ENUMERATING LEPTOSPIRES USING THE COULTER COUNTER

CHRISTINA M. HUMBERD Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

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CLINTON K. MURRAY Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

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SAUNDRA K. STUART Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

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BARBARA A. REEB Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

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DUANE R. HOSPENTHAL Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

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DOI:
https://doi.org/10.4269/ajtmh.2005.73.962
Volume/Issue:
Volume 73: Issue 5
Page(s):
962–963
Restricted access
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The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Author Notes

Reprint requests: MAJ Clinton K. Murray, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388, E-mail: Clinton.Murray@amedd.army.mil.
Copyright:
Copyright 2005 The American Society of Tropical Medicine 2005
Received:
26 Apr 2005
|
Accepted:
27 May 2005
|
Published Online:
Nov 2005
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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