• 1

    Fidock DA, Nomura T, Talley AK, Cooper RA, Dzekunov SM, Ferdig MT, Ursos LM, Sidhu AB, Naude B, Deitsch KW, Su XZ, Wootton JC, Roepe PD, Wellems TE, 2000. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Mol Cell 6 :861–871.

    • Search Google Scholar
    • Export Citation
  • 2

    Djimde A, Doumbo OK, Cortese JF, Kayentao K, Doumbo S, Diourte Y, Dicko A, Su XZ, Nomura T, Fidock DA, Wellems TE, Plowe CV, Coulibaly D, 2001. A molecular marker for chloroquine-resistant falciparum malaria. N Engl J Med 344 :257–263.

    • Search Google Scholar
    • Export Citation
  • 3

    Djimde A, Doumbo OK, Steketee RW, Plowe CV, 2001. Application of a molecular marker for surveillance of chloroquine-resistant falciparum malaria. Lancet 358 :890–891.

    • Search Google Scholar
    • Export Citation
  • 4

    Dorsey G, Kamya MR, Ndeezi G, Babirye JN, Phares CR, Olson JE, Katabira ET, Rosenthal PJ, 2000. Predictors of chloroquine treatment failure in children and adults with falciparum malaria in Kampala, Uganda. Am J Trop Med Hyg 62 :686–692.

    • Search Google Scholar
    • Export Citation
  • 5

    Zucker JR, Ruebush TK 2nd, Obonyo C, Otieno J, Campbell CC, 2003. The mortality consequences of the continued use of chloroquine in Africa: experience in Siaya, western Kenya. Am J Trop Med Hyg 68 :386–390.

    • Search Google Scholar
    • Export Citation
  • 6

    Muller O, Becher H, van Zweeden AB, Ye Y, Diallo DA, Konate AT, Gbangou A, Kouyate B, Garenne M, 2001. Effect of zinc supplementation on malaria and other causes of morbidity in west African children: randomised double blind placebo controlled trial. BMJ 322 :1567.

    • Search Google Scholar
    • Export Citation
  • 7

    Muller O, Traore C, Kouyate B, 2003. Clinical efficacy of chloroquine in young children with uncomplicated falciparum malaria–a community-based study in rural Burkina Faso. Trop Med Int Health 8 :202–203.

    • Search Google Scholar
    • Export Citation
  • 8

    World Health Organization, 2000. Severe falciparum malaria. World Health Organization, Communicable Diseases Cluster. Trans R Soc Trop Med Hyg 94 (Suppl 1):S1–90.

    • Search Google Scholar
    • Export Citation
  • 9

    Kalter HD, Burnham G, Kolstad PR, Hossain M, Schillinger JA, Khan NZ, Saha S, de Wit V, Kenya-Mugisha N, Schwartz B, Black RE, 1997. Evaluation of clinical signs to diagnose anaemia in Uganda and Bangladesh, in areas with and without malaria. Bull World Health Organ 75 (Suppl 1):103–111.

    • Search Google Scholar
    • Export Citation
  • 10

    Zucker JR, Perkins BA, Jafari H, Otieno J, Obonyo C, Campbell CC, 1997. Clinical signs for the recognition of children with moderate or severe anaemia in western Kenya. Bull World Health Organ 75 (Suppl 1):97–102.

    • Search Google Scholar
    • Export Citation
  • 11

    Luby SP, Kazembe PN, Redd SC, Ziba C, Nwanyanwu OC, Hightower AW, Franco C, Chitsulo L, Wirima JJ, Olivar MA, 1995. Using clinical signs to diagnose anaemia in African children. Bull World Health Organ 73 :477–482.

    • Search Google Scholar
    • Export Citation
  • 12

    Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52 :565–568.

    • Search Google Scholar
    • Export Citation
  • 13

    Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61 :315–320.

    • Search Google Scholar
    • Export Citation
  • 14

    Ranford-Cartwright LC, Taylor J, Umasunthar T, Taylor LH, Babiker HA, Lell B, Schmidt-Ott JR, Lehman LG, Walliker D, Kremsner PG, 1997. Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. Trans R Soc Trop Med Hyg 91 :719–724.

    • Search Google Scholar
    • Export Citation
  • 15

    World Health Organization, 2003. Africa Malaria Report 2003. Geneva: World Health Organization.

  • 16

    Smith T, Beck HP, Kitua A, Mwankusye S, Felger I, Fraser-Hurt N, Irion A, Alonso P, Teuscher T, Tanner M, 1999. Age dependence of the multiplicity of Plasmodium falciparum infections and of other malariological indices in an area of high endemicity. Trans R Soc Trop Med Hyg 93 (Suppl 1):15–20.

    • Search Google Scholar
    • Export Citation
  • 17

    Mayor A, Saute F, Aponte JJ, Almeda J, Gomez-Olive FX, Dgedge M, Alonso PL, 2003. Plasmodium falciparum multiple infections in Mozambique, its relation to other malariological indices and to prospective risk of malaria morbidity. Trop Med Int Health 8 :3–11.

    • Search Google Scholar
    • Export Citation
  • 18

    Mayor AG, Gomez-Olive X, Aponte JJ, Casimiro S, Mabunda S, Dgedge M, Barreto A, Alonso PL, 2001. Prevalence of the K76T mutation in the putative Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene and its relation to chloroquine resistance in Mozambique. J Infect Dis 183 :1413–1416.

    • Search Google Scholar
    • Export Citation
  • 19

    Dorsey G, Kamya MR, Singh A, Rosenthal PJ, 2001. Polymorphisms in the Plasmodium falciparum pfcrt and pfmdr-1 genes and clinical response to chloroquine in Kampala, Uganda. J Infect Dis 183 :1417–1420.

    • Search Google Scholar
    • Export Citation
  • 20

    Genton B, al-Yaman F, Beck HP, Hii J, Mellor S, Narara A, Gibson N, Smith T, Alpers MP, 1995. The epidemiology of malaria in the Wosera area, East Sepik Province, Papua New Guinea, in preparation for vaccine trials. I. Malariometric indices and immunity. Ann Trop Med Parasitol 89 :359–376.

    • Search Google Scholar
    • Export Citation
  • 21

    Ekvall H, Premji Z, Bjorkman A, 1998. Chloroquine treatment for uncomplicated childhood malaria in an area with drug resistance: early treatment failure aggravates anaemia. Trans R Soc Trop Med Hyg 92 :556–560.

    • Search Google Scholar
    • Export Citation
  • 22

    May J, Meyer CG, 2003. Association of Plasmodium falciparum chloroquine resistance transporter variant T76 with age-related plasma chloroquine levels. Am J Trop Med Hyg 68 :143–146.

    • Search Google Scholar
    • Export Citation
  • 23

    Plowe CV, Doumbo OK, Djimde A, Kayentao K, Diourte Y, Doumbo SN, Coulibaly D, Thera M, Wellems TE, Diallo DA, 2001. Chloroquine treatment of uncomplicated Plasmodium falciparum malaria in Mali: parasitologic resistance versus therapeutic efficacy. Am J Trop Med Hyg 64 :242–246.

    • Search Google Scholar
    • Export Citation
  • 24

    Kublin JG, Cortese JF, Njunju EM, Mukadam RA, Wirima JJ, Kazembe PN, Djimde AA, Kouriba B, Taylor TE, Plowe CV, 2003. Reemergence of chloroquine-sensitive Plasmodium falciparum malaria after cessation of chloroquine use in Malawi. J Infect Dis 187 :1870–1875.

    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 172 60 5
PDF Downloads 23 12 1
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

 

ASSOCIATION OF PLASMODIUM FALCIPARUM ISOLATES ENCODING THE P. FALCIPARUM CHLOROQUINE RESISTANCE TRANSPORTER GENE K76T POLYMORPHISM WITH ANEMIA AND SPLENOMEGALY, BUT NOT WITH MULTIPLE INFECTIONS

INAS Z. ABDEL-AZIZHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by INAS Z. ABDEL-AZIZ in
Current site
Google Scholar
PubMed
Close
,
NADJA OSTERHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by NADJA OSTER in
Current site
Google Scholar
PubMed
Close
,
AUGUST STICHHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by AUGUST STICH in
Current site
Google Scholar
PubMed
Close
,
BOUBACAR COULIBALYHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by BOUBACAR COULIBALY in
Current site
Google Scholar
PubMed
Close
,
WENDYAM A. GUIGEMDÉHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by WENDYAM A. GUIGEMDÉ in
Current site
Google Scholar
PubMed
Close
,
HANNES WICKERTHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by HANNES WICKERT in
Current site
Google Scholar
PubMed
Close
,
KATHY T. ANDREWSHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by KATHY T. ANDREWS in
Current site
Google Scholar
PubMed
Close
,
BOCAR KOUYATÉHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by BOCAR KOUYATÉ in
Current site
Google Scholar
PubMed
Close
, and
MICHAEL LANZERHygiene Institut, Abteilung Parasitologie, Universitätsklinikum Heidelberg, Heidelberg, Germany; Centre de Recherche en Santé à Nouna, Nouna, Burkina Faso

Search for other papers by MICHAEL LANZER in
Current site
Google Scholar
PubMed
Close
Restricted access

The aim of the study was to assess whether infections with Plasmodium falciparum isolates encoding the P. falciparum chloroquine resistance transporter (pfcrt) gene K76T polymorphism, a molecular marker for chloroquine resistance, are associated with multiple infections, age, or clinical signs of malaria in a semi-immune population in a holoendemic area of Burkina Faso. The parameters of interest were investigated in 210 P. falciparum-positive inhabitants. Logistic regression analysis showed that pfcrt K76T-carrying isolates are significantly more likely to cause anemia and splenomegaly. Furthermore, we found that infections with P. falciparum isolates encoding pfcrt K76T are dependent on age rather than multiple infections. Our findings suggest that pfcrt K76T might serve as a valuable marker for assessing the long-term clinical effect of chronic infections with chloroquine-resistant P. falciparum isolates in populations, without the need of drug efficacy trials.

Save