DNA PROFILING OF HUMAN BLOOD IN ANOPHELINES FROM LOWLAND AND HIGHLAND SITES IN WESTERN KENYA

THOMAS W. SCOTT Department of Entomology, University of California, Davis, California; Kenya Medical Research Institute, Kisumu, Kenya; Department of Entomology, Cornell University, Ithaca, New York; Department of Biological Sciences, State University of New York, Buffalo, New York

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ANDREW K. GITHEKO Department of Entomology, University of California, Davis, California; Kenya Medical Research Institute, Kisumu, Kenya; Department of Entomology, Cornell University, Ithaca, New York; Department of Biological Sciences, State University of New York, Buffalo, New York

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ANDREW FLEISHER Department of Entomology, University of California, Davis, California; Kenya Medical Research Institute, Kisumu, Kenya; Department of Entomology, Cornell University, Ithaca, New York; Department of Biological Sciences, State University of New York, Buffalo, New York

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LAURA C. HARRINGTON Department of Entomology, University of California, Davis, California; Kenya Medical Research Institute, Kisumu, Kenya; Department of Entomology, Cornell University, Ithaca, New York; Department of Biological Sciences, State University of New York, Buffalo, New York

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GUIYUN YAN Department of Entomology, University of California, Davis, California; Kenya Medical Research Institute, Kisumu, Kenya; Department of Entomology, Cornell University, Ithaca, New York; Department of Biological Sciences, State University of New York, Buffalo, New York

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We used polymerase chain reaction (PCR)-based DNA profiling to determine the person from whom Anopheles funestus and An. gambiae collected in natural human habitations obtained their blood meals. Less than 20% of human hosts contributed to > 50% of all blood meals, and 42% were not bitten at all, including people in the age group bitten most often. As expected, bites were unevenly distributed by age (young adults > older adults > children). Use of untreated bed nets by adults, but not children, seemed to redirect bites to children. Multiple blood meals in a single gonotrophic cycle occurred frequently enough to be epidemiologically important (14% for An. funestus and 11% for An. gambiae). Mosquitoes that did not bite a person who slept in the collection house can affect estimation of entomological risk. Mosquito–human interactions did not differ across ecologically and epidemiologically distinct highland and lowland sites.

Author Notes

Reprint requests: Thomas W. Scott, Department of Entomology, University of California, Davis, CA 95616. E-mail: twscott@ucdavis.edu.
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