• 1

    Levett PN, 2001. Leptospirosis. Clin Microbiol Rev 14 :296–326.

  • 2

    Shieh WJ, Edwards C, Spiegel R, Zaki SR, 1999. Leptospirosis. Guerrant DL, Walker DH, Weller PF, eds. Tropical Infectious Diseases. Principles, Pathogens & Practice. Philadelphia: Churchill Livingstone, 547–555.

  • 3

    Larson AD, Treick RW, Edwards CL, Cox CD, 1959. Growth studies and plate counting of leptospires. J Bacteriol 77 :361–366.

  • 4

    Faine S AB, Bolin C, Perolat P, 2000. Leptospira and Leptospirosis. Melbourne, Australia: MediSci, 173–174.

  • 5

    Andrew MH, Westwood N, 1971. Use of the Coulter counter to count cells of Streptococcus faecalis.J Appl Bacteriol 34 :441–447.

  • 6

    Smither R, 1975. Use of a Coulter counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli.J Appl Bacteriol 39 :157–165.

    • Search Google Scholar
    • Export Citation
  • 7

    Li RC, Lee SW, Lam JS, 1996. Novel method for assessing post-antibiotic effect by using the Coulter counter. Antimicrob Agents Chemother 40 :1751–1753.

    • Search Google Scholar
    • Export Citation
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

 

ENUMERATING LEPTOSPIRES USING THE COULTER COUNTER

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  • 1 Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Author Notes

Reprint requests: MAJ Clinton K. Murray, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388, E-mail: Clinton.Murray@amedd.army.mil.
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