Desjeux P, 1996. Leishmaniasis. Public health aspects and control. Clin Dermatol 14 :417–423.
Pearson RD, de Queiroz SA, 1996. Clinical spectrum of leishmaniasis. Clin Infect Dis 22 :1–13.
Sundar S, More DK, Singh MK, Singh VP, Sharma S, Makharia A, Kumar PC, Murray HW, 2000. Failure of pentavalent antimony in visceral leishmaniasis in India. Report from the center of the Indian epidemic. Clin Infect Dis 31 :1104–1107.
Zijlstra EE, Ali MS, El-Hassan AM, El-Toum IA, Satti M, Gjalib HW, Kager PA, 1992. Kala-zar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg 86 :505–507.
Scott JM, Shreffler WG, Ghalib HW, Asad A, Siddig M, Badaro R, Reed SG, 1991. A rapid and simple diagnostic test for active visceral leishmaniasis. Am J Trop Med Hyg 44 :272–277.
Piarroux R, Gambarelli F, Dumon H, Fontes M, Dunan S, Mary C, Toga B, Quilici M, 1994. Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral Leishmaniasis in immunocompromised patients. J Clin Microbiol 32 :746–749.
Badaró R, Benson D, Eulálio MC, Freire M, Cunha S, Netto EM, Pedral-Sampaio D, Madureira C, Burns JM, Houghton RL, David JR, Reed SG, 1996. A cloned antigen of L. chagasi that predicts active visceral leishmaniasis (VL). J Infect Dis 173 :758–761.
Zijlstra E, Daifalla N, Kager P, Khalil E, El-Hassan, Reed S, Ghalib H, 1998. K39 enzyme-linked immunosorbent assay for diagnosis of Leishmania donovani infection. Clin Diagn Lab Immunol 5 :717–720.
Burns JM Jr, Shreffler WG, Benson DR, Ghalib HW, Badaro′ R, Reed SG, 1993. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects antibody in African and American visceral leishmaniasis. Proc Nat Acad Sci USA 90 :775–779.
Bradford MM, 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 77 :248–254.
Sundar S, Reed S, Singh V, Kumar P, Murray H, 1998. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet 351 :563–565.
Sundar S, Pai K, Sahu M, Kumar V, Murray, HW, 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96 :19–23.
Bern C, Jha SN, Joshi AB, Thakur GD, Bista MB, 2000. Use of the recombinant K39 dipstick test and the direct agglutination test in a setting endemic for visceral leishmaniasis in Nepal. Am J Trop Med Hyg 63 :153–157.
Delgado O, Feliciangeli MD, Coraspe V, Silva S, Perez A, Arias J, 2001. Value of a dipstick based on recombinant rK39 antigen for differential diagnosis of American visceral leishmaniasis from other sympatric endemic diseases in Venezuela. Parasite 8 :355–357.
Zijlstra E, Nur Y, Desjeux P, Khalil E, El-Hassan A, Groen J, 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Med Int Health 6 :108–113.
Jelinek T, Eichenlaub S, Losher T, 1999. Sensitivity and specificity of a rapid immunochromatographic test for diagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis 18 :669–670.
Dietze R, Barros GB, Teixeira L, Harris J, Michelson K, Falqueto A, Corey R, 1997. Effect of eliminating seropositive canines on the transmission of visceral leishmaniasis in Brazil. Clin Infect Dis 25 :1240–1242.
Pintado V, Lopez-Velez R, 2001. HIV-associated visceral leishmaniasis. Clin Microbiol Infect 7 :291–300.
Houghton R, Petrescu M, Benson D, Skeiky Y, Scalone A, Badaro R, Reed S, Gradoni L, 1998. A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type I patients and a prognostic indicator for monitoring patients undergoing drug therapy. J Infect Dis 177 :1339–1344.
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This study evaluated the performance of recombinant K39 (rK39) antigen in a immunochromatographic format (strip test) and a crude antigen enzyme-linked immunosorbent assay in the diagnosis of Brazilian visceral leishmaniasis (VL) in 128 consecutive patients with parasitologically proven infections (by microscopy and/or culture). For each patient, a medical history was obtained and a complete physical examination was performed. Controls included 10 healthy volunteers and 50 patients with other diseases: malaria (10), leprosy (9), Chagas’ disease (10), tuberculosis (10), and cutaneous leishmaniasis (11). The sensitivities of the rK39 antigen strip test and the ELISA were 90% and 89%, respectively, while the specificities were 100% and 98%, respectively. Our study confirms the accuracy of the rK39 antigen strip test in the diagnosis of VL in a high prevalence population.
Desjeux P, 1996. Leishmaniasis. Public health aspects and control. Clin Dermatol 14 :417–423.
Pearson RD, de Queiroz SA, 1996. Clinical spectrum of leishmaniasis. Clin Infect Dis 22 :1–13.
Sundar S, More DK, Singh MK, Singh VP, Sharma S, Makharia A, Kumar PC, Murray HW, 2000. Failure of pentavalent antimony in visceral leishmaniasis in India. Report from the center of the Indian epidemic. Clin Infect Dis 31 :1104–1107.
Zijlstra EE, Ali MS, El-Hassan AM, El-Toum IA, Satti M, Gjalib HW, Kager PA, 1992. Kala-zar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg 86 :505–507.
Scott JM, Shreffler WG, Ghalib HW, Asad A, Siddig M, Badaro R, Reed SG, 1991. A rapid and simple diagnostic test for active visceral leishmaniasis. Am J Trop Med Hyg 44 :272–277.
Piarroux R, Gambarelli F, Dumon H, Fontes M, Dunan S, Mary C, Toga B, Quilici M, 1994. Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral Leishmaniasis in immunocompromised patients. J Clin Microbiol 32 :746–749.
Badaró R, Benson D, Eulálio MC, Freire M, Cunha S, Netto EM, Pedral-Sampaio D, Madureira C, Burns JM, Houghton RL, David JR, Reed SG, 1996. A cloned antigen of L. chagasi that predicts active visceral leishmaniasis (VL). J Infect Dis 173 :758–761.
Zijlstra E, Daifalla N, Kager P, Khalil E, El-Hassan, Reed S, Ghalib H, 1998. K39 enzyme-linked immunosorbent assay for diagnosis of Leishmania donovani infection. Clin Diagn Lab Immunol 5 :717–720.
Burns JM Jr, Shreffler WG, Benson DR, Ghalib HW, Badaro′ R, Reed SG, 1993. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects antibody in African and American visceral leishmaniasis. Proc Nat Acad Sci USA 90 :775–779.
Bradford MM, 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 77 :248–254.
Sundar S, Reed S, Singh V, Kumar P, Murray H, 1998. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet 351 :563–565.
Sundar S, Pai K, Sahu M, Kumar V, Murray, HW, 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96 :19–23.
Bern C, Jha SN, Joshi AB, Thakur GD, Bista MB, 2000. Use of the recombinant K39 dipstick test and the direct agglutination test in a setting endemic for visceral leishmaniasis in Nepal. Am J Trop Med Hyg 63 :153–157.
Delgado O, Feliciangeli MD, Coraspe V, Silva S, Perez A, Arias J, 2001. Value of a dipstick based on recombinant rK39 antigen for differential diagnosis of American visceral leishmaniasis from other sympatric endemic diseases in Venezuela. Parasite 8 :355–357.
Zijlstra E, Nur Y, Desjeux P, Khalil E, El-Hassan A, Groen J, 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Med Int Health 6 :108–113.
Jelinek T, Eichenlaub S, Losher T, 1999. Sensitivity and specificity of a rapid immunochromatographic test for diagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis 18 :669–670.
Dietze R, Barros GB, Teixeira L, Harris J, Michelson K, Falqueto A, Corey R, 1997. Effect of eliminating seropositive canines on the transmission of visceral leishmaniasis in Brazil. Clin Infect Dis 25 :1240–1242.
Pintado V, Lopez-Velez R, 2001. HIV-associated visceral leishmaniasis. Clin Microbiol Infect 7 :291–300.
Houghton R, Petrescu M, Benson D, Skeiky Y, Scalone A, Badaro R, Reed S, Gradoni L, 1998. A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type I patients and a prognostic indicator for monitoring patients undergoing drug therapy. J Infect Dis 177 :1339–1344.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 257 | 186 | 10 |
Full Text Views | 483 | 2 | 0 |
PDF Downloads | 104 | 2 | 0 |