HIGH PREVALENCE RATE OF ENTAMOEBA HISTOLYTICA ASYMPTOMATIC INFECTION IN A RURAL MEXICAN COMMUNITY

FERNANDO RAMOS Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by FERNANDO RAMOS in
Current site
Google Scholar
PubMed
Close
,
PATRICIA MORÁN Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by PATRICIA MORÁN in
Current site
Google Scholar
PubMed
Close
,
ENRIQUE GONZÁLEZ Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by ENRIQUE GONZÁLEZ in
Current site
Google Scholar
PubMed
Close
,
GABRIELA GARCÍA Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by GABRIELA GARCÍA in
Current site
Google Scholar
PubMed
Close
,
MANUEL RAMIRO Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by MANUEL RAMIRO in
Current site
Google Scholar
PubMed
Close
,
ALEJANDRO GÓMEZ Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by ALEJANDRO GÓMEZ in
Current site
Google Scholar
PubMed
Close
,
MARÍA DEL CARMEN GARCÍA DE LEÓN Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by MARÍA DEL CARMEN GARCÍA DE LEÓN in
Current site
Google Scholar
PubMed
Close
,
EMMA I. MELENDRO Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by EMMA I. MELENDRO in
Current site
Google Scholar
PubMed
Close
,
ALICIA VALADEZ Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by ALICIA VALADEZ in
Current site
Google Scholar
PubMed
Close
, and
CECILIA XIMÉNEZ Departamento de Medicina Experimental (Department of Experimental Medicine), Facultad de Medicina (School of Medicine), UNAM (National Autonomous University of Mexico), Mexico Distrito Federal, Mexico; Hospital Regional 1° de Octubre (October 1st Regional Hospital), ISSSTE, Mexico Distrito Federal, Mexico; Coordinación de Investigación en Salud (Health Research Coordination), Centro Médico Siglo XXI (21st Century Nacional Medical Center), IMSS (Mexican Institute of Social Security), Mexico Distrito Federal, Mexico

Search for other papers by CECILIA XIMÉNEZ in
Current site
Google Scholar
PubMed
Close
Restricted access

The frequency of Entamoeba histolytica and Entamoeba dispar infection was analyzed in a rural community in the state of Morelos, Mexico, using polymerase chain reaction (PCR). Sociodemographic variables as risk factors for the infection were assessed. Results highlighted the number of individuals with intestinal parasites (43.1%) in the community, indicating extensive fecalism. A high frequency of E. histolytica asymptomatic infection, higher than E. dispar infection (13.8% versus 9.6%), was detected by PCR. Anti-amebic antibody levels (IgG) in serum and saliva (IgA) samples were not associated with E. histolytica intestinal infection. These findings suggest a predominant distribution of E. histolytica strains of low invasive potential in this community.

Author Notes

Reprint requests: Dra. Cecilia Ximénez, Departamento de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis 148, Col. Doctores, CP 06726, México D.F., México. Telephone: +5255-5623 2666, Fax: +5255-5623 2669, E-mail: cximenez@servidor.unam.mx.
  • 1

    Caballero-Salcedo A, Viveros-Rogel M, Salvatierra B, Tapia-Conyer R, Sepúlveda-Amor J, Gutiérrez G, Ortíz-Ortíz L, 1994. Seroepidemiology of amebiasis in Mexico. Am J Trop Med Hyg 50 :412–419.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2

    World Health Organization, 1997. World Health Organization/ Pan American Health Organization/UNESCO report of a consultation of experts on amoebiasis. Wkly Epidemiol Rec WHO 72 :97–99.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3

    Diamond LS, Clark CG, 1993. A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J Eukariot Microbiol 40 :340–344.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4

    Braga L, Gomes ML, Da Silva MW, Façanha FE, Fiuza L, Mann BJ, 2001. Household epidemiology of Entamoeba histolytica infection in an urban community in Northeastern Brazil. Am J Trop Med Hyg 65 :268–271.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5

    Haque R, Ali IM, Petri WA, 1999. Prevalence and immunce response to Entamoeba histolytic infection in preschool children in Bangladesh. Am J Trop Med Hyg 60 :1031–1034.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6

    Gathiram V, Jackson TF, 1987. A longitudinal study of asymptomatic carriers of pathogenic zymodemes of Entamoeba histolytica. S Afr Med J 72 :669–672.

  • 7

    Ramos F, Valdez E, Morán P, González E, Padilla G, Gómez A, Ramiro M, Melendro EI, Muñoz O, Clark G, Ximénez C, 2000. Prevalence of Entamoeba histolytica and Entamoeba dispar in a highly endemic rural population. Arch Med Res 31 :S34–S35.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8

    Ash RL, Orihel TC, 1987. Collection and preservation of faeces. American Society of Clinical Pathologists, eds. Parasites: A Guide to Laboratory Procedures and Identification. Chicago, IL: ASCP Press, 5–14.

    • PubMed
    • Export Citation
  • 9

    Acuña-Soto R, Samuelson J, De Girolami P, Zárate L, Millan-Velasco F, Schoolmick G, Wirth D, 1993. Application of the polymerase chain reaction to the epidemiology of pathogenic and non-pathogenic Entamoeba histolytica. Am J Trop Med Hyg 48 :58–70.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10

    Clark CG, 1993. PCR detection of pathogenic Entamoeba histolytica and differentiation from other intestinal protozoa by riboprinting. Persing C, Smith TE, White TJ, eds. Diagnostic Molecular Microbiology. Washington, DC: ASM Press, 468–474.

    • PubMed
    • Export Citation
  • 11

    Clark GC, Diamond LS, 1992. Differentiation of pathogenic Entamoeba histolytica from other intestinal protozoa by ribo-printing. Arch Med Res 23 :15–16.

  • 12

    Ayeh-Kumi PF, Ali IM, Lockhart LA, Gilchrist CA, Petri WA Jr, Haque R, 2001. Entamoeba histolytica: Genetic diversity of clinical isolates from Bangladesh as demostrated by polymorphisms in the serine-rich gene. Ex Parasitol 99 :80–88.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13

    Valenzuela O, Ramos F, Morán P, González E, Valadez A, Gómez A, Melendro EI, Ramiro M, Muñoz O, Ximénez C, 2001. Persistence of secretory antiamoebic antibodies in patients with invasive intestinal or hepatic amoebiasis. Parasitol Res 87 :849–852.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14

    Okazaki Y, Sumi Y, Niimi A, Furuta H, Sawaki Y, Ueda M, Kaneda T, 1991. Circadian changes of secretory IgA concentration in saliva. M Tsuchiya M, Nagura H, Hibi T, Moro I, eds. Frontiers of Mucosal Immunology, Vol. 1. Amsterdam: Excerta Medica, 263–266.

    • PubMed
    • Export Citation
  • 15

    Kleinbaum DG, Kupper LL, Morgenster H, 1982. Measures of potential impact and summary of the measures. Kleinbaun DG, eds. Epidemiological Research, Principles and Quantitative Methods. New York: Van Nostrand Reinhold, 159–180.

    • PubMed
    • Export Citation
  • 16

    Secretaria de Salud, 2001. Epidemiologia. Información epidemiológica de morbilidad 2000. Mexico D.F., Secretaría de Salud: 15–32.

    • PubMed
    • Export Citation
  • 17

    Walsh JA, 1986. Problems in recognition and diagnosis of amoebiasis: estimations of the global magnitude of morbidity and mortality. Rev Infect Dis 8 :228–238.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18

    Tsutsumi V, Ramírez-Rosales A, Lanz-Mendoza H, Shibayama M, Chávez B, Rangel-López E, Martínez-Palomo A, 1992. Entamoeba histolytica: erythrophagocytosis, collagenolysis, and liver abscess production as virulence markers. Trans R Soc Trop Med Hyg 86 :170–172.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 19

    Muñoz ML, Rojkind M, Calderon C, 1984. Entamoeba histolytica: collagenolytic activity and virulence. J Protozool 31 :468–470.

  • 20

    Braga L, Mendonca Y, Paiva CA, Sales A, Cavalcante AL, Man BJ, 1998. Seropositivity for and intestinal colonization with Entamoeba histolytica and Entamoeba dispar in individuals in Northeastern Brazil. J Clin Microbiol 36 :3044–3045.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 21

    Abd-Alla MD, Ravdin JI, 2002. Diagnosis of amoebic colitis by antigen capture ELISA in patients presenting with acute diarrhoea in Cairo, Egypt. Trop Med Int Health 7 :365–370.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 22

    Abb-Alla MD, Jackson T, Reddy S, Ravdin J, 2000. Diagnosis of invasive amoebiasis by enzyme linked immunosorbent assay of saliva to detect amoebic lecitin antigen and anti-lectin immunoglobulin G antibodies. J Clin Microbiol 38 :2344–2347.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 23

    Del Muro R, Acosta E, Merino E, Glender W, Ortiz-Ortiz L, 1990. Diagnosis of intestinal amebiasis using salivary IgA antibody detection. JID 162 :1360–1365.

  • 24

    Escandon RC, Gomez HJL, Cabral JS, Hernandez RJM, Escobedo J, Olvera JA, 1997. Detection of anti-Entamoeba histolytica IgA salivary antibodies: evaluation of its diagnostic capability in a rural setting. Arch Med Res 28 :S306–S308.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 25

    Haque R, Duggal P, Ali IM, Hossain MB, Mondal D, Sack RB, Farr BM, Beaty TH, Petri WA, 2002. Innate and acquired resistance to amebiasis in Bangladeshi children. J Inf Dis 186 :547–552.

    • PubMed
    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 160 149 6
Full Text Views 307 2 2
PDF Downloads 90 1 1
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save