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-
2
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18 :383–416.
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3
Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159 :890–899.
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4
Dance DA, Wuthiekanun V, Naigowit P, White NJ, 1989. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol 42 :645–648.
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5
Lew AE, Desmarchelier PM, 1994. Detection of Pseudomonas pseudomallei by PCR and hybridization. J Clin Microbiol 32 :1326–1332.
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6
Dharakul T, Songsivilai S, Viriyachitra S, Luangwedchakarn V, Tassaneetritap B, Chaowagul W, 1996. Detection of Burkholderia pseudomallei DNA in patients with septicemic melioidosis. J Clin Microbiol 34 :609–614.
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7
Kunakorn M, Markham RB, 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J Clin Microbiol 33 :2131–2135.
-
8
Rattanathongkom A, Sermswan RW, Wongratanacheewin S, 1997. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11 :25–31.
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9
Tomaso H, Pitt TL, Landt O, Dahouk SA, Scholz HC, Reisinger EC, Sprague LD, Rathmann I, Neubauer H, 2005. Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes. Mol Cell Probes 19 :9–20.
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10
Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T, 2000. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74 :247–251.
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11
Haase A, Brennan M, Barrett S, Wood Y, Huffam S, O’Brien D, Currie B, 1998. Evaluation of PCR for diagnosis of melioidosis. J Clin Microbiol 36 :1039–1041.
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12
Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S, 2000. Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63 :146–149.
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13
Winstanley C, Hales BA, Hart CA, 1999. Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. J Med Microbiol 48 :649–656.
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14
Winstanley C, Hart CA, 2000. Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes. J Clin Microbiol 38 :883–885.
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15
Smith-Vaughan HC, Gal D, Lawrie PM, Winstanley C, Sriprakash KS, Currie BJ, 2003. Ubiquity of putative type III secretion genes among clinical and environmental Burkholderia pseudomallei isolates in northern Australia. J Clin Microbiol 41 :883–885.
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16
Walsh AL, Smith MD, Wuthiekanun V, Supputamongkol Y, Chaowagul W, Dance DA, Angus B, White NJ, 1995. Prognostic significance of quantitative bacteremia in septicemic melioidosis. Clin Infect Dis 21 :1498–1500.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 376 | 318 | 16 |
| Full Text Views | 366 | 2 | 0 |
| PDF Downloads | 121 | 2 | 0 |
APPLICATION OF A POLYMERASE CHAIN REACTION TO DETECT BURKHOLDERIA PSEUDOMALLEI IN CLINICAL SPECIMENS FROM PATIENTS WITH SUSPECTED MELIOIDOSIS
DANIEL GAL
DANIEL GAL
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
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MARK MAYO
MARK MAYO
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
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EMMA SPENCER
EMMA SPENCER
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
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ALLEN C. CHENG
ALLEN C. CHENG
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
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BART J. CURRIE
BART J. CURRIE
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Infectious Diseases Unit, Northern Territory Clinical School, Flinders University, Royal Darwin Hospital, Darwin, Northern Territory, Australia
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The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
2
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18 :383–416.
-
3
Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159 :890–899.
-
4
Dance DA, Wuthiekanun V, Naigowit P, White NJ, 1989. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol 42 :645–648.
-
5
Lew AE, Desmarchelier PM, 1994. Detection of Pseudomonas pseudomallei by PCR and hybridization. J Clin Microbiol 32 :1326–1332.
-
6
Dharakul T, Songsivilai S, Viriyachitra S, Luangwedchakarn V, Tassaneetritap B, Chaowagul W, 1996. Detection of Burkholderia pseudomallei DNA in patients with septicemic melioidosis. J Clin Microbiol 34 :609–614.
-
7
Kunakorn M, Markham RB, 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J Clin Microbiol 33 :2131–2135.
-
8
Rattanathongkom A, Sermswan RW, Wongratanacheewin S, 1997. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11 :25–31.
-
9
Tomaso H, Pitt TL, Landt O, Dahouk SA, Scholz HC, Reisinger EC, Sprague LD, Rathmann I, Neubauer H, 2005. Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes. Mol Cell Probes 19 :9–20.
-
10
Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T, 2000. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74 :247–251.
-
11
Haase A, Brennan M, Barrett S, Wood Y, Huffam S, O’Brien D, Currie B, 1998. Evaluation of PCR for diagnosis of melioidosis. J Clin Microbiol 36 :1039–1041.
-
12
Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S, 2000. Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63 :146–149.
-
13
Winstanley C, Hales BA, Hart CA, 1999. Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. J Med Microbiol 48 :649–656.
-
14
Winstanley C, Hart CA, 2000. Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes. J Clin Microbiol 38 :883–885.
-
15
Smith-Vaughan HC, Gal D, Lawrie PM, Winstanley C, Sriprakash KS, Currie BJ, 2003. Ubiquity of putative type III secretion genes among clinical and environmental Burkholderia pseudomallei isolates in northern Australia. J Clin Microbiol 41 :883–885.
-
16
Walsh AL, Smith MD, Wuthiekanun V, Supputamongkol Y, Chaowagul W, Dance DA, Angus B, White NJ, 1995. Prognostic significance of quantitative bacteremia in septicemic melioidosis. Clin Infect Dis 21 :1498–1500.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 376 | 318 | 16 |
| Full Text Views | 366 | 2 | 0 |
| PDF Downloads | 121 | 2 | 0 |