CORRELATION OF SERUM LEVELS OF MACROPHAGE MIGRATION INHIBITORY FACTOR WITH DISEASE SEVERITY AND CLINICAL OUTCOME IN DENGUE PATIENTS

LIEN-CHENG CHEN Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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HUAN-YAO LEI Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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CHING-CHUAN LIU Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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SHU-CHU SHIESH Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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SHUN-HUA CHEN Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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HSIAO-SHENG LIU Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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YEE-SHIN LIN Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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SHAN-TAIR WANG Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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HUEY-WEN SHYU Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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TRAI-MING YEH Institute of Basic Medical Sciences, Departments of Microbiology and Immunology, Departments of Pediatrics, Departments of Medical Laboratory Sciences and Biotechnology, and Departments of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan

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Dengue virus infection can cause mild dengue fever (DF) or severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Cytokines are believed to be involved in the pathogenesis of dengue infection. However, the role of the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) in dengue infection is unclear. In this study, serum levels of MIF in adult dengue patients with different disease severity and clinical outcome were determined and compared with the levels of other cytokines, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10, and interferon gamma (IFN-γ), in the same patients. Serum levels of MIF, IL-6, and IL-10, but not IFN-γ or TNF-α, were higher in all DHF patients who died than in DHF survivors and DF patients. We conclude that in addition to IL-6 and IL-10, elevated levels of serum MIF are a potential predictor of disease severity and clinical outcome in dengue patients.

Author Notes

Reprint requests: Trai-Ming Yeh, Department of Medical Laboratory Sciences and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China, E-mail: today@mail.ncku.edu.tw.
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