ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Besansky NJ, 1999. Complexities in the analysis of cryptic taxa within the genus Anopheles. Parassitologia 41 :97–100.
-
2
Aspen S, Savage HM, 2003. Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetyl-cholinesterase gene Ace.2. J Am Mosq Control Assoc 19 :323–328.
-
3
Cockburn AF, 1990. A simple and rapid technique for identification of large numbers of individual mosquitoes using DNA hybridization. Arch Insect Biochem Physiol 14 :191–199.
-
4
Nasci RS, Miller BR, 1996. Culicine mosquitoes and the agents they transmit. Beaty BJ, Marquardt WC, eds. The Biology of Diseases Vectors. Niwot: University of Colorado Press, 85–97.
-
5
Turell MJ, Sardelis MR, Dohm DJ, O’Guinn ML, 2001. Potential North American vectors of West Nile virus. Ann N Y Acad Sci 951 :317–324.
-
6
Harbach RE, Harrison BA, Gad AM, 1984. Culex (Culex) molestus Forskal (Diptera: Culicidae): Neotype designation, description, variation, and taxonomic status. Proc Entomol Soc Wash 86 :521–542.
-
7
Mattingly PF, Roseboom LE, Lloyd E, Knight KL, Laven H, Drummond FH, Christophers SR, Shute PG, 1951. The Culex pipiens complex. Trans R Entomol Soc Lond 102 :331–382.
-
8
Byrne K, Nichols RA, 1999. Culex pipiens in London underground tunnels: differentiation between surface and subterranean populations. Heredity 82 :7–15.
-
9
Fonseca DM, Keyghobadi N, Malcolm CA, Schaffner F, Mogi M, Fleischer RC, Wilkerson RC, 2004. Reply to outbreak of West Nile virus in North America. Science 306 :1473–1475.
-
10
Fonseca DM, Keyghobadi N, Malcolm CA, Mehmet C, Schaffner F, Mogi M, Fleischer RC, Wilkerson RC, 2004. Emerging vectors in the Culex pipiens complex. Science 303 :1535–1538.
-
11
Urbanelli S, Silvestrini F, Reisen WK, De Vito E, Bullini L, 1997. Californian hybrid zone between Culex pipiens pipiens and Cx. p. quinquefasciatus revisited (Diptera:Culicidae). J Med Entomol 34 :116–127.
-
12
Samuel PP, Arunachalam N, Hiriyan J, Thenmozhi V, Gajanana A, Satyanarayana K, 2004. Host-feeding pattern of Culex quin-quefasciatus Say and Mansonia annulifera (Theobald) (Diptera: Culicidae), the major vectors of filariasis in a rural area of south India. J Med Entomol 41 :442–446.
-
13
Zinser M, Ramberg F, Willott E, 2004. Culex quinquefasciatus (Diptera: Culicidae) as a potential West Nile virus vector in Tucson, Arizona: Blood meal analysis indicates feeding on both humans and birds. J Insect Sci 4 :20.
-
14
Smith JL, Keyghobadi N, Matrone MA, Escher R, Fonseca DM, 2005. Cross-species comparison of microsatellite loci in the Culex pipiens complex and beyond. Mol Ecol Notes 5 :697–700.
-
15
Smith JL, Fonseca DM, 2004. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Am J Trop Med Hyg 70 :339–345.
-
16
Shaikevich EV, Vinogradova EB, 2004. Differentiation between the urban mosquito Culex pipiens pipiens F. molestus and Culex torrentium (Diptera: Culicidae) by the molecular genetic metehods. Parazitologiia 38 :406–412.
-
17
Vinogradova EB, Shaikevich EV, 2005. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Parazitologiia 39 :574–576.
-
18
Fonseca DM, Atkinson CT, Fleischer RC, 1998. Microsatellite primers for Culex pipiens quinquefasciatus, the vector of avian malaria in Hawaii. Mol Ecol 7 :1617–1619.
-
19
Rozen S, Skaletsky HJ, 2000. Primer3 on the WWW for general users and for biologist programmers. Misener S, Krawetz S, eds. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press, 365–386.
-
20
Excoffier L, Laval G, Schneider S, 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 1 :47–50.
-
21
Pritchard JK, Stephens M, Donnelly P, 2000. Inference of population structure using multilocus genotype data. Genetics 155 :945–959.
-
22
Ellegren H, 2004. Microsatellites: Simple sequences with complex evolution. Nat Rev Genet 5 :435–445.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 530 | 279 | 23 |
| Full Text Views | 614 | 14 | 2 |
| PDF Downloads | 298 | 16 | 4 |
RAPID ASSAY TO IDENTIFY THE TWO GENETIC FORMS OF CULEX (CULEX) PIPIENS L. (DIPTERA: CULICIDAE) AND HYBRID POPULATIONS
CAROLYN M. BAHNCK
CAROLYN M. BAHNCK
Genetics Program, Smithsonian Institution, Washington, DC; Academy of Natural Sciences, Philadelphia, Pennsylvania
Search for other papers by CAROLYN M. BAHNCK in
Current site
Google Scholar
PubMed
Search for other papers by CAROLYN M. BAHNCK in
Current site
Google Scholar
PubMed
DINA M. FONSECA
DINA M. FONSECA
Genetics Program, Smithsonian Institution, Washington, DC; Academy of Natural Sciences, Philadelphia, Pennsylvania
Search for other papers by DINA M. FONSECA in
Current site
Google Scholar
PubMed
Search for other papers by DINA M. FONSECA in
Current site
Google Scholar
PubMed
A previously developed method to identify members of the Culex pipiens complex exploiting polymorphisms in a nuclear intron (acetylcholinesterase [ACE] based-assay) cannot differentiate the two forms of Cx. pipiens: form pipiens and form molestus. Notably, the two forms seem to differ extensively in behavior and physiology and likely have very different epidemiologic importance. Because they are morphologically indistinguishable, molecular methods are critical for the evaluation of their relative importance. Although the two forms of Cx. pipiens have been distinguished using a panel of microsatellite loci, such a protocol is laborious and expensive. We developed a rapid assay based on polymorphisms in the flanking region of a microsatellite locus. Used in conjunction with the ACE-assay, this new assay allows the identification of pure and hybrid populations of the two Cx. pipiens forms as well as those including Cx. quinquefasciatus. We discuss the usefulness of the method as well as limitations to its application.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Besansky NJ, 1999. Complexities in the analysis of cryptic taxa within the genus Anopheles. Parassitologia 41 :97–100.
-
2
Aspen S, Savage HM, 2003. Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetyl-cholinesterase gene Ace.2. J Am Mosq Control Assoc 19 :323–328.
-
3
Cockburn AF, 1990. A simple and rapid technique for identification of large numbers of individual mosquitoes using DNA hybridization. Arch Insect Biochem Physiol 14 :191–199.
-
4
Nasci RS, Miller BR, 1996. Culicine mosquitoes and the agents they transmit. Beaty BJ, Marquardt WC, eds. The Biology of Diseases Vectors. Niwot: University of Colorado Press, 85–97.
-
5
Turell MJ, Sardelis MR, Dohm DJ, O’Guinn ML, 2001. Potential North American vectors of West Nile virus. Ann N Y Acad Sci 951 :317–324.
-
6
Harbach RE, Harrison BA, Gad AM, 1984. Culex (Culex) molestus Forskal (Diptera: Culicidae): Neotype designation, description, variation, and taxonomic status. Proc Entomol Soc Wash 86 :521–542.
-
7
Mattingly PF, Roseboom LE, Lloyd E, Knight KL, Laven H, Drummond FH, Christophers SR, Shute PG, 1951. The Culex pipiens complex. Trans R Entomol Soc Lond 102 :331–382.
-
8
Byrne K, Nichols RA, 1999. Culex pipiens in London underground tunnels: differentiation between surface and subterranean populations. Heredity 82 :7–15.
-
9
Fonseca DM, Keyghobadi N, Malcolm CA, Schaffner F, Mogi M, Fleischer RC, Wilkerson RC, 2004. Reply to outbreak of West Nile virus in North America. Science 306 :1473–1475.
-
10
Fonseca DM, Keyghobadi N, Malcolm CA, Mehmet C, Schaffner F, Mogi M, Fleischer RC, Wilkerson RC, 2004. Emerging vectors in the Culex pipiens complex. Science 303 :1535–1538.
-
11
Urbanelli S, Silvestrini F, Reisen WK, De Vito E, Bullini L, 1997. Californian hybrid zone between Culex pipiens pipiens and Cx. p. quinquefasciatus revisited (Diptera:Culicidae). J Med Entomol 34 :116–127.
-
12
Samuel PP, Arunachalam N, Hiriyan J, Thenmozhi V, Gajanana A, Satyanarayana K, 2004. Host-feeding pattern of Culex quin-quefasciatus Say and Mansonia annulifera (Theobald) (Diptera: Culicidae), the major vectors of filariasis in a rural area of south India. J Med Entomol 41 :442–446.
-
13
Zinser M, Ramberg F, Willott E, 2004. Culex quinquefasciatus (Diptera: Culicidae) as a potential West Nile virus vector in Tucson, Arizona: Blood meal analysis indicates feeding on both humans and birds. J Insect Sci 4 :20.
-
14
Smith JL, Keyghobadi N, Matrone MA, Escher R, Fonseca DM, 2005. Cross-species comparison of microsatellite loci in the Culex pipiens complex and beyond. Mol Ecol Notes 5 :697–700.
-
15
Smith JL, Fonseca DM, 2004. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Am J Trop Med Hyg 70 :339–345.
-
16
Shaikevich EV, Vinogradova EB, 2004. Differentiation between the urban mosquito Culex pipiens pipiens F. molestus and Culex torrentium (Diptera: Culicidae) by the molecular genetic metehods. Parazitologiia 38 :406–412.
-
17
Vinogradova EB, Shaikevich EV, 2005. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae). Parazitologiia 39 :574–576.
-
18
Fonseca DM, Atkinson CT, Fleischer RC, 1998. Microsatellite primers for Culex pipiens quinquefasciatus, the vector of avian malaria in Hawaii. Mol Ecol 7 :1617–1619.
-
19
Rozen S, Skaletsky HJ, 2000. Primer3 on the WWW for general users and for biologist programmers. Misener S, Krawetz S, eds. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press, 365–386.
-
20
Excoffier L, Laval G, Schneider S, 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 1 :47–50.
-
21
Pritchard JK, Stephens M, Donnelly P, 2000. Inference of population structure using multilocus genotype data. Genetics 155 :945–959.
-
22
Ellegren H, 2004. Microsatellites: Simple sequences with complex evolution. Nat Rev Genet 5 :435–445.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 530 | 279 | 23 |
| Full Text Views | 614 | 14 | 2 |
| PDF Downloads | 298 | 16 | 4 |