• 1

    Monteiro FA, Pérez R, Panzera F, Dujardin JP, Galvão C, Rocha D, Noireau F, Schofield C, Beard CB, 1999. Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma.Mem lnst Oswaldo Cruz Rio J 94 :229–238.

    • Search Google Scholar
    • Export Citation
  • 2

    García BA, 1999. Molecular phylogenetic relationships among species of the genus Triatoma. Carcavallo RU, Galíndez Girón I, Jurberg J, Lent H, eds. Atlas of Chagas’ Disease Vectors in the Americas. Volume III. Rio de Janeiro: Editora Fiocruz, 971–980.

  • 3

    García BA, Powell JR, 1998. Phylogeny of species of Triatoma (Hemiptera: Reduviidae) based on mitochondrial DNA sequences. J Med Entomol 35 :232–238.

    • Search Google Scholar
    • Export Citation
  • 4

    García BA, Moriyama EN, Powell JR, 2001. Mitochondrial DNA sequences of triatomines (Hemiptera: Reduviidae): phylogenetic relationships. J Med Entomol 38 :675–683.

    • Search Google Scholar
    • Export Citation
  • 5

    Weir BS, Cockerham CC, 1984. Estimating F-statistics for the analysis of population structure. Evolution 38 :1358–1370.

  • 6

    Excoffier L, Smouse PE, Quattro JM, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131 :479–491.

    • Search Google Scholar
    • Export Citation
  • 7

    Schneider S, Kueffer JM, Roessli D, Excoffier L, 1997. Arlequin, Version 1.1: A Software for Population Genetic Data Analysis. Geneva, Switzerland: Genetics and Biometry Laboratory, University of Geneva.

  • 8

    Clement M, Posada D, Crandrall KA, 2000. TCS: a computer program to estimate gene genealogies. Mol Ecol 9 :1657–1659.

  • 9

    Nei M, 1987. Molecular Evolutionary Genetics. New York: Columbia University Press.











View More View Less
  • 1 Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, Administración Nacional de Laboratorios e Institutos de Salud, Ministerio de Salud de Argentina, Buenos Aires, Argentina

Mitochondrial DNA sequences of the 12S and 16S ribosomal RNA genes were analyzed in five natural populations of the Chagas’ disease vector Triatoma infestans from Argentina. DNA sequence comparisons of 878 basepairs (12S plus 16S) revealed 13 haplotypes. A total of 10 private haplotypes were found in four of the populations analyzed, suggesting low current levels of genetic exchange. The levels of genetic differentiation between the population of Chancaní (Córdoba) and other two of the populations analyzed indicated significant deviation from a pattern of unrestricted gene flow. The haplotypic diversity and the private haplotypes found in the geographically closest localities of Chancaní and El Jardín (La Rioja) suggest that the reduction in the population size by insecticide treatment did not avoid the recovery of the populations apparently from survivors of the same area.

Author Notes

Reprint requests: Beatriz A. García, CC 35, Suc. 16, 5016 Córdoba, Argentina.