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-
1
White NJ, 1997. Assessment of the pharmacodynamic properties of antimalarial drugs in vivo. Antimicrob Agents Chemother 41 :1413–1422.
-
2
MacPherson GG, Warrell MJ, White NJ, Looareesuwan S, Warrell DA, 1985. Human cerebral malaria. A quantitative ultra-structural analysis of parasitized erythrocyte sequestration. Am J Pathol 119 :385–401.
-
3
Turner GDH, Morrison H, Jones M, Davis TME, Looareesuwan S, Buley ID, Gatter KC, Newbold CI, Pukrittayakamee S, 1994. An immunohistochemical study of the pathology of fatal malaria. Am J Pathol 145 :1057–1069.
-
4
Nguyen PH, Day N, Pram TD, Ferguson DJ, White NJ, 1995. Intraleucocytic malaria pigment and prognosis in severe malaria. Trans R Soc Trop Med Hyg 89 :200–204.
-
5
Metzger WG, Mordmuller BG, Kremsner PG, 1995. Malaria pigment in leucocytes. Trans R Soc Trop Med Hyg 89 :637–638.
-
6
Amodu OK, Adeyemo AA, Olumese PE, Gbadegesin RA, 1998. Intraleucocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg 92 :54–96.
-
7
Lyke KE, Diallo DA, Dicko A, Kone A, Coulibaly D, Guindo A, Cissoko Y, Sangare L, Coulibaly S, Dakouo B, Taylor TE, Doumbo OK, Plowe CV, 2003. Association of intraleukocytic Plasmodium falciparum malaria pigment with disease severity, clinical manifestations, and prognosis in severe malaria. Am J Trop Med Hyg 69 :253–259.
-
8
Luty AJ, Perkins DJ, Lell B, Schmidt-Ott R, Lehman LG, Luckner D, Greve B, Matousek P, Herbich K, Schmid D, Weinberg JB, Kremsner PG, 2000. Low interleukin-12 activity in severe Plasmodium falciparum malaria. Infect Immun 68 :3909–3915.
-
9
Wildling E, Winkler S, Kremsner PG, Brandts C, Jenne L, Wernsdorfer WH, 1995. Malaria epidemiology in the province of Moyen Ogooué, Gabon. Trop Med Parasitol 46 :77–82.
-
10
Sylla EH, Kun JF, Kremsner PG, 2000. Mosquito distribution and entomological inoculation rates in three malaria-endemic areas in Gabon. Trans R Soc Trop Med Hyg 94 :652–656.
-
11
Planche T, Krishna S, Kombila M, Engel K, Faucher JF, Ngou-Milama E, Kremsner PG, 2001. Comparison of methods for the rapid laboratory assessment of children with malaria. Am J Trop Med Hyg 65 :599–602.
-
12
DeLong ER, DeLong DM, Clarke-Pearson DL, 1988. Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 44 :837–845.
-
13
Day NP, Pham TD, Phan TL, Dinh XS, Pham PL, Ly VC, Tran TH, Nguyen TH, Bethell DB, Nguyan HP, Tran TH, White NJ, 1996. Clearance kinetics of parasites and pigment-containing leukocytes in severe malaria. Blood 88 :4694–4700.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 343 | 326 | 134 |
| Full Text Views | 199 | 2 | 0 |
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ASSESSMENT OF A SIMPLIFIED METHOD FOR COUNTING LEUKOCYTIC MALARIA PIGMENT
BERTRAND LELL
BERTRAND LELL
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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MICHEL A. MISSINOU
MICHEL A. MISSINOU
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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SAADOU ISSIFOU
SAADOU ISSIFOU
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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PIERRE-BLAISE MATSIEGUI
PIERRE-BLAISE MATSIEGUI
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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CHRISTOPHER H. O. OLOLA
CHRISTOPHER H. O. OLOLA
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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TERRIE E. TAYLOR
TERRIE E. TAYLOR
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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PETER G. KREMSNER
PETER G. KREMSNER
Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute, Kilifi, Kenya; College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan
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Severe and fatal malaria is associated with the increased presence of malaria hemozoin in peripheral phagocytes. Large studies of this relationship are hampered by the fact that identifying and counting phagocytes on thick blood smears is time consuming. Distinguishing which mononuclear cells are monocytes and which granulocytes are neutrophils requires time and careful training. In this study, we evaluated a simplified method in which only the proportions of hemozoin-containing mononuclear cells and granulocytes are counted. Thick blood films from 471 Gabonese children with malaria were evaluated. We found a linear relationship and a strong correlation between the proportions of hemozoin-containing monocytes versus mononuclear cells (r = 0.85) and neutrophils versus polymorphonuclear cells (r = 0.93), respectively. The two methods had similar predictive values, as estimated by receiver operating characteristics curves. This simplified method can be used to estimate the amount of extra-erythrocytic pigment in peripheral blood, and we suggest that it may be particularly suitable for very large studies.
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-
1
White NJ, 1997. Assessment of the pharmacodynamic properties of antimalarial drugs in vivo. Antimicrob Agents Chemother 41 :1413–1422.
-
2
MacPherson GG, Warrell MJ, White NJ, Looareesuwan S, Warrell DA, 1985. Human cerebral malaria. A quantitative ultra-structural analysis of parasitized erythrocyte sequestration. Am J Pathol 119 :385–401.
-
3
Turner GDH, Morrison H, Jones M, Davis TME, Looareesuwan S, Buley ID, Gatter KC, Newbold CI, Pukrittayakamee S, 1994. An immunohistochemical study of the pathology of fatal malaria. Am J Pathol 145 :1057–1069.
-
4
Nguyen PH, Day N, Pram TD, Ferguson DJ, White NJ, 1995. Intraleucocytic malaria pigment and prognosis in severe malaria. Trans R Soc Trop Med Hyg 89 :200–204.
-
5
Metzger WG, Mordmuller BG, Kremsner PG, 1995. Malaria pigment in leucocytes. Trans R Soc Trop Med Hyg 89 :637–638.
-
6
Amodu OK, Adeyemo AA, Olumese PE, Gbadegesin RA, 1998. Intraleucocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg 92 :54–96.
-
7
Lyke KE, Diallo DA, Dicko A, Kone A, Coulibaly D, Guindo A, Cissoko Y, Sangare L, Coulibaly S, Dakouo B, Taylor TE, Doumbo OK, Plowe CV, 2003. Association of intraleukocytic Plasmodium falciparum malaria pigment with disease severity, clinical manifestations, and prognosis in severe malaria. Am J Trop Med Hyg 69 :253–259.
-
8
Luty AJ, Perkins DJ, Lell B, Schmidt-Ott R, Lehman LG, Luckner D, Greve B, Matousek P, Herbich K, Schmid D, Weinberg JB, Kremsner PG, 2000. Low interleukin-12 activity in severe Plasmodium falciparum malaria. Infect Immun 68 :3909–3915.
-
9
Wildling E, Winkler S, Kremsner PG, Brandts C, Jenne L, Wernsdorfer WH, 1995. Malaria epidemiology in the province of Moyen Ogooué, Gabon. Trop Med Parasitol 46 :77–82.
-
10
Sylla EH, Kun JF, Kremsner PG, 2000. Mosquito distribution and entomological inoculation rates in three malaria-endemic areas in Gabon. Trans R Soc Trop Med Hyg 94 :652–656.
-
11
Planche T, Krishna S, Kombila M, Engel K, Faucher JF, Ngou-Milama E, Kremsner PG, 2001. Comparison of methods for the rapid laboratory assessment of children with malaria. Am J Trop Med Hyg 65 :599–602.
-
12
DeLong ER, DeLong DM, Clarke-Pearson DL, 1988. Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 44 :837–845.
-
13
Day NP, Pham TD, Phan TL, Dinh XS, Pham PL, Ly VC, Tran TH, Nguyen TH, Bethell DB, Nguyan HP, Tran TH, White NJ, 1996. Clearance kinetics of parasites and pigment-containing leukocytes in severe malaria. Blood 88 :4694–4700.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 343 | 326 | 134 |
| Full Text Views | 199 | 2 | 0 |
| PDF Downloads | 39 | 2 | 0 |