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PULSED-FIELD GEL ELECTROPHORESIS AS A DISCRIMINATORY TYPING TECHNIQUE FOR THE BIOTHREAT AGENT BURKHOLDERIA MALLEI

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  • 1 Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.

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