• 1

    White NJ, 2003. Melioidosis. Lancet 361 :1715–1722.

  • 2

    Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18 :383–416.

  • 3

    Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49 :1075–1078.

    • Search Google Scholar
    • Export Citation
  • 4

    Lowe P, Engler C, Norton R, 2002. Comparison of automated and nonautomated systems for identification of Burkholderia pseudomallei. J Clin Microbiol 40 :4625–4627.

    • Search Google Scholar
    • Export Citation
  • 5

    Petkanjanapong V, Naigowit P, Kondo E, Kanai K, 1992. Use of endotoxin antigens in enzyme-linked immunosorbent assay for the diagnosis of P. pseudomallei infections (melioidosis). Asian Pac J Allergy Immunol 10 :145–150.

    • Search Google Scholar
    • Export Citation
  • 6

    Sirisinha S, Anuntagool N, Dharakul T, Ekpo P, Wongrata-nacheewin S, Naigowit P, Petchclai B, Thamlikitkul V, Suputtamongkol Y, 2000. Recent developments in laboratory diagnosis of melioidosis. Acta Trop 74 :235–245.

    • Search Google Scholar
    • Export Citation
  • 7

    Tomaso H, Pitt TL, Landt O, Al Dahouk S, Scholz HC, Reisinger EC, Sprague LD, Rathmann I, Neubauer H, 2005. Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes. Mol Cell Probes 19 :9–20.

    • Search Google Scholar
    • Export Citation
  • 8

    Wuthiekanun V, Anuntagool N, White NJ, Sirisinha S, 2002. Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis. Am J Trop Med Hyg 66 :759–761.

    • Search Google Scholar
    • Export Citation
  • 9

    Woo PC, Lau SK, Woo GK, Fung AM, Ngan AH, Hui WT, Yuen KY, 2003. Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing. J Clin Microbiol 41 :3973–3977.

    • Search Google Scholar
    • Export Citation
  • 10

    Anuntagool N, Aramsri P, Panichakul T, Wuthiekanun VR, Kinoshita R, White NJ, Sirisinha S, 2000. Antigenic heterogeneity of lipopolysaccharide among Burkholderia pseudomallei clinical isolates. Southeast Asian J Trop Med Public Health 31 (Suppl 1):146–152.

    • Search Google Scholar
    • Export Citation
  • 11

    Anuntagool N, Intachote P, Wuthiekanun V, White NJ, Sirisinha S, 1998. Lipopolysaccharide from nonvirulent Ara+ Burkholderia pseudomallei isolates is immunologically indistinguishable from lipopolysaccharide from virulent Ara-clinical isolates. Clin Diagn Lab Immunol 5 :225–229.

    • Search Google Scholar
    • Export Citation
  • 12

    Pitt TL, Aucken H, Dance DA, 1992. Homogeneity of lipopolysaccharide antigens in Pseudomonas pseudomallei. J Infect 25 :139–146.

  • 13

    Sermswan RW, Wongratanacheewin S, Trakulsomboon S, Thamlikitkul V, 2001. Ribotyping of Burkholderia pseudomallei from clinical and soil isolates in Thailand. Acta Trop 80 :237–244.

    • Search Google Scholar
    • Export Citation
  • 14

    Koonpaew S, Ubol MN, Sirisinha S, White NJ, Chaiyaroj SC, 2000. Genome fingerprinting by pulsed-field gel electrophoresis of isolates of Burkholderia pseudomallei from patients with melioidosis in Thailand. Acta Trop 74 :187–191.

    • Search Google Scholar
    • Export Citation
  • 15

    Taweechaisupapong S, Kaewpa C, Arunyanart C, Kanla P, Homchampa P, Sirisinha S, Proungvitaya T, Wongratanacheewin S, 2005. Virulence of Burkholderia pseudomallei does not correlate with biofilm formation. Microb Pathog 39 :77–85.

    • Search Google Scholar
    • Export Citation
  • 16

    Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M, 2000. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 40 :175–179.

    • Search Google Scholar
    • Export Citation
  • 17

    DeShazer D, Brett PJ, Woods DE, 1998. The type II O-antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence. Mol Microbiol 30 :1081–1100.

    • Search Google Scholar
    • Export Citation
  • 18

    O’Toole G, Kaplan HB, Kolter R, 2000. Biofilm formation as microbial development. Annu Rev Microbiol 54 :49–79.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

LIPOPOLYSACCHARIDE HETEROGENEITY AMONG BURKHOLDERIA PSEUDOMALLEI FROM DIFFERENT GEOGRAPHIC AND CLINICAL ORIGINS

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  • 1 Faculty of Tropical Medicine, and Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; University of Oxford, Oxford, United Kingdom; Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Northern Territory Clinical School, Flinders University, Darwin, Northern Territory, Australia; Melioidosis Research Center, Departments of Biochemistry and Microbiology, Faculty of Medicine, and Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand

Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection.

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