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-
1
World Health Organization, 2002. Control of Neurocysticercosis: Report by the Secretariat. Fifty-fifth World Health Assembly; Provisional Agenda item 13.18. Washington, DC: World Health Organization. Report no. A55/23.
-
2
Garcia HH, Gilman RH, Tsang VC, Gonzalez AE, 1997. Clinical significance of neurocysticercosis in endemic villages. The Cysticercosis Working Group in Peru. Trans R Soc Trop Med Hyg 91 :176–178.
-
3
Medina MT, Rosas E, Rubio-Donnadieu F, Sotelo J, 1990. Neurocysticercosis as the main cause of late-onset epilepsy in Mexico. Arch Intern Med 150 :325–327.
-
4
Palacio LG, Jimenez I, Garcia HH, Jimenez ME, Sanchez JL, Noh J, Ahn L, Mora O, Giraldo M, Tsang VC, 1998. Neurocysticercosis in persons with epilepsy in Medellin, Colombia. The Neuroepidemiological Research Group of Antioquia. Epilepsia 39 :1334–1339.
-
5
Rajbhandari KC, 2004. Epilepsy in Nepal. Can J Neurol Sci 31 :257–260.
-
6
White AC Jr, 1997. Neurocysticercosis: a major cause of neurological disease worldwide. Clin Infect Dis 24 :101–113.
-
7
Brand JA, Tsang VC, 1989. A rapid immunoblot assay (Western blot) to detect specific antibodies for human immunodeficiency virus, Schistosoma mansoni, and Taenia solium (cysticercosis). J Immunoassay 10 :237–255.
-
8
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
9
Tsang VC, Pilcher JA, Zhou W, Boyer AE, Kamango-Sollo EI, Rhoads ML, Murrell KD, Schantz PM, Gilman RH, 1991. Efficacy of the immunoblot assay for cysticercosis in pigs and modulated expression of distinct IgM/IgG activities to Taenia solium antigens in experimental infections. Vet Immunol Immunopathol 29 :69–78.
-
10
Pan American Health Organization, 1997. PAHO/WHO Informal Consultation on the Taeniosis/Cysticercosis Complex. Brasília, Brazil, August 23–25, 1995. Washington, DC: Pan American Health Organization, 6–9.
-
11
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99 :257–261.
-
12
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86 :1001–1007.
-
13
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Noh J, Tsang VC, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41 :2577–2586.
-
14
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133 :115–124.
-
15
del Brutto OH, Rajshekhar V, White AC Jr, Tsang VC, Nash TE, Takayanagui OM, Schantz PM, Evans CA, Flisser A, Correa D, Botero D, Allan JC, Sarti E, Gonzalez AE, Gilman RH, Garcia HH, 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology 57 :177–183.
-
16
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, Tsang VC, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164 :1007–1009.
-
17
Hancock K, Tsang VC, 1986. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods 92 :167–176.
-
18
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 :248–254.
-
19
Tsang VC, Greene RM, Pilcher JB, 1995. Optimization of the covalent conjugating procedure (NaIO4) of horseradish peroxidase to antibodies for use in enzyme-linked immunosorbent assay. J Immunoassay 16 :395–418.
-
20
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 :32–35.
-
21
Hernandez M, Beltran C, Garcia E, Fragoso G, Gevorkian G, Fleury A, Parkhouse M, Harrison L, Sotelo J, Sciutto E, 2000. Cysticercosis: towards the design of a diagnostic kit based on synthetic peptides. Immunol Lett 71 :13–17.
-
22
Fleury A, Beltran C, Ferrer E, Garate T, Harrison LJ, Parkhouse RM, Garcia E, Fragoso G, Costa-Cruz J, Biondi G, Agapejev S, Sciutto E, 2003. Application of synthetic peptides to the diagnosis of neurocysticercosis. Trop Med Int Health 8 :1124–1130.
-
23
Butler JE, 2004. Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays. Methods Mol Med 94 :333–372.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 727 | 699 | 310 |
| Full Text Views | 309 | 2 | 0 |
| PDF Downloads | 62 | 2 | 0 |
SERODIAGNOSIS OF NEUROCYSTICERCOSIS USING SYNTHETIC 8-KD PROTEINS: COMPARISON OF ASSAY FORMATS
CHRISTINA M. SCHEEL
CHRISTINA M. SCHEEL
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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AZRA KHAN
AZRA KHAN
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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KATHY HANCOCK
KATHY HANCOCK
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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HECTOR H. GARCIA
HECTOR H. GARCIA
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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ARMANDO E. GONZALEZ
ARMANDO E. GONZALEZ
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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ROBERT H. GILMAN
ROBERT H. GILMAN
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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VICTOR C. W. TSANG
VICTOR C. W. TSANG
Department of Cellular Biology, University of Georgia, Athens, Georgia; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurológicas, Lima, Peru; Departments of Microbiology and Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School Public Health, Baltimore, Maryland
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The assay of choice for serological detection of cysticercosis in humans and pigs is the enzyme-linked immunoelectrotransfer blot (EITB), a Western blot assay that relies on the use of seven lentil-lectin–purified glycoproteins (LLGPs) derived from Taenia solium metacestodes. The EITB is has a sensitivity of 98% and a specificity of 100% in detecting cysticercosis, yet scarcity of native source material and the labor-intensive process of metacestode purification hinder its practicality. These limitations have necessitated the reproduction of the EITB antigens in synthetic forms. Four chemically synthesized LLGP antigens, TS14, TS18var1, TSRS1, and TSRS2var1, were assayed individually by enzyme-linked immunosorbent assay (ELISA) and Western blot for immunoreactivity against a large cohort of sera from clinically defined neurocysticercosis patients. The sensitivity and specificity of all four of these antigens using the ELISA format were well below the standards set by the LLGP EITB, whereas results of the Western blot format closely mirrored those of the LLGP EITB.
Author Notes
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-
1
World Health Organization, 2002. Control of Neurocysticercosis: Report by the Secretariat. Fifty-fifth World Health Assembly; Provisional Agenda item 13.18. Washington, DC: World Health Organization. Report no. A55/23.
-
2
Garcia HH, Gilman RH, Tsang VC, Gonzalez AE, 1997. Clinical significance of neurocysticercosis in endemic villages. The Cysticercosis Working Group in Peru. Trans R Soc Trop Med Hyg 91 :176–178.
-
3
Medina MT, Rosas E, Rubio-Donnadieu F, Sotelo J, 1990. Neurocysticercosis as the main cause of late-onset epilepsy in Mexico. Arch Intern Med 150 :325–327.
-
4
Palacio LG, Jimenez I, Garcia HH, Jimenez ME, Sanchez JL, Noh J, Ahn L, Mora O, Giraldo M, Tsang VC, 1998. Neurocysticercosis in persons with epilepsy in Medellin, Colombia. The Neuroepidemiological Research Group of Antioquia. Epilepsia 39 :1334–1339.
-
5
Rajbhandari KC, 2004. Epilepsy in Nepal. Can J Neurol Sci 31 :257–260.
-
6
White AC Jr, 1997. Neurocysticercosis: a major cause of neurological disease worldwide. Clin Infect Dis 24 :101–113.
-
7
Brand JA, Tsang VC, 1989. A rapid immunoblot assay (Western blot) to detect specific antibodies for human immunodeficiency virus, Schistosoma mansoni, and Taenia solium (cysticercosis). J Immunoassay 10 :237–255.
-
8
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
9
Tsang VC, Pilcher JA, Zhou W, Boyer AE, Kamango-Sollo EI, Rhoads ML, Murrell KD, Schantz PM, Gilman RH, 1991. Efficacy of the immunoblot assay for cysticercosis in pigs and modulated expression of distinct IgM/IgG activities to Taenia solium antigens in experimental infections. Vet Immunol Immunopathol 29 :69–78.
-
10
Pan American Health Organization, 1997. PAHO/WHO Informal Consultation on the Taeniosis/Cysticercosis Complex. Brasília, Brazil, August 23–25, 1995. Washington, DC: Pan American Health Organization, 6–9.
-
11
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99 :257–261.
-
12
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86 :1001–1007.
-
13
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Noh J, Tsang VC, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41 :2577–2586.
-
14
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133 :115–124.
-
15
del Brutto OH, Rajshekhar V, White AC Jr, Tsang VC, Nash TE, Takayanagui OM, Schantz PM, Evans CA, Flisser A, Correa D, Botero D, Allan JC, Sarti E, Gonzalez AE, Gilman RH, Garcia HH, 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology 57 :177–183.
-
16
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, Tsang VC, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164 :1007–1009.
-
17
Hancock K, Tsang VC, 1986. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods 92 :167–176.
-
18
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 :248–254.
-
19
Tsang VC, Greene RM, Pilcher JB, 1995. Optimization of the covalent conjugating procedure (NaIO4) of horseradish peroxidase to antibodies for use in enzyme-linked immunosorbent assay. J Immunoassay 16 :395–418.
-
20
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 :32–35.
-
21
Hernandez M, Beltran C, Garcia E, Fragoso G, Gevorkian G, Fleury A, Parkhouse M, Harrison L, Sotelo J, Sciutto E, 2000. Cysticercosis: towards the design of a diagnostic kit based on synthetic peptides. Immunol Lett 71 :13–17.
-
22
Fleury A, Beltran C, Ferrer E, Garate T, Harrison LJ, Parkhouse RM, Garcia E, Fragoso G, Costa-Cruz J, Biondi G, Agapejev S, Sciutto E, 2003. Application of synthetic peptides to the diagnosis of neurocysticercosis. Trop Med Int Health 8 :1124–1130.
-
23
Butler JE, 2004. Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays. Methods Mol Med 94 :333–372.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 727 | 699 | 310 |
| Full Text Views | 309 | 2 | 0 |
| PDF Downloads | 62 | 2 | 0 |