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CHARACTERIZATION OF DENGUE-2 VIRUS BINDING TO SURFACES OF MAMMALIAN AND INSECT CELLS

BUTSAYA K. THAISOMBOONSUKDepartment of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Microbiology, Mahidol University, Bangkok, Thailand

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EDWARD T. CLAYSONDepartment of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Microbiology, Mahidol University, Bangkok, Thailand

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SOMSAK PANTUWATANADepartment of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Microbiology, Mahidol University, Bangkok, Thailand

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DAVID W. VAUGHNDepartment of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Microbiology, Mahidol University, Bangkok, Thailand

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TIMOTHY P. ENDYDepartment of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Microbiology, Mahidol University, Bangkok, Thailand

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The binding of dengue type 2 (DEN-2) virus to mammalian (LLC-MK2 and Vero) and mosquito (C6/36 and AP61) cell surfaces was investigated by a virus-binding assay using purified 3H-labeled DEN-2 virus. The DEN-2 virus binding to all four cell types was specific and saturable, indicating the presence of a single class of receptors (ranging from 3.7 × 103 to 3.5 × 104 receptors/cell) with a high affinity for DEN-2 virus (Kd ranging from 98 to 171 pM). Treatment of cell surfaces with certain glycosidases significantly reduced virus binding to mammalian cell lines, but not to the insect cell lines examined. Furthermore, heparin was found to compete with mammalian cell receptors for binding to DEN-2 virus and to inhibit viral infection of mammalian cells, but heparin had no effect on viral binding to or infection of insect cells. These results confirm previous reports suggesting that DEN-2 virus receptors on mammalian cell lines are different from those on insect cell lines.

Author Notes

Reprint requests: Butsaya K. Thaisomboonsuk, Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, APO AP 96546 (from the United States) or 315/6 Rajvithi Road, Bangkok 10400, Thailand (from outside the United States).
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