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1
Ravi V, Ramachandran S, Thompson RW, Andersen JF, Neva FA, 2002. Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3 stage larvae. Mol Biochem Parasitol 125 :73–81.
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2
King TP, 1996. Immunochemical studies of stinging insect venom antigens. Toxicon 34 :1455–1458.
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3
Mizuki N, Sarapata DE, Maria-Sanz JA, Kasahara M, 1992. The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis and sequence similarity to two non-mammalian genes. Mamm Genome 3 :274–280.
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4
Murphy EV, Zhang Y, Zhu W, Biggs J, 1995. The human glioma pathogenesis–related protein is structurally related to plant pathogenesis–related proteins and its gene is expressed specifically in brain tumors. Gene 159 :131–135.
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5
Cornelissen B, Hooft von HR, Van Loon L, Bol I, 1986. Molecular characterization of messenger RNAs for pathogenesis related protein 1a, 1b, 1c produced by TMV infection of tobacco. EMBO J 5 :37–40.
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6
Hawdon JM, Jones BF, Hoffman DR, Hotez PJ, 1996. Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae. J Biol Chem 271 :6672–6678.
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7
Neva FA, Gam AA, Maxwell C, Pelletier LL, 2001. Skin test antigens for immediate hypersensitivity prepared from infective larvae of Strongyloides stercoralis. Am J Trop Med Hyg 65 :567–572.
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8
Lu G, Villalba M, Cosca MR, Hoffman DR, King TP, 1993. Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornet, wasp, and yellow jackets. J Immunol 150 :2823–2830.
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9
Monsalve RI, Lu G, King TP, 1999. Expression of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis) in bacteria or yeast. Protein Expr Purif 16 :410–416.
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10
Laemmli UK, 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227 :680–685.
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11
Cutt JR, Dixon DC, Carr JP, Klessig DF, 1988. Isolation and nucleotide sequence of cDNA clones for the pathogenesis-related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc induced by TMV infection. Nucleic Acids Res 16 :9861.
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12
King TP, Lu G, 1997. Hornet venom allergen antigen 5, Dol m 5: its T cell epitopes in mice and its antigenic crossreactivity with a mammalian testis protein. J Allergy Clin Immunol 99 :630–639.
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13
Berzofsky JA, Berkower IJ, 1984. Antigen-antibody interaction. Paul WE, ed. Fundamental Immunology. New York: Raven Press, 595–644.
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STRONGYLOIDES STERCORALIS RECOMBINANT NIE ANTIGEN SHARES EPITOPE WITH RECOMBINANT Ves v 5 AND Pol a 5 ALLERGENS OF INSECTS
VARATHARAJALU RAVI
VARATHARAJALU RAVI
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York
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TE PIAO KING
TE PIAO KING
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York
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JOHN F. ANDERSEN
JOHN F. ANDERSEN
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York
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THOMAS B. NUTMAN
THOMAS B. NUTMAN
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York
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FRANKLIN A. NEVA
FRANKLIN A. NEVA
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland; Rockefeller University, New York, New York
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A new recombinant protein (NIE) for immunodiagnosis of human Strongyloides infection has 13% to 18% amino acid identity with antigen 5 insect venom allergen, but the C-terminal segment of NIE showed highest identity with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as bands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
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-
1
Ravi V, Ramachandran S, Thompson RW, Andersen JF, Neva FA, 2002. Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3 stage larvae. Mol Biochem Parasitol 125 :73–81.
-
2
King TP, 1996. Immunochemical studies of stinging insect venom antigens. Toxicon 34 :1455–1458.
-
3
Mizuki N, Sarapata DE, Maria-Sanz JA, Kasahara M, 1992. The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis and sequence similarity to two non-mammalian genes. Mamm Genome 3 :274–280.
-
4
Murphy EV, Zhang Y, Zhu W, Biggs J, 1995. The human glioma pathogenesis–related protein is structurally related to plant pathogenesis–related proteins and its gene is expressed specifically in brain tumors. Gene 159 :131–135.
-
5
Cornelissen B, Hooft von HR, Van Loon L, Bol I, 1986. Molecular characterization of messenger RNAs for pathogenesis related protein 1a, 1b, 1c produced by TMV infection of tobacco. EMBO J 5 :37–40.
-
6
Hawdon JM, Jones BF, Hoffman DR, Hotez PJ, 1996. Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae. J Biol Chem 271 :6672–6678.
-
7
Neva FA, Gam AA, Maxwell C, Pelletier LL, 2001. Skin test antigens for immediate hypersensitivity prepared from infective larvae of Strongyloides stercoralis. Am J Trop Med Hyg 65 :567–572.
-
8
Lu G, Villalba M, Cosca MR, Hoffman DR, King TP, 1993. Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornet, wasp, and yellow jackets. J Immunol 150 :2823–2830.
-
9
Monsalve RI, Lu G, King TP, 1999. Expression of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis) in bacteria or yeast. Protein Expr Purif 16 :410–416.
-
10
Laemmli UK, 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227 :680–685.
-
11
Cutt JR, Dixon DC, Carr JP, Klessig DF, 1988. Isolation and nucleotide sequence of cDNA clones for the pathogenesis-related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc induced by TMV infection. Nucleic Acids Res 16 :9861.
-
12
King TP, Lu G, 1997. Hornet venom allergen antigen 5, Dol m 5: its T cell epitopes in mice and its antigenic crossreactivity with a mammalian testis protein. J Allergy Clin Immunol 99 :630–639.
-
13
Berzofsky JA, Berkower IJ, 1984. Antigen-antibody interaction. Paul WE, ed. Fundamental Immunology. New York: Raven Press, 595–644.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 457 | 392 | 33 |
| Full Text Views | 252 | 6 | 0 |
| PDF Downloads | 78 | 5 | 0 |