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1
Young EJ, 1994. An overview of human brucellosis. Clin Infect Dis 21 :283–290.
-
2
Daddod WA, Abdulia ZA, 2000. A panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis. East Mediterr Health J 6 :304–312.
-
3
Diaz R, Maravi-Poma E, Rivero A, 1976. Comparison of counter-immunoelectrophoresis with other serological tests in the diagnosis of human brucellosis. Bull World Health Organ 53 :417–424.
-
4
Renoux G, Plommet M, Philippon A, 1971. Microreactions d’agglutination et the fixation de complement pour le diagnostics des brucelloses. Ann Rech Vet 2 :263–269.
-
5
Spink WW, McCullough NB, Hutchings LM, Mingle CK, 1954. A standardized antigen and agglutination technic for human brucellosis. Am J Clin Pathol 24 :496–498.
-
6
Al Dahouk S, Tomaso H, Nockler K, Neubauer H, Frangoulidis D, 2003. Laboratory-based diagnosis of brucellosis-a review of the literature. Part II: serological tests for brucellosis. Clin Lab 49 :577–589.
-
7
Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R, 2003. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for the serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 10 :1141–1146.
-
8
Esel D, Doganay M, Alp E, Sumerkan B, 2003. Prospective evaluation of blood cultures in a Turkish university hospital: epidemiology, microbiology and patient outcome. Clin Microbiol Infect 9 :1038–1044.
-
9
Caksen H, Arslan S, Oner AF, Cesur Y, Ceylan A, Atas B, Abuhandan M, 2002. Childhood brucellosis is still a severe problem in the eastern region of Turkey. Trop Doct 32 :91–92.
-
10
Akdeniz H, Irmak H, Demiroz AP, 1998. Evaluation of brucellosis cases in Van region of wastern Anatolia: A-3 year experience. Nagoya Med J 42 :101–110.
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11
Akdeniz H, Irmak H, Seckinli T, Buzgan T, Demirö z AP, 1998. Hematological manifestations in .brucellosis cases in Turkey. Acta Med Okayama 52 :63–65.
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12
Gedikoglu S, Helvaci S, Ozakin C, Gokirmak F, Kilicturgay K, 1996. Detection of Brucella melitensis by BACTEC NR 730 and BACTEC 9120 systems. Eur J Epidemiol 12 :649–650.
-
13
Mert A, Ozaras R, Tabak F, Bilir M, Yilmaz M, Kurt C, Ongoren S, Tanriverdi M, Ozturk R, 2003. The sensitivity and specificity of Brucella agglutination tests. Diagn Microbiol Infect Dis 46 :241–243.
-
14
Memish ZA, Almuneef M, Mah MW, Qassem LA, Osoba AO, 2002. Comparison of the Brucella standard agglutination test with the ELISA IgG and IgM in patients with Brucella bacteremia. Diagn Microbiol Infect Dis 44 :129–132.
-
15
Ceylan E, Irmak H, Buzgan T, Karahocagil MK, Evirgen O, Sakarya N, Akdeniz H, Demiroz AP, 2003. Van iline bagli bazi koylerde insan ve hayvan populasyonunda bruselloz seroprevalansi. Van Tip Dergisi 10 :1–5.
-
16
Thakur SD, Kumar R, Thapliyal DC, 2002. Human brucellosis: review of an under-diagnosed animal transmitted disease. J Commun Dis 34 :287–301.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 18 | 18 | 7 |
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| PDF Downloads | 113 | 24 | 0 |
USE OF THE BRUCELLA IgM AND IgG FLOW ASSAYS IN THE SERODIAGNOSIS OF HUMAN BRUCELLOSIS IN AN AREA ENDEMIC FOR BRUCELLOSIS
HASAN IRMAK
HASAN IRMAK
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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TURAN BUZGAN
TURAN BUZGAN
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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ÖMER EVIRGEN
ÖMER EVIRGEN
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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HAYRETTIN AKDENIZ
HAYRETTIN AKDENIZ
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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A. PEKCAN DEMIROZ
A. PEKCAN DEMIROZ
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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THERESIA H. ABDOEL
THERESIA H. ABDOEL
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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HENK L. SMITS
HENK L. SMITS
Department of Infectious Diseases and Clinical Microbiology, Yüzüncü Yil University, Van, Turkey; KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen, Amsterdam, The Netherlands
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The clinical utility of two complementary tests for brucellosis, the Brucella IgM and IgG flow assays, was evaluated in a hospital in eastern Turkey. The results show that the flow assays are convenient diagnostic tests for use in endemic areas. A positive result in the flow assays was obtained in 91% and 97% of the admission sera from adult and pediatric patients with brucellosis, respectively, and the sensitivity at admission was 100% for culture-confirmed brucellosis. The assay system performed equally well in diagnosing patients at different stages of illness including patients with acute, subacute, or chronic disease and with relapse. The results of the flow assays correlated well with those of a serum agglutination test at a cut-off ≥1:160. The agreement was 92%. Application of the flow assays on serum samples collected during a village survey for brucellosis after an outbreak demonstrated their diagnostic potential as field tests.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Young EJ, 1994. An overview of human brucellosis. Clin Infect Dis 21 :283–290.
-
2
Daddod WA, Abdulia ZA, 2000. A panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis. East Mediterr Health J 6 :304–312.
-
3
Diaz R, Maravi-Poma E, Rivero A, 1976. Comparison of counter-immunoelectrophoresis with other serological tests in the diagnosis of human brucellosis. Bull World Health Organ 53 :417–424.
-
4
Renoux G, Plommet M, Philippon A, 1971. Microreactions d’agglutination et the fixation de complement pour le diagnostics des brucelloses. Ann Rech Vet 2 :263–269.
-
5
Spink WW, McCullough NB, Hutchings LM, Mingle CK, 1954. A standardized antigen and agglutination technic for human brucellosis. Am J Clin Pathol 24 :496–498.
-
6
Al Dahouk S, Tomaso H, Nockler K, Neubauer H, Frangoulidis D, 2003. Laboratory-based diagnosis of brucellosis-a review of the literature. Part II: serological tests for brucellosis. Clin Lab 49 :577–589.
-
7
Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R, 2003. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for the serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 10 :1141–1146.
-
8
Esel D, Doganay M, Alp E, Sumerkan B, 2003. Prospective evaluation of blood cultures in a Turkish university hospital: epidemiology, microbiology and patient outcome. Clin Microbiol Infect 9 :1038–1044.
-
9
Caksen H, Arslan S, Oner AF, Cesur Y, Ceylan A, Atas B, Abuhandan M, 2002. Childhood brucellosis is still a severe problem in the eastern region of Turkey. Trop Doct 32 :91–92.
-
10
Akdeniz H, Irmak H, Demiroz AP, 1998. Evaluation of brucellosis cases in Van region of wastern Anatolia: A-3 year experience. Nagoya Med J 42 :101–110.
-
11
Akdeniz H, Irmak H, Seckinli T, Buzgan T, Demirö z AP, 1998. Hematological manifestations in .brucellosis cases in Turkey. Acta Med Okayama 52 :63–65.
-
12
Gedikoglu S, Helvaci S, Ozakin C, Gokirmak F, Kilicturgay K, 1996. Detection of Brucella melitensis by BACTEC NR 730 and BACTEC 9120 systems. Eur J Epidemiol 12 :649–650.
-
13
Mert A, Ozaras R, Tabak F, Bilir M, Yilmaz M, Kurt C, Ongoren S, Tanriverdi M, Ozturk R, 2003. The sensitivity and specificity of Brucella agglutination tests. Diagn Microbiol Infect Dis 46 :241–243.
-
14
Memish ZA, Almuneef M, Mah MW, Qassem LA, Osoba AO, 2002. Comparison of the Brucella standard agglutination test with the ELISA IgG and IgM in patients with Brucella bacteremia. Diagn Microbiol Infect Dis 44 :129–132.
-
15
Ceylan E, Irmak H, Buzgan T, Karahocagil MK, Evirgen O, Sakarya N, Akdeniz H, Demiroz AP, 2003. Van iline bagli bazi koylerde insan ve hayvan populasyonunda bruselloz seroprevalansi. Van Tip Dergisi 10 :1–5.
-
16
Thakur SD, Kumar R, Thapliyal DC, 2002. Human brucellosis: review of an under-diagnosed animal transmitted disease. J Commun Dis 34 :287–301.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 18 | 18 | 7 |
| Full Text Views | 326 | 82 | 1 |
| PDF Downloads | 113 | 24 | 0 |