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-
1
Garcia HH, Gilman R, Martinez M, Tsang VC, Pilcher JB, Herrera G, Diaz F, Alvarado M, Miranda E, 1993. Cysticercosis as a major cause of epilepsy in Peru. The Cysticercosis Working Group in Peru (CWG). Lancet 341 :197–200.
-
2
Garcia HH, Del Brutto OH, 2000. Taenia solium cysticercosis. Infect Dis Clin North Am 14 :97–119.
-
3
Gonzalez AE, Gilman R, Garcia HH, McDonald J, Kacena K, Tsang VC, Pilcher JB, Suarez F, Gavidia C, Miranda E, 1994. Use of sentinel pigs to monitor environmental Taenia solium contamination. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 51 :847–850.
-
4
Gonzalez AE, Verastegui M, Noh JC, Gavidia C, Falcon N, Bernal T, Garcia HH, Tsang VC, Gilman RH, Wilkins PP, 1999. Persistence of passively transferred antibodies in porcine Taenia solium cysticercosis. Cysticercosis Working Group in Peru. Vet Parasitol 86 :113–118.
-
5
Gonzalez AE, Gavidia C, Falcon N, Bernal T, Verastegui M, Garcia HH, Gilman RH, Tsang VC, Cysticercosis Working Group in Peru, 2001. Protection of pigs with cysticercosis from further infections after treatment with oxfendazole. Am J Trop Med Hyg 65 :15–18.
-
6
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
7
Hancock K, Tsang VCW, 1986. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni.J Immunol Methods 92 :167–176.
-
8
Richards FO, Tsang VCW, Brand JA, Hancock K, 1989. Modification of the FAST-ELISA for field diagnosis of Schistosomiasis mansoni with serum of blood samples. Trop Med Parasitol 40 :332–334.
-
9
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99 :257–261.
-
10
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86 :1001–1007.
-
11
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133 :115–124.
-
12
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Noh J, Tsang V, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41 :2577–2586.
-
13
Garcia HH, Gilman RH, Catacora M, Verastegui M, Gonzalez AE, Tsang VC, 1997. Serologic evolution of neurocysticercosis patients after antiparasitic therapy. J Infect Dis 175 :486–489.
-
14
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 :32–35.
-
15
Elandt-Johnson RC, 1999. Survival Models and Data Analysis. New York: John Wiley & Sons, Inc.
-
16
Xiao BG, Link H, 1998. Immune regulation within the central nervous system. J Neurol Sci 157 :1–12.
-
17
Garcia HH, Gonzalez AE, Gilman RH, Palacios LG, Jimenez I, Rodriguez S, Verastegui M, Wilkins P, Tsang VC; Cysticercosis Working Group in Peru, 2001. Short report: transient antibody response in Taenia solium infection in field conditions-a major contributor to high seroprevalence. Am J Trop Med Hyg 65 :31–32.
-
18
Garcia HH, Herrera G, Gilman RH, Tsang VC, Pilcher JB, Diaz JF, Candy EJ, Miranda E, Naranjo J, 1994. Discrepancies between cerebral computed tomography and Western blot in the diagnosis of neurocysticercosis. The Cysticercosis Working Group in Peru (Clinical Studies Coordination Board). Am J Trop Med Hyg 50 :152–157.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 753 | 723 | 30 |
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PORCINE ANTIBODY RESPONSES TO TAENIA SOLIUM ANTIGENS RGP50 AND STS18VAR1
SUKWAN HANDALI
SUKWAN HANDALI
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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ARMANDO E. GONZALEZ
ARMANDO E. GONZALEZ
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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KATHY HANCOCK
KATHY HANCOCK
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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HECTOR H. GARCIA
HECTOR H. GARCIA
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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JACQUELIN M. ROBERTS
JACQUELIN M. ROBERTS
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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ROBERT H. GILMAN
ROBERT H. GILMAN
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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VICTOR C. W. TSANG
VICTOR C. W. TSANG
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurológicas, Lima, Peru; Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland
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Cysticercosis, a disease caused by the larval form of Taenia solium, is diagnosed by detection of specific antibodies or by imaging techniques. Our preferred immunologic assay for cysticercosis is the enzyme-linked immunoelectrodifusion transfer blot, or immunoblot, using the lentil lectin bound antigens from larval cysts. Antibody reactivity with any one of seven glycoproteins is diagnostic for cysticercosis. To develop a simple antibody detection assay for field use, we have synthesized an 8-kD diagnostic antigen, sTs18var1 (a secreted protein with a mature size of 67 amino acids), and expressed a 50-kD membrane protein antigen, rGp50. We used these two diagnostic proteins in a quantitative Falcon assay screening test–enzyme-linked immunosorbent assay (FAST-ELISA) to measure the antibody responses in Peruvian pigs with cysticercosis. Three study designs were used. First, we followed the kinetics of antibody responses against these two diagnostic proteins in pigs with cysticercosis that were treated with oxfendazole. Second, we measured antibody response in naive experimentally infected pigs. Third, we followed the maternal antibodies against rGp50 and sTs18var1 in piglets born from sows with cysticercosis. These studies showed that antibody responses against the two diagnostic proteins in the FAST-ELISA are quantitatively correlated with infection by viable cysts, with anti-sTs18var1 activity being most responsive to the status of infection.
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-
1
Garcia HH, Gilman R, Martinez M, Tsang VC, Pilcher JB, Herrera G, Diaz F, Alvarado M, Miranda E, 1993. Cysticercosis as a major cause of epilepsy in Peru. The Cysticercosis Working Group in Peru (CWG). Lancet 341 :197–200.
-
2
Garcia HH, Del Brutto OH, 2000. Taenia solium cysticercosis. Infect Dis Clin North Am 14 :97–119.
-
3
Gonzalez AE, Gilman R, Garcia HH, McDonald J, Kacena K, Tsang VC, Pilcher JB, Suarez F, Gavidia C, Miranda E, 1994. Use of sentinel pigs to monitor environmental Taenia solium contamination. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 51 :847–850.
-
4
Gonzalez AE, Verastegui M, Noh JC, Gavidia C, Falcon N, Bernal T, Garcia HH, Tsang VC, Gilman RH, Wilkins PP, 1999. Persistence of passively transferred antibodies in porcine Taenia solium cysticercosis. Cysticercosis Working Group in Peru. Vet Parasitol 86 :113–118.
-
5
Gonzalez AE, Gavidia C, Falcon N, Bernal T, Verastegui M, Garcia HH, Gilman RH, Tsang VC, Cysticercosis Working Group in Peru, 2001. Protection of pigs with cysticercosis from further infections after treatment with oxfendazole. Am J Trop Med Hyg 65 :15–18.
-
6
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
7
Hancock K, Tsang VCW, 1986. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni.J Immunol Methods 92 :167–176.
-
8
Richards FO, Tsang VCW, Brand JA, Hancock K, 1989. Modification of the FAST-ELISA for field diagnosis of Schistosomiasis mansoni with serum of blood samples. Trop Med Parasitol 40 :332–334.
-
9
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99 :257–261.
-
10
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86 :1001–1007.
-
11
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133 :115–124.
-
12
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Noh J, Tsang V, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41 :2577–2586.
-
13
Garcia HH, Gilman RH, Catacora M, Verastegui M, Gonzalez AE, Tsang VC, 1997. Serologic evolution of neurocysticercosis patients after antiparasitic therapy. J Infect Dis 175 :486–489.
-
14
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 :32–35.
-
15
Elandt-Johnson RC, 1999. Survival Models and Data Analysis. New York: John Wiley & Sons, Inc.
-
16
Xiao BG, Link H, 1998. Immune regulation within the central nervous system. J Neurol Sci 157 :1–12.
-
17
Garcia HH, Gonzalez AE, Gilman RH, Palacios LG, Jimenez I, Rodriguez S, Verastegui M, Wilkins P, Tsang VC; Cysticercosis Working Group in Peru, 2001. Short report: transient antibody response in Taenia solium infection in field conditions-a major contributor to high seroprevalence. Am J Trop Med Hyg 65 :31–32.
-
18
Garcia HH, Herrera G, Gilman RH, Tsang VC, Pilcher JB, Diaz JF, Candy EJ, Miranda E, Naranjo J, 1994. Discrepancies between cerebral computed tomography and Western blot in the diagnosis of neurocysticercosis. The Cysticercosis Working Group in Peru (Clinical Studies Coordination Board). Am J Trop Med Hyg 50 :152–157.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 753 | 723 | 30 |
| Full Text Views | 250 | 3 | 1 |
| PDF Downloads | 55 | 3 | 1 |