• 1

    Schantz PM, 1991. Parasitic zoonoses in perspective. Int J Parasitol 321 :161–170.

  • 2

    Gottstein B, Reichen J, 2003. Echinococcosis/hydatidosis. Cook GC, Zumla A, eds. Manson’s Tropical Diseases. London: W. B. Saunders, Elsevier Science Ltd., 1561–1582.

  • 3

    Babba H, Messedi A, Masmoudi S, Zribi M, Grillot R, Ambriose-Thomas P, Beyrouti I, Sahnoun Y, 1994. Diagnosis of human hydatidosis: comparison between imagery and six serologic techniques. Am J Trop Med Hyg 50 :64–68.

    • Search Google Scholar
    • Export Citation
  • 4

    WHO/OIE, 2001. Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS, eds. Geneva: World Health Organization.

  • 5

    Richards KS, Morris DL, 1990. Effect of albendazole on human hydatid cysts: an ultrastructural study. HPB Surg. 2 :105–112.

  • 6

    Moskopp D, Lotterer E, 1993. Concentrations of albendazole in serum, cerebrospinal fluid and hydatidous brain cyst. Neurosurg Rev 16 :35–37.

    • Search Google Scholar
    • Export Citation
  • 7

    Morris DL, Richards KS, Chinnery JB, 1986. Protoscolicidal effect of praziquantel—in-vitro and electron microscopical studies on Echinococcus granulosus.J Antimicrob Chemother 18 :687–691.

    • Search Google Scholar
    • Export Citation
  • 8

    Taylor DH, Morris DL, 1989. Combination chemotherapy is more effective in postspillage prophylaxis for hydatid disease than either albendazole or praziquantel alone. Br J Surg 76 :954.

    • Search Google Scholar
    • Export Citation
  • 9

    Boctor FN, Peter JB, 1990. IgG subclasses in human chronic schistosomiasis: over-production of schistosome-specific and non-specific IgG4. Clin Exp Immunol 82 :574–578.

    • Search Google Scholar
    • Export Citation
  • 10

    Short JA, Heiner DC, Hsiao RL, Andersen FL, 1990. Immunoglobulin E and G4 antibodies in cysticercosis. J Clin Microbiol 28 :1635–1639.

  • 11

    Kurniawan A, Yazdanbakhsh M, van Ree R, Aalberse R, Selkirk ME, Partono F, Maizels RM, 1993. Differential expression of IgE and IgG4 specific antibody responses in asymptomatic and chronic human filariasis. J Immunol 150 :3941–3950.

    • Search Google Scholar
    • Export Citation
  • 12

    Daeki AO, Craig PS, Shambesh MK, 2000. IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Ann Trop Med Parasitol 94 :319–328.

    • Search Google Scholar
    • Export Citation
  • 13

    Aceti A, Pennica A, Teggi A, Fondacaro LM, Caferro M, Leri O, Tacchi G, Celestino D, Quaranta G, de Rosa F, Sebastiani A, 1993. IgG subclasses in human hydatid disease: prominence of the IgG4 response. Int Arch Allergy Immunol 102 :347–351.

    • Search Google Scholar
    • Export Citation
  • 14

    Wen H, Craig PS, 1994. Immunoglobulin G subclass responses in human cystic and alveolar echinococcosis. Am J Trop Med Hyg 51 :741–748.

  • 15

    Shambesh MK, Craig PS, Wen H, Rogan MT, Paolillo E, 1997. IgG1 and IgG4 serum antibody responses in asymptomatic and clinically expressed cystic echinococcosis patients. Acta Trop 64 :53–63.

    • Search Google Scholar
    • Export Citation
  • 16

    Ayles HM, Corbett EL, Taylor I, Cowie AG, Bligh J, Walmsley K, Bryceson AD, 2002. A combined medical and surgical approach to hydatid disease: 12 years’ experience at the Hospital for Tropical Diseases, London. Ann R Coll Surg Engl 84 :100–105.

    • Search Google Scholar
    • Export Citation
  • 17

    Filice C, Brunetti E, 1997. Use of PAIR in human cystic echinococcosis. Acta Trop 64 :95–107.

  • 18

    Harlow E, Lane D, 1988. Immunoblotting. Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 471–509.

  • 19

    Rigano R, Profumo E, Ioppolo S, Notargiacomo S, Ortona E, Teggi A, Siracusano A, 1995. Immunological markers indicating the effectiveness of pharmacological treatment in human hydatid disease. Clin Exp Immunol 102 :281–285.

    • Search Google Scholar
    • Export Citation
  • 20

    Ramzy RM, Helmy H, El Zayyat EA, Rifaat MM, Abdel Hameed DM, Abdel-Baki MH, 1999. An enzyme-linked immunosorbent assay for detection of IgG1 antibodies specific to human cystic echinococcosis in Egypt. Trop Med Int Health 4 :616–620.

    • Search Google Scholar
    • Export Citation
  • 21

    McVie A, Ersfeld K, Rogan MT, Craig PS, 1997. Expression and immunological characterisation of Echinococcus granulosus recombinant antigen B for IgG4 subclass detection in human cystic echinococcosis. Acta Trop 67 :19–35.

    • Search Google Scholar
    • Export Citation
  • 22

    Zhang W, Li J, McManus DP, 2003. Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev 16 :18–36.

 
 
 

 

 
 
 

 

 

 

 

 

 

HUMAN CYSTIC ECHINOCOCCOSIS: EVALUATION OF POST-TREATMENT SEROLOGIC FOLLOW-UP BY IgG SUBCLASS ANTIBODY DETECTION

View More View Less
  • 1 Hospital for Tropical Diseases, London, United Kingdom; Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom; Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

Assessment of post-treatment disease activity among patients with cystic echinococcosis (CE) is insensitive using detection of CE-specific total IgG antibody. This study investigated whether serum concentrations of CE-specific IgG subclasses 1–4 correlate better with disease activity than total IgG. We studied a cohort of patients (n = 28) with symptomatic CE treated with anthelminthic drugs and surgery and who were followed up clinically and radiologically for a mean of 5.6 years (range = 3–12 years). Serial archived sera collected during follow-up were retrospectively analyzed by enzyme-linked immunosorbent assays using crude horse hydatid cyst fluid as antigen. Changes in concentrations of antibodies were correlated with clinical and radiologic outcome. At diagnosis, concentrations of CE-specific total IgG, IgG1, and IgG2 antibodies were significantly elevated in a greater proportion of patients compared with IgG3 and IgG4 antibodies. During post-treatment follow up, the IgG2 antibody response provided the best correlate of disease activity.

Save