ISOLATION OF ESCHERICHIA COLI O157:H7 FROM FECAL SAMPLES OF COWS IN VIETNAM

NOBORU NAKASONE Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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HOANG HUY TRAN Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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MINH BINE NGUYEN Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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NAOMI HIGA Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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CLAUDIA TOMA Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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TIANYAN SONG Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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YOSHIO ICHINOSE Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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MASAAKI IWANAGA Division of Bacterial Pathogenesis, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Seihi Public Health Center, Nagasaki, Japan

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Enterohemorrhagic Escherichia coli O157:H7 was isolated for the first time in Vietnam. Shiga toxin–producing E. coli were isolated from 8 of 100 cows examined. The two strains showing serotype O157:H7 carried the eae, ehxA, and stx2c genes, but the other six were negative for the eae gene.

Author Notes

  • 1

    Toma C, Lu Y, Higa N, Nakasone N, Chinen I, Baschkier A, Rivas M, Iwanaga M, 2003. Multiplex PCR assay for identification of human diarrheagenic Escherichia coli. J Clin Microbiol 41 :2669–2671.

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    Machado J, Grimont F, Grimont PAD, 2000. Identification of Escherichia coli flagella types by restriction of the amplified fliC gene. Res Microbiol 151 :535–546.

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    Toma C, Martinez Espinosa E, Song T, Miliwebsky E, Chinen I, Iyoda S, Iwanaga M, Rivas M, 2004. Distribution of putative adhesins in different seropathotypes of Shiga toxin-producing Escherichia coli. J Clin Microbiol 42 :4937–4946.

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    Sandner L, Eguiarte LE, Navarro A, Cravioto A, Souza V, 2001. The elements of the locus of enterocyte effacement in human and wild mammal isolates of Escherichia coli: evolution by assemblage or disruption? Microbiology 147 :3149–3158.

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  • 5

    Weiner M, Dacko J, Osek J, 2004. Molecular analysis of enterotoxigenic, Shiga toxigenic and enteroaggregative Escherichia coli strains isolated from suckling piglets with diarrhoea by the use of pulsed-field electrophoresis. Bull Vet Inst Pulawy 48 :225–231.

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    Kullas H, Coles M, Rhyan J, Clark L, 2002. Prevalence of Escherichia coli serogroups and human virulence factors in faeces of urban Canada geese (Branta canadensis). Int J Environ Health Res 12 :153–162.

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  • 7

    Nguyen BM, Phung DC, Nakasone N, Toma C, Higa N, Iyoda S, Iwanaga M, 2004. Shiga-toxin producing Escherichia coli in Vietnam. Trop Med Health 32 :339–341.

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