By H. J. Bensted, W. Bulloch, L. Dudgeon, A. G. Gardner, E. D. W. Greig, D. Harvey, W. F. Harvey, T. J. Mackie, R. A. O'Brien, H. M. Perry, H. Scutze, P. Bruce White, W. J. Wilson. London, 1929. His Majesty's Stationery Office. Pp. 1–482
by A. Trevor Willis, M.D., B.S. (Melb.), Ph.D. (Leeds), M.C.Path., M.C.P.A., Reader in Microbiology, Monash University, formerly Lecturer in Bacteriology, University of Leeds. xiv + 234 pages, illustrated, second edition. Butterworth Inc., Washington. 1965. $8.50
The studies reported here show that the Turlock virus can be isolated in a culture system which utilizes trypsinized chick-embryo cells as the substrate for viral growth. Viral strains isolated by this technique can be rapidly identified in a colorimetric neutralization test based on the use of chick-embryo cells, a single dilution of the viral isolate and a single dilution of a pretested standard immune serum.
A series of 222 mosquito-pool suspensions was tested in parallel in mice and embryonated eggs and in chick-embryo-cell cultures; 12 strains of Turlock virus were recovered. The isolation results suggest that the tissue-culture method used was comparable in sensitivity to the mouse-embryonated-egg host system.