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Acedo Sánchez C, Martín Sánchez J, Vélez Bernal ID, Sanchís Marín MC, Louassini M, Maldonado JA, Morillas Márquez F, 1996. Leishmaniasis eco-epidemiology in the Alpujarra region (Granada province, southern Spain). Int J Parasitol 25 :303–310.
-
2
Gradoni L, Scalone A, Gramiccia M, Troiani M, 1996. Epidemiological surveillance of leishmaniasis in HIV-1-infected individuals in Italy. AIDS 10 :785–791.
-
3
Pineda JA, Macías J, Morillas F, Fernández-Ochoa J, Cara J, de la Rosa R, Mira JA, Martín-Sánchez J, González M, Delgado J, Acedo C, Lissen E, 2001. Evidence of increased risk for Leishmania infantum infection among HIV-seronegative intravenous drug users from southern Spain. Eur J Clin Microbiol Infect Dis 20 :354–357.
-
4
Amela C, López-Gay D, Alberdi JC, Castilla J, 1996. Injecting drug use as risk factor for visceral leishmaniasis in AIDS patients. Eur J Epidemiol 12 :91–92.
-
5
Cruz I, Morales MA, Noguer I, Rodríguez A, Alvar J, 2002. Leishmania in discarded syringes from intravenous drug users. Lancet 359 :1124–1125.
-
6
Morillas-Márquez F, Martín-Sánchez J, Acedo-Sánchez C, Pineda JA, Macías J, Sanjuan-García J, 2002. Leishmania infantum (Protozoa, Kinetoplastida): transmission from infected patients to experimental animal under conditions that simulate needle-sharing. Exp Parasitol 100 :71–74.
-
7
World Health Organization, 2000. Leishmania/HIV-Coinfection, South-Western Europe, 1990–1998: Retrospective Analysis of 965 Cases. Geneva: World Health Organization. WHO/LEISH/2000.42.
-
8
de la Rosa R, Pineda JA, Delgado J, Macías J, Morillas F, Mira JA, Sánchez-Quijano A, Leal M, Lissen E, 2002. Incidence of and risk factors for symptomatic visceral leishmaniasis among human immunodeficiency virus type-1 infected patients from Spain in the era of highly active antiretroviral therapy. J Clin Microbiol 40 :762–767.
-
9
le Fichoux I, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol 37 :1953–1957.
-
10
Otero AC, da Silva VO, Luz KG, Palatnik M, Pirmez C, Fernandez C, Palatnik de Sousa CB, 2000. Occurrence of Leishmania donovani DNA in donated blood from seroreactive Brazilian blood donors. Am J Trop Med Hyg 62 :128–131.
-
11
Costa CH, Stewart JM, Gomes RB, Garcez LM, Ramos PK, Bozza M, Satoskar A, Dissanayake S, Santos RS, Silva MR, Shaw JJ, David JR, Maguire JH, 2002. Asymptomatic human carriers of Leishmania chagasi.Am J Trop Med Hyg 66 :334–337.
-
12
Martín-Sánchez J, López-López MC, Acedo-Sánchez C, Castro-Fajardo JJ, Pineda JA, Morillas-Márquez F, 2001. Diagnosis of infections with Leishmania infantum using PCR-ELISA. Parasitology 122 :607–615.
-
13
Martín-Sánchez J, Pineda JA, Andreu-López M, Delgado J, Macías J, de la Rosa R, Morillas-Márquez F, 2002. The high sensitivity of a PCR-ELISA in the diagnosis of cutaneous and visceral leishmaniasis caused by Leishmania infantum.Ann Trop Med Parasitol 96 :669–677.
-
14
Alter HJ, Conry-Cantilena C, Melpolder J, Tan D, Van Raden M, Herion D, Lau D, Hoofnagle JH, 1997. Hepatitis C in asymptomatic blood donors. Hepatology 26 (Suppl 1):29S–33S.
-
15
Iqbal J, Hira PR, Saroj G, Philip R, Al-Ali F, Madda PJ, Sher A, 2002. Imported visceral leishmaniasis: diagnostics dilemmas and comparative analysis of three assays. J Clin Microbiol 40 :475–479.
-
16
Kar K, 1995. Serodiagnosis of leishmaniasis. Crit Rev Microbiol 21 :123–152.
-
17
López-Vélez R, Turientes MC, Gómez-Mampaso E, 1998. Reacciones cruzadas de la serología a leishmania infantum por inmunofluorescencia indirecta en pacientes HIV+ y HIV nega-tivos con tuberculosis activa. Enferm Infecc Microbiol Clin 16 :130–131.
-
18
Molina R, Lohse JM, Pulido F, Laguna F, López-Vélez R, Alvar J, 1999. Infection of sand flies by humans coinfected with Leishmania infantum and human immunodeficiency virus. Am J Trop Med Hyg 60 :51–53.
-
19
Nigro L, Vinci C, Romano F, Russo R, 1996. Comparison of the indirect immunofluorescent antibody test and the direct agglutination test for serodiagnosis of visceral leishmaniasis in HIV-infected subjects. Eur J Clin Microbiol Infect Dis 15 :832–835.
-
20
Piarroux R, Gambarelli F, Dumon H, Fontes M, Dunan S, Mary C, Toga B, Qulici M, 1994. Comparison of PCR with direct examination of bone marrow aspiration, myeloculture and serology for diagnosis of visceral leishmaniasis in immunocom-promised patients. J Clin Microbiol 32 :746–749.
-
21
Dereure J, Lanotte G, Pratlong F, Gouvernet J, Majhour J, Belazzoug S, Khiami A, Rageh HA, Jarry D, Perieres J, Rioux JA, 1998. Canine leishmaniasis from Leishmania infantum: value and production of the latex test. Ecoepidemiologic applications. Bull Soc Pathol Exot 91 :300–305.
-
22
Badaro R, Jones TC, Lorenço R, Cerf BJ, Sampaio D, Carvalho EM, Rocha H, Texeira R, Johnson WD, 1986. A prospective study of visceral leishmaniasis in an endemic area of Brazil. J Infect Dis 154 :640–649.
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DETECTION OF LEISHMANIA INFANTUM KINETOPLAST DNA IN PERIPHERAL BLOOD FROM ASYMPTOMATIC INDIVIDUALS AT RISK FOR PARENTERALLY TRANSMITTED INFECTIONS: RELATIONSHIP BETWEEN POLYMERASE CHAIN REACTION RESULTS AND OTHER LEISHMANIA INFECTION MARKERS
Individuals with frequent exposures to agents spread through the parenteral route show a high prevalence of Leishmania seropositivity in Spain. However, the frequency of positive polymerase chain reaction (PCR) results for Leishmania in blood in this setting remains unknown. In this study, L. infantum kinetoplast DNA (kDNA) was found in blood from 23 (24%) of 95 asymptomatic individuals with a serum Leishmania antibody titer ≥ 1:20 and in none of 44 seronegative individuals. The greater the antibody titer, the greater the proportion of PCR-positive samples, but 16 (20%) of 81 individuals with antibody titers ≤ 1:40 tested positive by PCR. Nine (37%) PCR-positive and 22 (19%) (P = 0.03) PCR-negative individuals showed a positive leishmanin skin test result. This results show that a remarkable proportion of asymptomatic Leishmania-seropositive individuals at risk for parenterally transmitted infections carry Leishmania kDNA in blood. This is more common in subjects with a high serum Leishmania antibody level and a positive leishmanin skin test result.