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-
1
Watt G, Parola P, 2003. Scrub typhus and tropical rickettsioses. Curr Opin Infect Dis 16 :429–436.
-
2
Rosenberg R, 1997. Drug resistant scrub typhus: paradigm and paradox. Parasitol Today 13 :131–132.
-
3
Watt G, Strickman D, Kantipong P, Jongsakul K, Paxton H, 1998. Performance of a dot blot immunoassay for the rapid diagnosis of scrub typhus in a longitudinal case series. J Infect Dis 177 :800–802.
-
4
Coleman RE, Sangkasuwan V, Suwanabun N, Ching W-M, Satabongkot J, Eamsila C, Richards AL, Rowland D, Devine P, Lerdthusnee K, 2002. Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. Am J Trop Med Hyg 67 :497–503.
-
5
Murai K, Tachibana N, Okayama A, Shishime E, Tsuda K, Oshikawa T, 1992. Sensitivity of polymerase chain reaction assay for Rickettsia tsutsugamushi in patients’ blood samples. Microbiol Immunol 36 :1145–1153.
-
6
Manosroi J, Chitipongvivate S, Auwanit W, Manosroi A, 2003. Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. Southeast Asian J Trop Med Public Health 34 :831–838.
-
7
Saisongkorh W, Chenchittikul M, Silpapojakul K, 2004. Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin. Trans Roy Soc Trop Med Hyg 98 :360–366.
-
8
Jiang J, Chan T-C, Temenak JJ, Dasch GA, Ching W-M, Richards AL, 2004. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi.Am J Trop Med Hyg 70 :351–356.
-
9
Watt G, Kantipong P, Jongsakul K, Watcharapichat P, Phulsuksombati D, Strickman D, 2000. Doxycycline and rifampicin for mild scrub-typhus infections in northern Thailand: a randomized trial. Lancet 356 :1057–1061.
-
10
Watt G, Chouriyagune C, Ruangweerayud R, Watcharapichat P, Phulsuksombati D, Jongsakul K, Teja-Isavadharm P, Bhodhi-datta D, Corcoran KD, Dasch GA, Strickman D, 1996. Scrub typhus infections poorly responsive to antibiotics in northern Thailand. Lancet 348 :86–89.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 329 | 290 | 23 |
| Full Text Views | 332 | 4 | 1 |
| PDF Downloads | 112 | 5 | 1 |
DETECTION OF ORIENTIA TSUTSUGAMUSHI IN CLINICAL SAMPLES BY QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION
TASAWAN SINGHSILARAK
TASAWAN SINGHSILARAK
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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WATTANA LEOWATTANA
WATTANA LEOWATTANA
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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SORNCHAI LOOAREESUWAN
SORNCHAI LOOAREESUWAN
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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VAREE WONGCHOTIGUL
VAREE WONGCHOTIGUL
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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JU JIANG
JU JIANG
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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ALLEN L. RICHARDS
ALLEN L. RICHARDS
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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GEORGE WATT
GEORGE WATT
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland; Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand and Department of Medicine, Hawaii AIDS Clinical Research Program, University of Hawaii, Manoa, Honolulu, Hawaii
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Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher’s two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/μL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection.
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-
1
Watt G, Parola P, 2003. Scrub typhus and tropical rickettsioses. Curr Opin Infect Dis 16 :429–436.
-
2
Rosenberg R, 1997. Drug resistant scrub typhus: paradigm and paradox. Parasitol Today 13 :131–132.
-
3
Watt G, Strickman D, Kantipong P, Jongsakul K, Paxton H, 1998. Performance of a dot blot immunoassay for the rapid diagnosis of scrub typhus in a longitudinal case series. J Infect Dis 177 :800–802.
-
4
Coleman RE, Sangkasuwan V, Suwanabun N, Ching W-M, Satabongkot J, Eamsila C, Richards AL, Rowland D, Devine P, Lerdthusnee K, 2002. Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. Am J Trop Med Hyg 67 :497–503.
-
5
Murai K, Tachibana N, Okayama A, Shishime E, Tsuda K, Oshikawa T, 1992. Sensitivity of polymerase chain reaction assay for Rickettsia tsutsugamushi in patients’ blood samples. Microbiol Immunol 36 :1145–1153.
-
6
Manosroi J, Chitipongvivate S, Auwanit W, Manosroi A, 2003. Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. Southeast Asian J Trop Med Public Health 34 :831–838.
-
7
Saisongkorh W, Chenchittikul M, Silpapojakul K, 2004. Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin. Trans Roy Soc Trop Med Hyg 98 :360–366.
-
8
Jiang J, Chan T-C, Temenak JJ, Dasch GA, Ching W-M, Richards AL, 2004. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi.Am J Trop Med Hyg 70 :351–356.
-
9
Watt G, Kantipong P, Jongsakul K, Watcharapichat P, Phulsuksombati D, Strickman D, 2000. Doxycycline and rifampicin for mild scrub-typhus infections in northern Thailand: a randomized trial. Lancet 356 :1057–1061.
-
10
Watt G, Chouriyagune C, Ruangweerayud R, Watcharapichat P, Phulsuksombati D, Jongsakul K, Teja-Isavadharm P, Bhodhi-datta D, Corcoran KD, Dasch GA, Strickman D, 1996. Scrub typhus infections poorly responsive to antibiotics in northern Thailand. Lancet 348 :86–89.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 329 | 290 | 23 |
| Full Text Views | 332 | 4 | 1 |
| PDF Downloads | 112 | 5 | 1 |