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1
Guthrie R, Susi A, 1963. A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants. Pediatrics 32 :338–343.
-
2
Lindstrom B, Ericsson O, Alvan G, Rombo L, Ekman L, Rais M, Sjoqvist F, 1985. Determination of chloroquine and its desethyl metabolite in whole blood: an application for samples collected in capillary tubes and dried on filter paper. Ther Drug Monit 7 :207–210.
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3
Prior TW, Highsmith WE, Friedman KJ Jr, Perry TR, Scheuerbrandt G, Silverman LM, 1990. A model for molecular screening of newborns: simultaneous detection of Duchenne/Becker muscular dystrophies and cystic fibrosis. Clin Chem 36 :1756–1759.
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4
Farnert A, Arez AP, Correia AT, Bjorkman A, Snounou G, do Rosario V, 1999. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction. Trans R Soc Trop Med Hyg 93 :50–53.
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5
Long GW, Fries L, Watt GH, Hoffman SL, 1995. Polymerase chain reaction amplification from Plasmodium falciparum on dried blood spots. Am J Trop Med Hyg 52 :344–346.
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6
Gil JP, Nogueira F, Stromberg-Norklit J, Lindberg J, Carrolo M, Casimiro C, Lopes D, Arez AP, Cravo PV, Rosario VE, 2003. Detection of atovaquone and Malarone resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb). Mol Cell Probes 17 :85–89.
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7
Kain KC, Lanar DE, 1991. Determination of genetic variation within Plasmodium falciparum by using enzymatically amplified DNA from filter paper disks impregnated with whole blood. J Clin Microbiol 29 :1171–1174.
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8
Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52 :565–568.
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9
Snounou G, Zhu X, Siripoon N, Jarra W, Thaithong S, Brown KN, Viriyakosol S, 1999. Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans R Soc Trop Med Hyg 93 :369–374.
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10
Farnert A, Arez AP, Babiker HA, Beck HP, Benito A, Bjorkman A, Bruce MC, Conway DJ, Day KP, Henning L, Mercereau-Puijalon O, Ranford-Cartwright LC, Rubio JM, Snounou G, Walliker D, Zwetyenga J, do Rosario VE, 2001. Genotyping of Plasmodium falciparum infections by PCR: a comparative multicentre study. Trans R Soc Trop Med Hyg 95 :225–232.
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11
Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61 :315–320.
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12
Henning L, Felger I, Beck HP, 1999. Rapid DNA extraction for molecular epidemiological studies of malaria. Acta Trop 72 :149–155.
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SHORT REPORT: RAPID DNA EXTRACTION FROM ARCHIVE BLOOD SPOTS ON FILTER PAPER FOR GENOTYPING OF PLASMODIUM FALCIPARUM
SÁNDOR BERECZKY
SÁNDOR BERECZKY
Infectious Diseases Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Emergency Medicine Unit, Department of Medicine, Kullbergska Hospital, Katrineholm, Sweden
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ANDREAS MÅRTENSSON
ANDREAS MÅRTENSSON
Infectious Diseases Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Emergency Medicine Unit, Department of Medicine, Kullbergska Hospital, Katrineholm, Sweden
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J. PEDRO GIL
J. PEDRO GIL
Infectious Diseases Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Emergency Medicine Unit, Department of Medicine, Kullbergska Hospital, Katrineholm, Sweden
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ANNA FÄRNERT
ANNA FÄRNERT
Infectious Diseases Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Emergency Medicine Unit, Department of Medicine, Kullbergska Hospital, Katrineholm, Sweden
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The practical advantages of sampling and storing blood on filter paper for analyses of human and pathogen genes highlight the need for reliable, sensitive, and cost-effective DNA extraction methods. We describe a new Tris-EDTA (TE) buffer-based method for extraction of DNA from blood dried on filter paper. The method was evaluated against the commonly used methanol and Chelex® methods, regarding polymerase chain reaction detection of Plasmodium falciparum parasites from samples stored for 1–2 years. The sensitivity of detection was dependent on the parasite density and type of filter paper. For 3MM® Whatman filter paper, the sensitivity was 100%, 73%, and 93% for the TE, methanol, and Chelex® methods, respectively. For the longer stored 903® Schleicher & Schuell filter paper, the sensitivity was 93%, 73%, and 0%, respectively. This rapid, simple, and inexpensive extraction method generated superior results from archived specimens compared with the two standard methods and may represent a useful tool in molecular epidemiologic studies.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Guthrie R, Susi A, 1963. A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants. Pediatrics 32 :338–343.
-
2
Lindstrom B, Ericsson O, Alvan G, Rombo L, Ekman L, Rais M, Sjoqvist F, 1985. Determination of chloroquine and its desethyl metabolite in whole blood: an application for samples collected in capillary tubes and dried on filter paper. Ther Drug Monit 7 :207–210.
-
3
Prior TW, Highsmith WE, Friedman KJ Jr, Perry TR, Scheuerbrandt G, Silverman LM, 1990. A model for molecular screening of newborns: simultaneous detection of Duchenne/Becker muscular dystrophies and cystic fibrosis. Clin Chem 36 :1756–1759.
-
4
Farnert A, Arez AP, Correia AT, Bjorkman A, Snounou G, do Rosario V, 1999. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction. Trans R Soc Trop Med Hyg 93 :50–53.
-
5
Long GW, Fries L, Watt GH, Hoffman SL, 1995. Polymerase chain reaction amplification from Plasmodium falciparum on dried blood spots. Am J Trop Med Hyg 52 :344–346.
-
6
Gil JP, Nogueira F, Stromberg-Norklit J, Lindberg J, Carrolo M, Casimiro C, Lopes D, Arez AP, Cravo PV, Rosario VE, 2003. Detection of atovaquone and Malarone resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb). Mol Cell Probes 17 :85–89.
-
7
Kain KC, Lanar DE, 1991. Determination of genetic variation within Plasmodium falciparum by using enzymatically amplified DNA from filter paper disks impregnated with whole blood. J Clin Microbiol 29 :1171–1174.
-
8
Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52 :565–568.
-
9
Snounou G, Zhu X, Siripoon N, Jarra W, Thaithong S, Brown KN, Viriyakosol S, 1999. Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans R Soc Trop Med Hyg 93 :369–374.
-
10
Farnert A, Arez AP, Babiker HA, Beck HP, Benito A, Bjorkman A, Bruce MC, Conway DJ, Day KP, Henning L, Mercereau-Puijalon O, Ranford-Cartwright LC, Rubio JM, Snounou G, Walliker D, Zwetyenga J, do Rosario VE, 2001. Genotyping of Plasmodium falciparum infections by PCR: a comparative multicentre study. Trans R Soc Trop Med Hyg 95 :225–232.
-
11
Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61 :315–320.
-
12
Henning L, Felger I, Beck HP, 1999. Rapid DNA extraction for molecular epidemiological studies of malaria. Acta Trop 72 :149–155.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 496 | 257 | 26 |
| Full Text Views | 648 | 15 | 5 |
| PDF Downloads | 482 | 14 | 0 |