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STRONGYLOIDES STERCORALIS RECOMBINANT NIE ANTIGEN SHARES EPITOPE WITH RECOMBINANT Ves v 5 AND Pol a 5 ALLERGENS OF INSECTS

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  • 1 Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York

A new recombinant protein (NIE) for immunodiagnosis of human Strongyloides infection has 13% to 18% amino acid identity with antigen 5 insect venom allergen, but the C-terminal segment of NIE showed highest identity with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as bands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay.

Author Notes

Reprint requests: Franklin A. Neva, LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, Telephone: 301-496-2486, Fax: 301-402-0079, E-mail: fneva@niaid.nih.gov.
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