Farrell JP (ed). Leishmania, World-class Parasites: Volume 4. Boston, MA: Kluwer Academic Publishers, 1–10.
-
2
Castilho TM, Shaw JJ, Floeter-Winter LM, 2003. New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species. J Clin Microbiol 41 :540–546.
-
3
Navin TR, Arana BA, Arana FE, Bermen JD, Cajón JF, 1992. Placebo-controlled clinical trial of sodium stibogluconate (Pentostam) versus ketaconazole for treating cutaneous leishmaniasis in Guatemala. J Infect Dis 165 :528–534.
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4
Marco JD, Barroso PA, Calvopiña M, Kumazawa H, Furuya M, Korenaga M, Cajal SP, Mora MC, Rea MMJ, Borda CE, Basombrío MA, Taranto NJ, Hashiguchi Y, 2005. Species assignation of Leishmania from humans and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in north Argentina. Am J Trop Med Hyg 72 :606–611.
-
5
Shaw JJ, 2002. New World leishmaniasis: the ecology of leishmaniasis and the diversity of leishmanial species in Central and South America. Farrell JP (ed). Leishmania, World-class Parasites: Volume 4. Boston, MA: Kluwer Academic Publishers, 11–31.
-
6
Salomón OD, Sosa Estani S, Rossi GC, Spinelli GR, 2001. Presencia de Lutzomya longipalpis y situación de la leishmaniosis visceral en Argentina. Medicina (B Aires) 61 :174–178.
-
7
Nocito I, Serra E, Montero A, 2002. Visceral involvement due to Leishmania mexicana in a patient with acquired immunodeficiency syndrome. Am J Med 113 :260–262.
-
8
Martín-Sánchez J, Navarro-Mari JM, Pasquau-Liano J, Salomón OD, Morillas-Marquez F, 2004. Visceral leishmaniasis caused by Leishmania infantum in a Spanish patient in Argentina: What is the origin of the infection? Case report. BMC Infect Dis 4 :20.
-
9
Córdoba Lanús E, Pinero JE, González AC, Valladares B, de Grosso ML, Salomón OD, 2005. Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction. Mem Inst Oswaldo Cruz 100 :187–192.
-
10
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: clinical findings and diagnostic approach. Clin Infect Dis 32 :1304–1312.
-
11
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65 :456–465.
-
12
Marfurt J, Nasereddin A, Niederwieser I, Jaffe CL, Beck HP, Felger I, 2003. Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 41 :3147–3153.
-
13
Victoir K, De Doncker S, Cabrera L, Álvarez E, Arévalo J, Llanos-Cuentas A, Le Ray D, Dujardin JC, 2003. Direct identification of Leishmania species in biopsies from patients with American tegumentary leishmaniasis. Trans R Soc Trop Med Hyg 97 :80–87.
-
14
Guizani I, 2004. Molecular tools for studying the epidemiology of leishmaniasis. World Health Organization, eds. Reports of the Scientific Working Group on Leishmaniasis. Geneva: WHO, 54–70.
-
15
Luyo-Acero G, Uezato H, Oshiro M, Takei K, Kariya K, Katakura K, Gómez EAL, Hashiguchi Y, Nonaka S, 2003. Sequence variation of the cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny. Parasitology 128 :483–491.
-
16
Mimori T, Sasaki J, Nakata M, Gomez EAL, Uezato H, Nonaka S, Hashiguchi Y, Furuya M, Saya H, 1998. Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene 210 :179–186.
-
17
World Health Organization, 2004. Report of the Scientific Working Group on Leishmaniasis. Geneva: WHO, 5–6.
-
18
Diosque P, Barnabé C, Padilla AM, Marco JD, Cardozo RM, Cimino RO, Nasser JR, Tibayrenc M, Basombrío MA, 2003. Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas disease in Argentina. Int J Parasitol 33 :997–1003.
-
19
Kato H, Uezato H, Katakura K, Calvopina M, Marco JD, Barroso PA, Gomez EAL, Mimori T, Korenaga M, Iwata H, Nonaka S, Hashiguchi Y, 2005. Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction. Am J Trop Med Hyg 72 :87–93.
-
20
Cupolillo E, Grimaldi G Jr, Momen H, Beverley SM, 1995. Intergenic region typing (IRT): A rapid molecular approach to the characterization and evolution of Leishmania. Mol Biochem Parasitol 73 :145–155.
-
21
Mimori T, Matsumoto T, Calvopiña M, Gomez EA, Saya H, Katakura K, Nonaka S, Shamsuzzaman SM, Hashiguchi Y, 2002. Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World. Acta Trop 81 :197–202.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 216 | 158 | 6 |
| Full Text Views | 403 | 4 | 0 |
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ARE CYTOCHROME B GENE SEQUENCING AND POLYMORPHISM-SPECIFIC POLYMERASE CHAIN REACTION AS RELIABLE AS MULTILOCUS ENZYME ELECTROPHORESIS FOR IDENTIFYING LEISHMANIA SPP. FROM ARGENTINA?
JORGE D. MARCO
JORGE D. MARCO
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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HIROSHI UEZATO
HIROSHI UEZATO
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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TATSUYUKI MIMORI
TATSUYUKI MIMORI
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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PAOLA A. BARROSO
PAOLA A. BARROSO
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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MASATAKA KORENAGA
MASATAKA KORENAGA
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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SHIGEO NONAKA
SHIGEO NONAKA
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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MIGUEL A. BASOMBRÍO
MIGUEL A. BASOMBRÍO
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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NÉSTOR J. TARANTO
NÉSTOR J. TARANTO
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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YOSHIHISA HASHIGUCHI
YOSHIHISA HASHIGUCHI
Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan; Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta /Consejo Nacional de Investigaciones Científicas y Técnicas, Salta, Argentina; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Tumor Genetics & Biology, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan; Instituto de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, San Ramón de la Nueva Orán, Argentina
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Recently, two techniques, polymerase chain reaction (PCR) amplification and sequencing of cytochrome b gene (cyt b gene sequencing) and polymorphism-specific PCR (PS-PCR) were recommended for Leishmania species identification. Before this study, however, the accuracy of these methods had not been tested against the multilocus enzyme electrophoresis, the current gold standard technique on this task. Therefore, a trial was done for the first time to compare the results obtained by these techniques, using 17 Argentinean Leishmania stocks in independent assays. For all the stocks examined, the same results at species level were obtained by the three techniques. Among them, 14 were assigned to L. (Viannia) braziliensis, and three to L. (V.) guyanensis. The two techniques, cyt b gene sequencing and PS-PCR, were able to distinguish between all the proven species responsible for leishmaniases in Argentina. Thus, both techniques were validated and could be used independently for the species designation of Leishmania parasites in the country.
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-
1
Dedet JP, 2002. Current status of epidemiology of leishmaniases. Farrell JP (ed). Leishmania, World-class Parasites: Volume 4. Boston, MA: Kluwer Academic Publishers, 1–10.
-
2
Castilho TM, Shaw JJ, Floeter-Winter LM, 2003. New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species. J Clin Microbiol 41 :540–546.
-
3
Navin TR, Arana BA, Arana FE, Bermen JD, Cajón JF, 1992. Placebo-controlled clinical trial of sodium stibogluconate (Pentostam) versus ketaconazole for treating cutaneous leishmaniasis in Guatemala. J Infect Dis 165 :528–534.
-
4
Marco JD, Barroso PA, Calvopiña M, Kumazawa H, Furuya M, Korenaga M, Cajal SP, Mora MC, Rea MMJ, Borda CE, Basombrío MA, Taranto NJ, Hashiguchi Y, 2005. Species assignation of Leishmania from humans and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in north Argentina. Am J Trop Med Hyg 72 :606–611.
-
5
Shaw JJ, 2002. New World leishmaniasis: the ecology of leishmaniasis and the diversity of leishmanial species in Central and South America. Farrell JP (ed). Leishmania, World-class Parasites: Volume 4. Boston, MA: Kluwer Academic Publishers, 11–31.
-
6
Salomón OD, Sosa Estani S, Rossi GC, Spinelli GR, 2001. Presencia de Lutzomya longipalpis y situación de la leishmaniosis visceral en Argentina. Medicina (B Aires) 61 :174–178.
-
7
Nocito I, Serra E, Montero A, 2002. Visceral involvement due to Leishmania mexicana in a patient with acquired immunodeficiency syndrome. Am J Med 113 :260–262.
-
8
Martín-Sánchez J, Navarro-Mari JM, Pasquau-Liano J, Salomón OD, Morillas-Marquez F, 2004. Visceral leishmaniasis caused by Leishmania infantum in a Spanish patient in Argentina: What is the origin of the infection? Case report. BMC Infect Dis 4 :20.
-
9
Córdoba Lanús E, Pinero JE, González AC, Valladares B, de Grosso ML, Salomón OD, 2005. Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction. Mem Inst Oswaldo Cruz 100 :187–192.
-
10
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: clinical findings and diagnostic approach. Clin Infect Dis 32 :1304–1312.
-
11
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65 :456–465.
-
12
Marfurt J, Nasereddin A, Niederwieser I, Jaffe CL, Beck HP, Felger I, 2003. Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 41 :3147–3153.
-
13
Victoir K, De Doncker S, Cabrera L, Álvarez E, Arévalo J, Llanos-Cuentas A, Le Ray D, Dujardin JC, 2003. Direct identification of Leishmania species in biopsies from patients with American tegumentary leishmaniasis. Trans R Soc Trop Med Hyg 97 :80–87.
-
14
Guizani I, 2004. Molecular tools for studying the epidemiology of leishmaniasis. World Health Organization, eds. Reports of the Scientific Working Group on Leishmaniasis. Geneva: WHO, 54–70.
-
15
Luyo-Acero G, Uezato H, Oshiro M, Takei K, Kariya K, Katakura K, Gómez EAL, Hashiguchi Y, Nonaka S, 2003. Sequence variation of the cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny. Parasitology 128 :483–491.
-
16
Mimori T, Sasaki J, Nakata M, Gomez EAL, Uezato H, Nonaka S, Hashiguchi Y, Furuya M, Saya H, 1998. Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene 210 :179–186.
-
17
World Health Organization, 2004. Report of the Scientific Working Group on Leishmaniasis. Geneva: WHO, 5–6.
-
18
Diosque P, Barnabé C, Padilla AM, Marco JD, Cardozo RM, Cimino RO, Nasser JR, Tibayrenc M, Basombrío MA, 2003. Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas disease in Argentina. Int J Parasitol 33 :997–1003.
-
19
Kato H, Uezato H, Katakura K, Calvopina M, Marco JD, Barroso PA, Gomez EAL, Mimori T, Korenaga M, Iwata H, Nonaka S, Hashiguchi Y, 2005. Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction. Am J Trop Med Hyg 72 :87–93.
-
20
Cupolillo E, Grimaldi G Jr, Momen H, Beverley SM, 1995. Intergenic region typing (IRT): A rapid molecular approach to the characterization and evolution of Leishmania. Mol Biochem Parasitol 73 :145–155.
-
21
Mimori T, Matsumoto T, Calvopiña M, Gomez EA, Saya H, Katakura K, Nonaka S, Shamsuzzaman SM, Hashiguchi Y, 2002. Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World. Acta Trop 81 :197–202.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 216 | 158 | 6 |
| Full Text Views | 403 | 4 | 0 |
| PDF Downloads | 50 | 4 | 0 |