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APPLICATION OF SYNTHETIC 8-KD AND RECOMBINANT GP50 ANTIGENS IN THE DIAGNOSIS OF NEUROCYSTICERCOSIS BY ENZYME-LINKED IMMUNOSORBENT ASSAY

EDNEIA C. BUENOFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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CHRISTINA M. SCHEELFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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ADELAIDE J. VAZFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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LUIS R. MACHADOFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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JOSÉ A. LIVRAMENTOFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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OSVALDO M. TAKAYANAGUIFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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VICTOR C. W. TSANGFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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KATHY HANCOCKFaculty of Pharmacy, University of the Vale do Itajaí, Itajaí, Santa Catarina, Brazil; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Faculty of Pharmacy, and Faculty of Medicine, University of São Paulo, São Paulo, Brazil; Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format.

Author Notes

Reprint requests: Edneia C. Bueno, Curso de Farmácia, Universidade do Vale do Itajaí, Rua Uruguai, 458, Bloco 17, Sala 205, Itajaí, SC, 88.302-202, Brazil, Telephone: 55-47-341-7833, Fax: 55-47-341-7501, E-mail: ecbueno@ccs.univali.br.
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