Authors:
, , , , , , , , , , , , , , , , , and
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-
1
Dias JC, Schofield CJ, 1999. The evolution of Chagas disease (American trypanosomiasis). Control after 90 years since Carlos Chagas discovery. Mem Inst Oswaldo Cruz 94 :103–121.
-
2
Dodd RY, 1998. Transmission of parasites by blood transfusion. Vox Sang 74 :161–163.
-
3
Schumunis GA, 1999. Prevention of transfusional Trypanosoma cruzi infection in Latin America. Mem Inst Oswaldo Cruz 94 :93–101.
-
4
Carvalho MF, de Franco MF, Sooares VA, 1997. Amastigotes forms of Trypanosoma cruzi detected in a renal allograft. Rev Inst Med Trop Sao Paulo 39 :223–226.
-
5
Castro CN, Alves MT, Macedo VO, 1983. Importancia da repeticao do xenodiagnistico para avaliacao da parasitemia na fase cronica da doenca de Chagas. Rev Soc Bras Med Trop 22 :19–23.
-
6
Luz ZM, Coutinho MG, Cancado JR, Krettli AU, 1994. Hemoculture sensitive technique in the detection of Trypanosoma cruzi in chagasic patients in the chronic phase of Chagas disease. Rev Soc Bras Med Trop 27 :143–148.
-
7
Schechter M, Flint JE, Voller A, Guhi E, Marinkelle CJ, Miles MA, 1983. Purified T. cruzi specific glycoprotein for discriminative serological diagnosis of South American trypanosomiasis. Lancet 2 :939–941.
-
8
Ronaldo MC, D’Escoffier LN, Salles JM, Goldenberg S, 1991. Characterization and expression of proteases during T. cruzi metaciclogenesis. Exp Parasitol 73 :44–51.
-
9
Krautz GM, Peterson JD, Godsel LM, Krettli AU, Engman DM, 1998. Human antibody responses of Trypanosoma cruzi 70-kD heat-shock proteins. Am J Trop Med Hyg 58 :137–143.
-
10
Ahmed H, Schott EJ, Gauthier JD, Vasta GR, 2003. Superoxide dismutase from the oyster parasite Perkinsus marinus: purification, biochemical characterization, and development of a plate microassay for activity. Anal Biochem 318 :132–141.
-
11
Kabiri M, Steverding D, 2001. Identification of a developmentally regulated iron superoxide dismutase of Trypanosome brucei. J Biochem 360 :173–177.
-
12
Quesada JM, Entrala E, Fernández-Ramos C, Marín C, Sánchez-Moreno M, 2001. Phytomonas spp.: superoxide dismutase in plant trypanosomes. Mol Biochem Parasitol 115 :123–127.
-
13
Pérez-Fuentes R, Guégan JF, Barnabé C, López-Colombo A, Salgado-Rosas H, Torres-Rasgado E, Briones B, Romero-Diaz M, Ramos-Jiménez J, Sánchez-Guillén MC, 2003. Severity of chronic Chagas disease is associated with cytokine/antioxidant imbalance in chronically infected individuals. Int J Parasitol 33 :293–299.
-
14
Marín C, Hitos AB, Rodríguez-Gonzalez I, Dollet M, Sánchez-Moreno M, 2004. Phytomonas iron superoxide dismutase: a possible molecular marker. FEMS Microbiol Lett 234 :69–74.
-
15
Ismail SA, Paranchuk W, Skeiky YA, Reed SG, Bhatia A, Gedamu L, 1997. Molecular cloning and characterization of two iron superoxide dismutase cDNAs from Trypanosoma cruzi.Mol Biochem Parasitol 86 :187–197.
-
16
Marín C, Rodriguez-Gonzalez I, Hitos AB, Rosales MJ, Dollet M, Sánchez-Moreno M, 2004. Purification and characterization of two iron superoxide dismutase of Phytomonas sp. isolated from Euphorbia characias (plant trypanosomatids). Parasitology 129 :79–86.
-
17
Paoletti F, Mocali A, 1990. Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186 :209–265.
-
18
Bécuwe P, Gratepanche S, Fourmaux MN, van Beeumen J, Samyn B, Mercereau-Puijalon O, Touzel JP, Slomianny C, Camus D, Dive D, 1996. Characterization of iron-dependent endogenous superoxide dismutase of Plasmodium falciparum.Mol Biochem Parasitol 76 :125–134.
-
19
Beyer WF, Fridovich I, 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161 :559–566.
-
20
Camargo ME, 1966. Fluorescent antibody test for serodiagnosis technical modification employing preserved cultured forms of T. cruzi in slide test. Rev Inst Med Trop Sao Paulo 8 :227–234.
-
21
Camargo ME, Hshino S, Sequeira GRV, 1973. Hemagglutination, with preserved, sensitized cells, a practical test for routine serological diagnosis of American trypanosomiasis. Rev Inst Med Trop Sao Paulo 15 :81–85.
-
22
Uttaro AD, Mirkin N, Rider MH, Michels PAM, Opperdoes FR, 1999. Phytomonas sp. as a model of trypanosomatid metabolism and drug. Mem Inst Oswaldo Cruz 94 (Suppl II):C19.
-
23
Bannister JV, Bannister WH, Rottilio G, 1987. Aspects of the structure, function and application of superoxide dismutases. Crit Rev Biochem 22 :111–180.
-
24
Velasco-Castrejón O, Valdespino JL, Tapia CR, Salvatierra B, Guzmán-Bracho C, Magos C, 1992. Seroepidemiología de la enfermedad de Chagas en México. Salud Publica Mex 34 :186–196.
-
25
Guzmán-Bracho C, García L, Verdugo JF, Guerrero S, Torres M, Ramírez C, Veslcao-Castrejón O, 1998. Riesgo de transmisión de Tripanosoma cruzi por transfusión de sangre en México. Rev Panam Salud Publica 4 :94–99.
-
26
UNDP/World Bank/WHO, 1997. Special Programme for Research and Training in Tropical Diseases. TDR News 52 :2.
-
27
Zavala-Velázquez JE, 2003. La enfermdad de Chagas en el Estado de Yucatán,México. Rev Biomed 14 :35–43.
-
28
Camargo ME, Segura EL, Kagan IG, Pacheco-Souza JM, Carvalheiro JR, Yanovsky JF, 1987. Normalización del diagnóstico sexológico de la enfermedad de Chagas en Las Américas: evaluación de tres años de colaboración. Bol Oficina Sanit Panam 102 :449–463.
-
29
Sousa-Jurado F, Zumaquero JL, Reyes PA, Cruz-García A, Guzmán-Bracho C, Monteón VM, 2004. Factores bióticos y abióticos que determinan la seroprevalencia de anticuerpos contra Trypanosoma cruzi en el municipio de Bravo, Puebla, México. Salud Publica Mex 46 :39–48.
-
30
Secretaria de Salud, PROY-NOM-032-SSA2, 2000. Para la Vigilancia, Prevención y Control de Enfermedades Transmitidas por Vector. Mexico City, Mexico: Diario Oficial de la Federación, November 21, 2000. Second sección.
-
31
Ramos-Echeverría AA, Monteon-Padilla VM, Reyes PA, 1993. Detección de anticuerpos contra Tripanosoma cruzi en donadores de sangre. Salud Publica Mex 35 :1–9.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1365 | 1295 | 317 |
| Full Text Views | 298 | 11 | 2 |
| PDF Downloads | 68 | 13 | 2 |
USE OF AN IRON SUPEROXIDE DISMUTASE EXCRETED BY TRYPANOSOMA CRUZI IN THE DIAGNOSIS OF CHAGAS DISEASE: SEROPREVALENCE IN RURAL ZONES OF THE STATE OF QUERETARO, MEXICO
MARÍA E. VILLAGRÁN
MARÍA E. VILLAGRÁN
Laboratorio de Tripanosomiasis Americana, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico; Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
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CLOTILDE MARÍN
CLOTILDE MARÍN
Laboratorio de Tripanosomiasis Americana, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico; Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
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ISABEL RODRÍGUEZ-GONZALEZ
ISABEL RODRÍGUEZ-GONZALEZ
Laboratorio de Tripanosomiasis Americana, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico; Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
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JOSE A. DE DIEGO
JOSE A. DE DIEGO
Laboratorio de Tripanosomiasis Americana, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico; Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
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MANUEL SÁNCHEZ-MORENO
MANUEL SÁNCHEZ-MORENO
Laboratorio de Tripanosomiasis Americana, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico; Departamento de Parasitología, Facultad de Ciencias, Instituto de Biotecnología, Universidad de Granada, Granada, Spain; Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
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Four iron superoxide dismutase (SODI, SODII, SODIII, and SODIV) activities with pI values of 6.9, 6.8, 5.25, and 3.8, respectively, were isolated from epimastigote forms of the Maracay strain of Trypanosoma cruzi cultivated at 28°C in Grace’s medium supplemented with 10% heat-inactivated fetal bovine serum. The activity of SODe (pI 3.8), which coincides with that of SODIV, was detected in Grace’s medium without serum in which T. cruzi epimastigotes were cultivated for 24 hours at 28°C. SODe, which was excreted into the medium by the parasite, was immunogenic and antibodies to SODe were detected in serum to a dilution of 1:2,500 by Western blot. The role of SODe is related to the establishment of the parasite within the host, and its high immunogenicity and specificity make it a useful molecular marker in diagnosing infection with this parasite. To validate a Western blot result using SODe as a antigen fraction, 1,029 sera of individuals from 11 municipalities in the state of Queretaro, Mexico were analyzed. Sampling was done randomly and results were compared with those for the same sera with three conventional serologic methods: an enzyme-linked immunosorbent assay (ELISA), indirect hemagglutination (IHA), and an indirect immunofluorescence assay (IFA) to detect antibodies to T. cruzi SODe. Samples that were positive by these three techniques were also positive by the Western blot method. The seroprevalence values for SODe (8.16% by ELISA and Western blot) in Queretaro were considerably higher than those reported in regions of Mexico considered to be endemic for Chagas disease. These results support the use of SODe in the serodiagnosis of Chagas disease.
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-
1
Dias JC, Schofield CJ, 1999. The evolution of Chagas disease (American trypanosomiasis). Control after 90 years since Carlos Chagas discovery. Mem Inst Oswaldo Cruz 94 :103–121.
-
2
Dodd RY, 1998. Transmission of parasites by blood transfusion. Vox Sang 74 :161–163.
-
3
Schumunis GA, 1999. Prevention of transfusional Trypanosoma cruzi infection in Latin America. Mem Inst Oswaldo Cruz 94 :93–101.
-
4
Carvalho MF, de Franco MF, Sooares VA, 1997. Amastigotes forms of Trypanosoma cruzi detected in a renal allograft. Rev Inst Med Trop Sao Paulo 39 :223–226.
-
5
Castro CN, Alves MT, Macedo VO, 1983. Importancia da repeticao do xenodiagnistico para avaliacao da parasitemia na fase cronica da doenca de Chagas. Rev Soc Bras Med Trop 22 :19–23.
-
6
Luz ZM, Coutinho MG, Cancado JR, Krettli AU, 1994. Hemoculture sensitive technique in the detection of Trypanosoma cruzi in chagasic patients in the chronic phase of Chagas disease. Rev Soc Bras Med Trop 27 :143–148.
-
7
Schechter M, Flint JE, Voller A, Guhi E, Marinkelle CJ, Miles MA, 1983. Purified T. cruzi specific glycoprotein for discriminative serological diagnosis of South American trypanosomiasis. Lancet 2 :939–941.
-
8
Ronaldo MC, D’Escoffier LN, Salles JM, Goldenberg S, 1991. Characterization and expression of proteases during T. cruzi metaciclogenesis. Exp Parasitol 73 :44–51.
-
9
Krautz GM, Peterson JD, Godsel LM, Krettli AU, Engman DM, 1998. Human antibody responses of Trypanosoma cruzi 70-kD heat-shock proteins. Am J Trop Med Hyg 58 :137–143.
-
10
Ahmed H, Schott EJ, Gauthier JD, Vasta GR, 2003. Superoxide dismutase from the oyster parasite Perkinsus marinus: purification, biochemical characterization, and development of a plate microassay for activity. Anal Biochem 318 :132–141.
-
11
Kabiri M, Steverding D, 2001. Identification of a developmentally regulated iron superoxide dismutase of Trypanosome brucei. J Biochem 360 :173–177.
-
12
Quesada JM, Entrala E, Fernández-Ramos C, Marín C, Sánchez-Moreno M, 2001. Phytomonas spp.: superoxide dismutase in plant trypanosomes. Mol Biochem Parasitol 115 :123–127.
-
13
Pérez-Fuentes R, Guégan JF, Barnabé C, López-Colombo A, Salgado-Rosas H, Torres-Rasgado E, Briones B, Romero-Diaz M, Ramos-Jiménez J, Sánchez-Guillén MC, 2003. Severity of chronic Chagas disease is associated with cytokine/antioxidant imbalance in chronically infected individuals. Int J Parasitol 33 :293–299.
-
14
Marín C, Hitos AB, Rodríguez-Gonzalez I, Dollet M, Sánchez-Moreno M, 2004. Phytomonas iron superoxide dismutase: a possible molecular marker. FEMS Microbiol Lett 234 :69–74.
-
15
Ismail SA, Paranchuk W, Skeiky YA, Reed SG, Bhatia A, Gedamu L, 1997. Molecular cloning and characterization of two iron superoxide dismutase cDNAs from Trypanosoma cruzi.Mol Biochem Parasitol 86 :187–197.
-
16
Marín C, Rodriguez-Gonzalez I, Hitos AB, Rosales MJ, Dollet M, Sánchez-Moreno M, 2004. Purification and characterization of two iron superoxide dismutase of Phytomonas sp. isolated from Euphorbia characias (plant trypanosomatids). Parasitology 129 :79–86.
-
17
Paoletti F, Mocali A, 1990. Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186 :209–265.
-
18
Bécuwe P, Gratepanche S, Fourmaux MN, van Beeumen J, Samyn B, Mercereau-Puijalon O, Touzel JP, Slomianny C, Camus D, Dive D, 1996. Characterization of iron-dependent endogenous superoxide dismutase of Plasmodium falciparum.Mol Biochem Parasitol 76 :125–134.
-
19
Beyer WF, Fridovich I, 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161 :559–566.
-
20
Camargo ME, 1966. Fluorescent antibody test for serodiagnosis technical modification employing preserved cultured forms of T. cruzi in slide test. Rev Inst Med Trop Sao Paulo 8 :227–234.
-
21
Camargo ME, Hshino S, Sequeira GRV, 1973. Hemagglutination, with preserved, sensitized cells, a practical test for routine serological diagnosis of American trypanosomiasis. Rev Inst Med Trop Sao Paulo 15 :81–85.
-
22
Uttaro AD, Mirkin N, Rider MH, Michels PAM, Opperdoes FR, 1999. Phytomonas sp. as a model of trypanosomatid metabolism and drug. Mem Inst Oswaldo Cruz 94 (Suppl II):C19.
-
23
Bannister JV, Bannister WH, Rottilio G, 1987. Aspects of the structure, function and application of superoxide dismutases. Crit Rev Biochem 22 :111–180.
-
24
Velasco-Castrejón O, Valdespino JL, Tapia CR, Salvatierra B, Guzmán-Bracho C, Magos C, 1992. Seroepidemiología de la enfermedad de Chagas en México. Salud Publica Mex 34 :186–196.
-
25
Guzmán-Bracho C, García L, Verdugo JF, Guerrero S, Torres M, Ramírez C, Veslcao-Castrejón O, 1998. Riesgo de transmisión de Tripanosoma cruzi por transfusión de sangre en México. Rev Panam Salud Publica 4 :94–99.
-
26
UNDP/World Bank/WHO, 1997. Special Programme for Research and Training in Tropical Diseases. TDR News 52 :2.
-
27
Zavala-Velázquez JE, 2003. La enfermdad de Chagas en el Estado de Yucatán,México. Rev Biomed 14 :35–43.
-
28
Camargo ME, Segura EL, Kagan IG, Pacheco-Souza JM, Carvalheiro JR, Yanovsky JF, 1987. Normalización del diagnóstico sexológico de la enfermedad de Chagas en Las Américas: evaluación de tres años de colaboración. Bol Oficina Sanit Panam 102 :449–463.
-
29
Sousa-Jurado F, Zumaquero JL, Reyes PA, Cruz-García A, Guzmán-Bracho C, Monteón VM, 2004. Factores bióticos y abióticos que determinan la seroprevalencia de anticuerpos contra Trypanosoma cruzi en el municipio de Bravo, Puebla, México. Salud Publica Mex 46 :39–48.
-
30
Secretaria de Salud, PROY-NOM-032-SSA2, 2000. Para la Vigilancia, Prevención y Control de Enfermedades Transmitidas por Vector. Mexico City, Mexico: Diario Oficial de la Federación, November 21, 2000. Second sección.
-
31
Ramos-Echeverría AA, Monteon-Padilla VM, Reyes PA, 1993. Detección de anticuerpos contra Tripanosoma cruzi en donadores de sangre. Salud Publica Mex 35 :1–9.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1365 | 1295 | 317 |
| Full Text Views | 298 | 11 | 2 |
| PDF Downloads | 68 | 13 | 2 |