• 1

    Bhatnagar S, Bhan MK, Sommerfelt H, Sazawal S, Kumar R, Saini S, 1993. Enteroaggregative Escherichia coli may be a new pathogen causing acute and persistent diarrhea. Scand J Infect Dis 25 :579–583.

    • Search Google Scholar
    • Export Citation
  • 2

    Gascon J, Vargas M, Quinto L, Corachan M, Jimenez de Anta MT, Vila J, 1998. Enteroaggregative Escherichia coli strains as a cause of traveler’s diarrhea: a case-control study. J Infect Dis 177 :1409–1412.

    • Search Google Scholar
    • Export Citation
  • 3

    Oberhelman RA, Laborde D, Mera R, Starszak E, Saunders P, Mirza A, Bessinger GT, Hull A, 1998. Colonization with enteroadherent, enterotoxigenic and enterohemorrhagic Escherichia coli among day-care center attendees in New Orleans, Louisiana. Pediatr Infect Dis J 17 :1159–1162.

    • Search Google Scholar
    • Export Citation
  • 4

    Presterl E, Nadrchal R, Wolf D, Rotter M, Hirschl AM, 1999. Enteroaggregative and enterotoxigenic Escherichia coli among isolates from patients with diarrhea in Austria. Eur J Clin Microbiol Infect Dis 18 :209–212.

    • Search Google Scholar
    • Export Citation
  • 5

    Savarino SJ, Fasano A, Watson J, Martin BM, Levine MM, Guandalini S, Guerry P, 1993. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E. coli heat-stable toxin. Proc Natl Acad Sci USA 90 :3093–3097.

    • Search Google Scholar
    • Export Citation
  • 6

    Nataro JP, Kaper JB, 1998. Diarrheagenic Escherichia coli. Clin Microbiol Rev 11 :142–201.

  • 7

    Nataro JP, Deng Y, Maneval DR, German AL, Martin WC, Levine MM, 1992. Aggregative adherence fimbriae I of enteroaggregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes. Infect Immun 60 :2297–2304.

    • Search Google Scholar
    • Export Citation
  • 8

    Jiang ZD, Greenberg D, Nataro JP, Steffen R, DuPont HL, 2002. Rate of occurrence and pathogenic effect of enteroaggregative Escherichia coli virulence factors in international travelers. J Clin Microbiol 40 :4185–4190.

    • Search Google Scholar
    • Export Citation
  • 9

    Okeke IN, Lamikanra A, Czeczulin J, Dubovsky F, Kaper JB, Nataro JP, 2000. Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in southwest Nigeria. J Infect Dis 181 :252–260.

    • Search Google Scholar
    • Export Citation
  • 10

    Greenberg DE, Jiang ZD, Steffen R, Verenker MP, DuPont HL, 2002. Markers of inflammation in bacterial diarrhea among travelers, with a focus on enteroaggregative Escherichia coli pathogenicity. J Infect Dis 185 :944–949.

    • Search Google Scholar
    • Export Citation
  • 11

    Czeczulin JR, Whittam TS, Henderson IR, Navarro-Garcia F, Nataro JP, 1999. Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli.Infect Immun 67 :2692–2699.

    • Search Google Scholar
    • Export Citation
  • 12

    Okeke IN, Lamikanra A, Steinruck H, Kaper JB, 2000. Characterization of Escherichia coli strains from cases of childhood diarrhea in provincial southwestern Nigeria. J Clin Microbiol 38 :7–12.

    • Search Google Scholar
    • Export Citation
  • 13

    Bouzari S, Jafari MN, Shokouhi F, Parsi M, Jafari A, 2000. Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli.FEMS Microbiol Lett 185 :89–93.

    • Search Google Scholar
    • Export Citation
  • 14

    Vila J, Gene A, Vargas M, Gascon J, Latorre C, Jimenez de Anta MT, 1998. A case-control study of diarrhoea in children caused by Escherichia coli producing heat-stable enterotoxin (EAST-1). J Med Microbiol 47 :889–891.

    • Search Google Scholar
    • Export Citation
  • 15

    Schmidt H, Knop C, Franke S, Aleksic S, Heesemann J, Karch H, 1995. Development of PCR for screening of enteroaggregative Escherichia coli.J Clin Microbiol 33 :701–705.

    • Search Google Scholar
    • Export Citation
  • 16

    Dutta S, Pal S, Chakrabarti S, Dutta P, Manna B, 1999. Use of PCR to identify enteroaggregative Escherichia coli as an important cause of acute diarrhoea among children living in Calcutta, India. J Med Microbiol 48 :1011–1016.

    • Search Google Scholar
    • Export Citation
  • 17

    Mikhail IA, Fox E, Haberberger RL, Ahmed MH, Abbatte EA, 1990. Epidemiology of bacterial pathogens associated with infectious diarrhea in Djibouti. J Clin Microbiol 28 :956–961.

    • Search Google Scholar
    • Export Citation
  • 18

    Ogunsanya TI, Rotimi VO, Adenuga A, 1994. A study of the aetiological agents of childhood diarrhoea in Lagos, Nigeria. J Med Microbiol 40 :10–14.

    • Search Google Scholar
    • Export Citation
  • 19

    Albert MJ, Faruque AS, Faruque SM, Sack RB, Mahalanabis D, 1999. Case-control study of enteropathogens associated with childhood diarrhea in Dhaka, Bangladesh. J Clin Microbiol 37 :3458–3464.

    • Search Google Scholar
    • Export Citation
  • 20

    Luckner D, Lell B, Greve B, Lehman LG, Schmidt-Ott RJ, Matousek P, Herbich K, Schmid D, Mba R, Kremsner PG, 1998. No influence of socioeconomic factors on severe malarial anaemia, hyperparasitaemia or reinfection. Trans R Soc Trop Med Hyg 92 :478–481.

    • Search Google Scholar
    • Export Citation
  • 21

    Stacy-Phipps S, Mecca JJ, Weiss JB, 1995. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J Clin Microbiol 33 :1054–1059.

    • Search Google Scholar
    • Export Citation
  • 22

    Eslava C, Navarro-Garcia F, Czeczulin JR, Henderson IR, Cravioto A, Nataro JP, 1998. Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli.Infect Immun 66 :3155–3163.

    • Search Google Scholar
    • Export Citation
  • 23

    Vila J, Vargas M, Henderson IR, Gascon J, Nataro JP, 2000. Enteroaggregative Escherichia coli virulence factors in traveler’s diarrhea strains. J Infect Dis 182 :1780–1783.

    • Search Google Scholar
    • Export Citation
  • 24

    Elias WPJ, Czeczulin JR, Henderson IR, Trabulsi LR, Nataro JP, 1999. Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli. J Bacteriol 181 :1779–1785.

    • Search Google Scholar
    • Export Citation
  • 25

    Olsvik O, Strockbine NA, 1993. PCR detection of heat-stable, heat-labile, and shiga-like toxin genes in Escherichia coli. Persing DH, Smith TF, Tenover FC, White TC, eds. Diagnostic Molecular Microbiology. Washington, DC: American Society for Microbiology Press, 271–276.

  • 26

    Franke J, Franke S, Schmidt H, Schwarzkopf A, Wieler LH, Baljer G, Beutin L, Karch H, 1994. Nucleotide sequence analysis of enteropathogenic Escherichia coli (EPEC) adherence factor probe and development of PCR for rapid detection of EPEC harboring virulence plasmids. J Clin Microbiol 32 :2460–2463.

    • Search Google Scholar
    • Export Citation
  • 27

    Schoolnik GK, 1993. PCR detection of Shigella species and enteroinvasive Escherichia coli. Persing DH, Smith HR, Tenover FC, White TC, eds. Diagnostic Molecular Microbiology. Washington, DC: American Society for Microbiology Press, 277–281.

Past two years Past Year Past 30 Days
Abstract Views 1 1 1
Full Text Views 345 127 1
PDF Downloads 54 35 1
 
 
 
 
 
 
 
 
 
 
 

FREQUENCY AND VIRULENCE PROPERTIES OF DIARRHEAGENIC ESCHERICHIA COLI IN CHILDREN WITH DIARRHEA IN GABON

ELISABETH PRESTERLDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by ELISABETH PRESTERL in
Current site
Google Scholar
PubMed
Close
,
RALPH H. ZWICKDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by RALPH H. ZWICK in
Current site
Google Scholar
PubMed
Close
,
SONJA REICHMANNDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by SONJA REICHMANN in
Current site
Google Scholar
PubMed
Close
,
ALEXANDER AICHELBURGDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by ALEXANDER AICHELBURG in
Current site
Google Scholar
PubMed
Close
,
STEFAN WINKLERDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by STEFAN WINKLER in
Current site
Google Scholar
PubMed
Close
,
PETER G. KREMSNERDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by PETER G. KREMSNER in
Current site
Google Scholar
PubMed
Close
, and
WOLFGANG GRANINGERDepartment of Medicine I, Division of Infectious Diseases, University of Vienna, Vienna, Austria; Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon; Department of Parasitology, Institute for Tropical Medicine, University of Tuebingen, Tuebingen, Germany

Search for other papers by WOLFGANG GRANINGER in
Current site
Google Scholar
PubMed
Close
View More View Less
Restricted access

To investigate the presence of diarrheagenic Escherichia coli in Lambaréné, Gabon, 150 children with diarrhea were screened for enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction and an HEp-2 cell culture techniques. Isolates of EAEC were detected in 57 children, two thirds of them between six months and two years of age, and isolates of ETEC were detected in seven patients. Isolates of EPEC, EHEC, and EIEC were not present in this population. Among the EAEC, the pCVD432 plasmid, a heat stable (ST)-like (ST-like) enterotoxin (EAST), and a plasmid-encoded heat-labile toxin (PET) were detected in 19, 34, and 42 cases, respectively. Detection of pCVD432, EAST, and PET were significantly associated with EAEC identified by the HEp-2 cell assay. Although detected only in 16 patients, the presence of the fimbriae AAF I (aagA) and AAF II (aafA) were more likely to occur in EAEC than in non-EAEC (odds ratio [OR] = 4.1, 95% confidence interval [CI] = 0.5–38.6, and OR = 2.3, 95% CI = 1.0–5.3, respectively). The EAEC isolates exhibited decreased susceptibility for ampicillin, tetracycline, and trimethoprim.

Save