ADAPTATION OF A STRAIN OF PLASMODIUM FALCIPARUM FROM GHANA TO AOTUS LEMURINUS GRISEIMEMBRA, A. NANCYMAI, AND A. VOCIFERANS MONKEYS

JOANN S. SULLIVAN Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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JAMES J. SULLIVAN Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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ALLISON WILLIAMS Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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KATHARINE K. GRADY Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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AMY BOUNNGASENG Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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CURTIS S. HUBER Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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DOUGLAS NACE Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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TYRONE WILLIAMS Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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G. GALE GALLAND Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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JOHN W. BARNWELL Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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WILLIAM E. COLLINS Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Atlanta Research and Education Foundation, Atlanta, Georgia

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A strain of Plasmodium falciparum from Ghana was adapted to Aotus lemurinus griseimembra, A. nancymai, and A. vociferans monkeys. Gametocytes in splenectomized A. nancymai were infective to Anopheles freeborni mosquitoes. Sporozoite transmission was accomplished in two splenectomized A. nancymai with prepatent periods of 22 and 25 days. The Ghana III/CDC strain of P. falciparum is susceptible to treatment with chloroquine and mefloquine.

Author Notes

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    Berzins K, Adams S, Broderson JR, Chizzolini C, Hansson M, Lovgren K, Millet P, Morris CL, Perlmann H, Perlmann P, Sjolander A, Stahl S, Sullivan JS, Troye-Blomberg M, Collins WE, 1995. Immunogenicity in Aotus monkeys of ISCOM formulated repeat sequences from the Plasmodium falciparum asexual blood stage antigen Pf155/RESA. Vaccine Res 4 :121–133.

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    Kumar S, Collins WE, Egan A, Yadava A, Girrard O, Blackman M, Guevara Patino JA, Diggs C, Kaslow D, 2000. Immunogenicity and efficacy in Aotus monkeys of four recombinant Plasmodium falciparum vaccines in multiple adjuvant formulations based on the 19-kD C-terminus of the merozoite surface protein 1 (MSP-1). Infect Immun 68 :2215–2223.

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    Collins WE, Galland GG, Sullivan JS, Morris CL, 1994. Selection of different strains of Plasmodium falciparum for testing blood-stage vaccines in Aotus nancymai monkeys. Am J Trop Med Hyg 51 :224–232.

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