ALLELIC DIMORPHISM IN THE MEROZOITE SURFACE PROTEIN-3α IN KOREAN ISOLATES OF PLASMODIUM VIVAX

EUN-TAEK HAN Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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TAE-EUI SONG Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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JAE-HWAN PARK Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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EUN-HEE SHIN Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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SANG-MEE GUK Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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TAE-YUN KIM Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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JONG-YIL CHAI Department of Parasitology, Kangwon National University College of Medicine, Chunchon, Korea; Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea; Institute of Malariology and Department of Parasitology, Inje University College of Medicine, Pusan, Korea

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To study the genetic diversity of re-emerging Plasmodium vivax in the Republic of Korea, nucleotide sequence variations at the merozoite surface protein-3α (PvMSP-3α) locus were analyzed using 24 re-emerging isolates and 4 isolates from imported cases. Compared with the well known Belem strain (Brazil), a large number of amino acid substitutions, deletions, and insertions were found at the locus of the isolates examined. The Korean isolates were divided into two allelic types; type I (15 isolates), similar to the Belem strain, and type II (9), similar to the Chess strain (New Guinea). Isolates from imported cases were classified into three types; type III (1 from Malaysia), similar to type B from western Thailand, type IV (1 each from Indonesia and India), and type V (1 from Pakistan), both being new types. Our results have shown that the MSP-3α locus of re-emerging Korean P. vivax is dimorphic with two allelic types coexisting in the endemic area.

Author Notes

Reprint requests: Jong-Yil Chai, Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center, Seoul 110-799, Korea, E-mail: cjy@snu.ac.kr.
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