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Genetic diversity of Plasmodium falciparum field samples from an isolated Colombian village.
Diana Gómez
Diana Gómez
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
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Jacqueline Chaparro
Jacqueline Chaparro
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
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Claudia Rubiano
Claudia Rubiano
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
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MarÃa Orfa Rojas
MarÃa Orfa Rojas
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
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Moises Wasserman
Moises Wasserman
Laboratory of Biochemistry, Instituto Nacional de Salud, Bogota, Colombia.
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Colombian field isolates of Plasmodium falciparum were analyzed for genetic diversity. Fifty-three samples were collected as thick smears from patients living in PanguÃ, an isolated area with low migration. While the samples were being collected, Panguà was experiencing an epidemic outbreak of malaria. The samples were typified using nested polymerase chain reaction (PCR) amplification of block 2 of the merozoite surface protein 1 (MSP1) gene and nested PCR with mutation-specific primers for position 108 of the dihydrofolate reductase enzyme gene. The results for the circulating population of parasites in Panguà show low diversity--four allelic forms--using MSP1 as a marker, a fact that contrasts with data reported for certain Asian and African zones. A high percentage of mixed infections was observed, as was high complexity of the infection. No differential distributions were found for any allelic type.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 52 | 52 | 15 |
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