PULSED-FIELD GEL ELECTROPHORESIS AS A DISCRIMINATORY TYPING TECHNIQUE FOR THE BIOTHREAT AGENT BURKHOLDERIA MALLEI

NARISARA CHANTRATITA Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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MONGKOL VESARATCHAVEST Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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VANAPORN WUTHIEKANUN Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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RACHANEEPORN TIYAWISUTSRI Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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TSEDEV ULZIITOGTOKH Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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ERHAN AKCAY Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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NICHOLAS P. J. DAY Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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SHARON J. PEACOCK Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Biocombinat, State-Owned Biological Enterprise, Ulaanbaatar, Mongolia; Tuberculosis Laboratory, Central Veterinary Control and Research Institute, Ankara, Turkey; Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical; Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

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Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.

Author Notes

  • 1

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  • 2

    Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG, 2003. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 41 :2068–2079.

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  • 3

    Harvey SP, Minter JM, 2005. Ribotyping of Burkholderia mallei isolates. FEMS Immunol Med Microbiol 44 :91–97.

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    Koonpaew S, Ubol MN, Sirisinha S, White NJ, Chaiyaroj SC, 2000. Genome fingerprinting by pulsed-field gel electrophoresis of isolates of Burkholderia pseudomallei from patients with melioidosis in Thailand. Acta Trop 74 :187–191.

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  • 5

    Holden MT, Titball RW, Peacock SJ, Cerdeno-Tarraga AM, Atkins T, Crossman LC, Pitt T, Churcher C, Mungall K, Bentley SD, Sebaihia M, Thomson NR, Bason N, Beacham IR, Brooks K, Brown KA, Brown NF, Challis GL, Cherevach I, Chillingworth T, Cronin A, Crossett B, Davis P, DeShazer D, Feltwell T, Fraser A, Hance Z, Hauser H, Holroyd S, Jagels K, Keith KE, Maddison M, Moule S, Price C, Quail MA, Rabbinowitsch E, Rutherford K, Sanders M, Simmonds M, Songsivilai S, Stevens K, Tumapa S, Vesaratchavest M, Whitehead S, Yeats C, Barrell BG, Oyston PC, Parkhill J, 2004. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A 101 :14240–14245.

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  • 6

    Nierman WC, DeShazer D, Kim HS, Tettelin H, Nelson KE, Feldblyum T, Ulrich RL, Ronning CM, Brinkac LM, Daugherty SC, Davidsen TD, Deboy RT, Dimitrov G, Dodson RJ, Durkin AS, Gwinn ML, Haft DH, Khouri H, Kolonay JF, Madupu R, Mohammoud Y, Nelson WC, Radune D, Romero CM, Sarria S, Selengut J, Shamblin C, Sullivan SA, White O, Yu Y, Zafar N, Zhou L, Fraser CM, 2004. Structural flexibility in the Burkholderia mallei genome. Proc Natl Acad Sci U S A 101 :14246–14251.

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