SHORT REPORT: EVALUATION OF A SIMPLE AND INEXPENSIVE PHOTOMETRIC DEVICE FOR THE MEASUREMENT OF HEMOGLOBIN

STEFFEN BORRMANN Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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SUNNY OYAKHIROME Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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GILBERT ESSER Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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CORDULA TRINKLE Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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SAADOU ISSIFOU Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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MARTIN P. GROBUSCH Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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SANJEEV KRISHNA Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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PETER G. KREMSNER Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany; Department of Cellular and Molecular Medicine, Infectious Diseases, St. George’s Hospital Medical School, London, United Kingdom

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DOI:
https://doi.org/10.4269/ajtmh.2004.71.691
Volume/Issue:
Volume 71: Issue 5
Page(s):
691–692
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We have evaluated the accuracy of a simple and inexpensive photometric device (DHT) for the estimation of the blood concentration of hemoglobin by comparison with an automated, high-resolution, flow cytometry-based hematology analyzer (CellDyn 3000) and a centrifugal quantitative buffy coat hematology system (QBC I). We have analyzed the hemoglobin values of 163 individual blood samples. Bland-Altman analysis showed that the methods agreed only poorly: mean differences were 1.0 g/dL with limits of agreement (LOA) of −1.2 g/dL to 3.2 g/dL for the comparison of DHT and CellDyn measurements, 0.5 g/dL with LOA of -2.0 g/dL to 3.0 g/dL for the comparison of DHT with QBC measurements, and 0.5 g/dL with LOA of −1.1 g/dL to 2.1 g/dL for the comparison of QBC with CellDyn measurements. We conclude that the poor agreement of the DHT with the CellDyn does not permit the use of the DHT for critical hemoglobin measurements, particularly in transfusion services.

Author Notes

Copyright:
Copyright 2004 The American Society of Tropical Medicine 2004
Received:
31 Mar 2004
|
Accepted:
28 May 2004
|
Published Online:
Nov 2004
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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