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1
OPS/OMS, 2003. XIIa Reunión de la Comisión Intergubernamental del Cono Sur para la eliminación de Triatoma infestans y la interrupción de la transmisión transfusional de la Tripanosomiasis Americana (INCOSUR/Chagas). Santiago, Chile: World Health Organization.
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2
Lorca M, Garcia A, Contreras M, Schenone H, Rojas A, 2001. Evaluation of a Triatoma infestans elimination program by the decrease of Trypanosoma cruzi infection frequency in children younger than 10 years, Chile, 1991–1998. Am J Trop Med Hyg 65 :861–864.
-
3
Lent H, Wygodzinsky P, 1979. Revision of the triatominae (Reduviidae Hemiptera), and their significance as vector of Chagas’s disease. Bull Am Mus Nat Hist 163 :123–520.
-
4
Canals M, Cruzat L, Molina MC, Ferreira A, Cattan PE, 2001. Blood host sources of Mepraia spinolai (Heteroptera: Reduviidae), wild vector of Chagas disease in Chile. J Med Entomol 38 :303–307.
-
5
Canals M, Ehrenfeld M, Cattan PE, 2000. Situación de Mepraia spinolai vector silvestre de la enfermedad de Chagas en Chile, en relación con otros vectores desde la perspectiva de sus fuentes de alimentación. Rev Med Chile 128: 1108–1112.
-
6
Barreto M, 1964. Reservatórios do Trypanosoma cruzi nas Américas. Rev Brasil Malariol D Trop 16 :27.
-
7
Telford S, Tonn R, 1982. Dynamics of Trypanosoma cruzi in populations of a primary reservoir, Didelphis marsupialis in the highland of Venezuela. Bol Ofic Sanit Panam 93 :341.
-
8
Schenone H, Contreras MC, Borgono JM, Maturana R, Salinas P, Sandoval L, Rojas A, Tello P, Villarroel F, 1991. Panorama general de la epidemiología de la enfermedad de Chagas en Chile. Bol Chil Parasitol 46 :19–30.
-
9
Ulloa M, Traslaviña M, Alcaino H, 1989. Enfermedad de Chagas en caninos y caprinos sinantrópicos de la Provincia del Choapa, IV Región, Chile. Parasitol al día 13 :120–124.
-
10
Whiting C, 1946. Contribución al estudio de las reservas de parásitos de la enfermedad de Chagas en Chile. Primeros hallazgos en Chile de mamíferos silvestres infestados por Trypanosoma cruzi. Rev Chil Hig Med Prev 8 :69–102.
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11
Duran J, Videla M, Apt W, 1989. Enfermedad de Chagas en una comunidad de pequeños mamíferos simpátricos de la Reserva Nacional de Las Chinchillas. Parasitol al Día 13 :15–20.
-
12
Jimenez J, Lorca M, 1990. Trypanosomiasis americana en vertebrados silvestres y su relación con el vector Triatoma spinolai. Arch Med Vet XXII :179–183.
-
13
WHO, 1991. Control of Chagas Disease. WHO Technical Report Series 811. Geneva: World Health Organization, 97.
-
14
Wincker P, Britto C, Borges J, Cardoso M, Oelemann W, Morel C, 1994. Use a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51 :771–777.
-
15
Solari A, Campillay R, Ortiz S, Wallace A, 2001. Identification of Trypanosoma cruzi genotypes circulating in Chilean Chagasic patients. Exp Parasitol 97 :226–233.
-
16
Dias-Lima A, Sherlock I, 2000. Sylvatic vectors invading houses and the risk of emergence of cases of Chagas disease in Salvador, State of Bahia, Northeast Brazil. Mem Inst Oswaldo Cruz 95 :611–613.
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TRYPANOSOMA CRUZI INFECTION IN WILD MAMMALS FROM A CHAGASIC AREA OF CHILE
MARLENE ROZAS
MARLENE ROZAS
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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CAREZZA BOTTO-MAHAN
CAREZZA BOTTO-MAHAN
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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XIMENA CORONADO
XIMENA CORONADO
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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SYLVIA ORTIZ
SYLVIA ORTIZ
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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PEDRO E. CATTAN
PEDRO E. CATTAN
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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ALDO SOLARI
ALDO SOLARI
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile; Department of Ecological Sciences, Faculty of Sciences, University of Chile, Santiago, Chile; Department of Biological Sciences, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile
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We report results of PCR-DNA based detection of Trypanosoma cruzi in wild mammal reservoirs from a chagasic area of Chile. We analyzed 157 blood samples from wild mammals including the marsupial Thylamis elegans and the rodents Octodon degus, Phyllotis darwini, and Abrothrix olivaceus. In addition, 42 blood samples from goats (i.e., a peridomestic mammal) were analyzed. Blood samples were used to extract DNA, and PCR was performed using the amplification of minicircle DNA sequences. Southern analysis was used to confirm diagnosis with a universal probe of P32-labeled kinetoplast DNA. Altogether, 51% of the wild and 36% of the peridomestic mammals were infected with T. cruzi. These findings suggest that the real T. cruzi infection levels in wild and peridomiciliary reservoirs are higher than those previously determined with serological and parasitological conventional methods. The relevance of our results is discussed in term of the risk factor of infection in human.
Author Notes
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Reference Manager
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-
1
OPS/OMS, 2003. XIIa Reunión de la Comisión Intergubernamental del Cono Sur para la eliminación de Triatoma infestans y la interrupción de la transmisión transfusional de la Tripanosomiasis Americana (INCOSUR/Chagas). Santiago, Chile: World Health Organization.
-
2
Lorca M, Garcia A, Contreras M, Schenone H, Rojas A, 2001. Evaluation of a Triatoma infestans elimination program by the decrease of Trypanosoma cruzi infection frequency in children younger than 10 years, Chile, 1991–1998. Am J Trop Med Hyg 65 :861–864.
-
3
Lent H, Wygodzinsky P, 1979. Revision of the triatominae (Reduviidae Hemiptera), and their significance as vector of Chagas’s disease. Bull Am Mus Nat Hist 163 :123–520.
-
4
Canals M, Cruzat L, Molina MC, Ferreira A, Cattan PE, 2001. Blood host sources of Mepraia spinolai (Heteroptera: Reduviidae), wild vector of Chagas disease in Chile. J Med Entomol 38 :303–307.
-
5
Canals M, Ehrenfeld M, Cattan PE, 2000. Situación de Mepraia spinolai vector silvestre de la enfermedad de Chagas en Chile, en relación con otros vectores desde la perspectiva de sus fuentes de alimentación. Rev Med Chile 128: 1108–1112.
-
6
Barreto M, 1964. Reservatórios do Trypanosoma cruzi nas Américas. Rev Brasil Malariol D Trop 16 :27.
-
7
Telford S, Tonn R, 1982. Dynamics of Trypanosoma cruzi in populations of a primary reservoir, Didelphis marsupialis in the highland of Venezuela. Bol Ofic Sanit Panam 93 :341.
-
8
Schenone H, Contreras MC, Borgono JM, Maturana R, Salinas P, Sandoval L, Rojas A, Tello P, Villarroel F, 1991. Panorama general de la epidemiología de la enfermedad de Chagas en Chile. Bol Chil Parasitol 46 :19–30.
-
9
Ulloa M, Traslaviña M, Alcaino H, 1989. Enfermedad de Chagas en caninos y caprinos sinantrópicos de la Provincia del Choapa, IV Región, Chile. Parasitol al día 13 :120–124.
-
10
Whiting C, 1946. Contribución al estudio de las reservas de parásitos de la enfermedad de Chagas en Chile. Primeros hallazgos en Chile de mamíferos silvestres infestados por Trypanosoma cruzi. Rev Chil Hig Med Prev 8 :69–102.
-
11
Duran J, Videla M, Apt W, 1989. Enfermedad de Chagas en una comunidad de pequeños mamíferos simpátricos de la Reserva Nacional de Las Chinchillas. Parasitol al Día 13 :15–20.
-
12
Jimenez J, Lorca M, 1990. Trypanosomiasis americana en vertebrados silvestres y su relación con el vector Triatoma spinolai. Arch Med Vet XXII :179–183.
-
13
WHO, 1991. Control of Chagas Disease. WHO Technical Report Series 811. Geneva: World Health Organization, 97.
-
14
Wincker P, Britto C, Borges J, Cardoso M, Oelemann W, Morel C, 1994. Use a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51 :771–777.
-
15
Solari A, Campillay R, Ortiz S, Wallace A, 2001. Identification of Trypanosoma cruzi genotypes circulating in Chilean Chagasic patients. Exp Parasitol 97 :226–233.
-
16
Dias-Lima A, Sherlock I, 2000. Sylvatic vectors invading houses and the risk of emergence of cases of Chagas disease in Salvador, State of Bahia, Northeast Brazil. Mem Inst Oswaldo Cruz 95 :611–613.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 657 | 597 | 188 |
| Full Text Views | 238 | 10 | 2 |
| PDF Downloads | 60 | 10 | 2 |