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  • 1 Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas; the Department of Medicine and Department of Clinical Investigation, Brooke Army Medical Center, Fort Sam Houston, Texas

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Author Notes

Reprint requests: MAJ Clinton K. Murray, MC, USA, Infectious Disease (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234, Telephone: (210) 916-4355, Fax: (210) 916-0388, E-mail: Clinton.Murray@amedd.army.mil.