THE FIRST ISOLATION OF JAPANESE ENCEPHALITIS VIRUS FROM MOSQUITOES COLLECTED FROM MAINLAND AUSTRALIA

ANDREW F. VAN DEN HURK Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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BRIAN L. MONTGOMERY Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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JUDITH A. NORTHILL Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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INA L. SMITH Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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PAUL ZBOROWSKI Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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SCOTT A. RITCHIE Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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JOHN S. MACKENZIE Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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GREG A. SMITH Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Public Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Queensland, Australia; School of Public Health and Tropical Medicine, James Cook University, Cairns, Queensland, Australia; Australian Biosecurity Cooperative Research Centre, Curtin University of Technology, Perth, Western Australia, Australia

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In response to an incursion of Japanese encephalitis virus (JEV) on Cape York Peninsula, Australia, in 2005, 23,144 Culex mosquitoes were processed for virus detection. A single isolate of JEV was obtained from a pool of Culex sitiens subgroup mosquitoes. This is the first reported mosquito isolate of JEV from the Australian mainland.

Author Notes

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    Hanna JN, Ritchie SA, Phillips DA, Shield J, Bailey MC, Mackenzie JS, Poidinger M, McCall BJ, Mills PJ, 1996. An outbreak of Japanese encephalitis in the Torres Strait, Australia, 1995. Med J Aust 165 :256–260.

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    Mackenzie JS, Johansen CA, Ritchie SA, van den Hurk AF, Hall RA, 2002. The emergence and spread of Japanese encephalitis virus in Australasia. Curr Top Microbiol Immunol 267 :49–73.

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    Hanna JN, Ritchie SA, Phillips DA, Lee JM, Hills SL, van den Hurk AF, Pyke AT, Johansen CA, Mackenzie JS, 1999. Japanese encephalitis in north Queensland, Australia, 1998. Med J Aust 170 :533–536.

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    Pyke AT, Smith IL, van den Hurk AF, Northill JA, Chuan TF, Westacott AJ, Smith GA, 2004. Detection of Australasian Flavivirus encephalitic viruses using rapid fluorogenic TaqMan RT-PCR assays. J Virol Methods 117 :161–167.

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    Biggerstaff BJ, 2003. PooledInfRate: A Microsoft Excel Add-In to Compute Prevalence Estimates From Pooled Samples. Fort Collins, CO: Centers for Disease Control and Prevention.

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    Pyke AT, Williams DT, Nisbet DJ, van den Hurk AF, Taylor CT, Johansen CA, Macdonald J, Hall RA, Simmons RJ, Mason RJV, Lee JM, Ritchie SA, Smith GA, Mackenzie JS, 2001. The appearance of a second genotype of Japanese encephalitis virus in the Australasian region. Am J Trop Med Hyg 65 :747–753.

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    Scaramozzino N, Crance JM, Jouan A, DeBriel DA, Stoll F, Garin D, 2001. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol 39 :1922–1927.

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  • 13

    van den Hurk AF, Johansen CA, Zborowski P, Phillips DA, Pyke AT, Mackenzie JS, Ritchie SA, 2001. Flaviviruses isolated from mosquitoes collected during the first outbreak of Japanese encephalitis virus on Cape York Peninsula, Australia. Am J Trop Med Hyg 64 :125–130.

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  • 14

    Johansen CA, Nisbet DJ, Zborowski P, van den Hurk AF, Ritchie SA, Mackenzie JS, 2003. Flavivirus isolations from mosquitoes collected from western Cape York Peninsula, Australia, 1999–2000. J Am Mosq Control Assoc 19 :392–396.

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  • 15

    van den Hurk AF, Nisbet DJ, Foley PN, Ritchie SA, Mackenzie JS, Beebe NW, 2002. Isolation of arboviruses from mosquitoes (Diptera: Culicidae) collected from the Gulf Plains region of northwest Queensland, Australia. J Med Entomol 39 :786– 792.

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  • 16

    Ritchie SA, Rochester W, 2001. Wind-blown mosquitoes and introduction of Japanese encephalitis into Australia. Emerg Infect Dis 7 :900–903.

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    Chapman HF, Hughes JM, Ritchie SA, Kay BH, 2003. Population structure and dispersal of the freshwater mosquitoes Culex annulirostris and Culex palpalis (Diptera: Culicidae) in Papua New Guinea and northern Australia. J Med Entomol 40 :165–169.

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